Application of Robotics in Blood Banking

Friedman, Leonard I.; Severns, S. M. L.

doi:10.1007/978-1-4613-2329-7_17

Cited by 2 publications

(1 citation statement)

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“…Laboratories engaged in automation that utilize robotic workstations equipped with nondisposable tips have adopted different approaches to prevent carryover. Researchers have used an excess volume of water to flush out potential contaminants (7,19,36,48–52) or have used a diluted version of RoboScrub (TECAN product) for cleaning the tips (53). Some researchers have also incorporated a 0.5% or 1% sodium hypochlorite tip wash step at the start of each day as a preventive measure to potential carry‐over (7,36).…”

Section: Discussionmentioning

confidence: 99%

Automated Processing of Forensic Casework Samples Using Robotic Workstations Equipped with Nondisposable Tips: Contamination Prevention

Frégeau

Lett

Elliott

et al. 2008

Journal of Forensic Sciences

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An automated process has been developed for the analysis of forensic casework samples using TECAN Genesis RSP 150/8 or Freedom EVO liquid handling workstations equipped exclusively with nondisposable tips. Robot tip cleaning routines have been incorporated strategically within the DNA extraction process as well as at the end of each session. Alternative options were examined for cleaning the tips and different strategies were employed to verify cross-contamination. A 2% sodium hypochlorite wash (1/5th dilution of the 10.8% commercial bleach stock) proved to be the best overall approach for preventing cross-contamination of samples processed using our automated protocol. The bleach wash steps do not adversely impact the short tandem repeat (STR) profiles developed from DNA extracted robotically and allow for major cost savings through the implementation of fixed tips. We have demonstrated that robotic workstations equipped with fixed pipette tips can be used with confidence with properly designed tip washing routines to process casework samples using an adapted magnetic bead extraction protocol.

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Section: Discussionmentioning

confidence: 99%