Application of Saliva as Diagnostic Bio-Fluid- An Overview

Rathee, Manu; Bhoria, Mohaneesh

doi:10.18488/journal.98/2014.1.1/98.1.13.17

Cited by 1 publication

(1 citation statement)

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“…Salivary fluid component comprised of gingival crevicular fluid also contains serum exudates. This fluid was further mixed with other fluids from salivary glands and bronchial and nasal secretions (37) which could result in analyte dilution. Therefore, it is possible that the concentration or activity of the salivary oxidative indices and cytokine were lower compared with findings reported with plasma levels (38).…”

Section: Discussionmentioning

confidence: 99%

Non-invasive Sampling for Assessment of Oxidative Stress and Pro-inflammatory Cytokine Levels in Beta-Thalassaemia Major Patients

Rahim

Tan

Mani

et al. 2016

Revista Romana De Medicina De Laborator

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Background: Beta-thalassaemia (β-thalassaemia) major patients are severely anaemic and require life-long blood transfusions for survival. These patients require iron-chelation therapy as a result of iron overload due to the monthly blood transfusions. The iron over load can cause oxidative damage and pro-inflammation and therefore, hasten mortality. Thus, regular monitoring of the oxidative stress and pro-inflammation status may be useful in these patients. Methods: Measurement of biomarkers is usually performed on serum samples but the evaluation in non-invasive samples such as saliva would be more favourable in paediatric cases. In this study, the levels of pro-inflammatory cytokines such as tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as well as oxidative indices such as lipid hydro peroxide, advanced oxidation protein products (AOPP), ferric reducing antioxidant power (FRAP), uric acid (UA) and glutathione peroxidase (GPx) activity in a total of 65 β-thalassaemia major patients (all on iron chelation) and 55 healthy control subjects were assessed. All the above biochemical parameters, measured using well established assay techniques, were detectable in saliva samples. Results: Non parametric analyses showed that lipid hydroperoxide (LH) and glutathione peroxidase (GPx) activities were significantly higher in β-thalassaemia major patients. All other parameters were not significantly different between patient and control groups implying that iron chelation therapy was successful in attenuating oxidative stress. Strong positive correlation was observed between FRAP and UA levels. There was also a notable difference in tumour necrosis factor-α (TNF-α) between the patients and healthy controls when analysed according to ethnicity and age. AOPP level in β+-thalassaemia homozygous patients were significantly higher than β+/β0-compound heterozygous and β0-thalassaemia homozygous patients. Conclusion: Saliva may serve as a reliable, non-invasive sample which can be used to assess oxidative indices and pro-inflammatory cytokines in β-thalassaemia major patients.

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Section: Discussionmentioning

confidence: 99%