Application of RNA Interference to Root-Knot Nematode Genes Encoding Esophageal Gland Proteins

Rosso, Marie‐Noëlle; Dubrana, M. P.; Cimbolini, Nicolas; Jaubert, Stéphanie; Abad, Pierre

doi:10.1094/mpmi-18-0615

Cited by 146 publications

(117 citation statements)

References 20 publications

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“…Park et al (2008) compared three methods of transferring dsRNA into B. xylophilus and found that microinjection was the most efficient, compared to soaking and electroporation. Octopamine, resorcinol and serotonin were used to induce ingestion of dsRNA from the soaking buffer by plant parasitic root-knot nematodes (Urwin et al, 2002;Rosso et al, 2005;Bakhetia et al, 2007;Shingles et al, 2007). The addition of spermidine to the soaking buffer and an extended incubation time were reported to increase the efficiency of RNAi for Globodera rostochiensis and B. xylophilus (Chen et al, 2005;Park et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Nematodes of various developmental stages were separated from the B. cinerea hyphae in a Baermann funnel for 6 h at 25°C, placed into 10-mL tubes, and centrifuged at 2000 rpm for 5 min. The nematodes were washed five to six times with M9 buffer (Rosso et al, 2005) to remove any trace of B. cinerea mycelium, and then incubated at 20°C at 175 rpm for 24 h; the nematodes were then maintained at -70°C until use.…”

Section: Biological Materialsmentioning

confidence: 99%

See 1 more Smart Citation

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Lu²,

Jin

et al. 2011

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Section: Biological Materialsmentioning

confidence: 99%

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Lu²,

Jin

et al. 2011

Genet. Mol. Res.

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“…RNAi can be induced in M. incognita by feeding, with variable silencing efficiencies depending on the gene target 35,36 . M. incognita has many genes of the C. elegans RNAi pathway, including components of the amplification complex (ego-1, rrf-1, rrf-2 and rrf-3).…”

Section: Protection Against Environmental Stressesmentioning

confidence: 99%

Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita

et al. 2008

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Plant-parasitic nematodes are major agricultural pests worldwide and novel approaches to control them are sorely needed. We report the draft genome sequence of the root-knot nematode Meloidogyne incognita, a biotrophic parasite of many crops, including tomato, cotton and coffee. Most of the assembled sequence of this asexually reproducing nematode, totaling 86 Mb, exists in pairs of homologous but divergent segments. This suggests that ancient allelic regions in M. incognita are evolving toward effective haploidy, permitting new mechanisms of adaptation. The number and diversity of plant cell wall-degrading enzymes in M. incognita is unprecedented in any animal for which a genome sequence is available, and may derive from multiple horizontal gene transfers from bacterial sources. Our results provide insights into the adaptations required by metazoans to successfully parasitize immunocompetent plants, and open the way for discovering new antiparasitic strategies.Plant-parasitic nematodes are responsible for global agricultural losses amounting to an estimated $157 billion annually. Although chemical nematicides are the most reliable means of controlling root-knot nematodes, they are increasingly being withdrawn owing to their toxicity to humans and the environment. Novel and specific targets are thus needed to develop new strategies against these pests.The Southern root-knot nematode Meloidogyne incognita is able to infect the roots of almost all cultivated plants, making it perhaps the

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“…Resorcinol has also recently been used to stimulate uptake of solution by J2 RKNs (Huang et al, 2006;Rosso et al, 2005), and thus freshly hatched M. hapla J2 were first treated with a solution of 0.01% FDA and 1% resorcinol. Similar to that described by Bird (1979), discrete fluorescent particles were observed within M. hapla J2 nematodes, however, fluorescent precipitates also appeared in the solution during treatment and the treated nematodes appeared to lose active movement.…”

Section: Low Concentrations Of Fda Marked Live Rknsmentioning

confidence: 99%

“…Live cyst nematodes have recently been marked fluorescently using fluorescein isothiocyanate (FITC) (Schroeder and MacGuidwin, 2007). FITC is a fluorescent fluorescein conjugate that has also been used to show uptake from solution by J2 RKNs (Rosso et al, 2005). An earlier study made use of a different fluorescein conjugate, fluorescein diacetate (FDA), to label RKN juveniles and to distinguish between living and dead nematodes (Bird, 1979).…”

mentioning

confidence: 99%

In planta observation of live fluorescent plant endoparasitic nematodes during early stages of infection

Goto¹,

Fosu-Nyarko²,

Sakuma³

et al. 2010

Japanese Journal of Nematology

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Plant endoparasitic nematodes spend most of their life cycle hidden within plant roots and worldwide cause major losses to agriculture every year. Root-knot nematodes ('RKNs', Meloidogyne spp.) are sedentary endoparasitic nematodes that establish permanent feeding sites within plant roots as 2nd-stage juveniles (J2), and then spend the rest of their life-cycle at this site. In contrast, root-lesion nematodes (Pratylenchus spp.) are migratory endoparasites that move in roots and feed on different root cells, causing typical symptoms of brown lesions.There is great interest in studying the initial infection stages of both sedentary and migratory endoparasitic nematodes. In the case of RKNs, it is known that host cells undergo key initial changes of cell-cycle activation, DNA synthesis and nuclear division during the first 48 hr of infection and host cell selection (de Almeida Engler et al., 1999;Jones, 1981;Jones and Payne, 1978;Niebel et al., 1996). In the case of root-lesion nematodes, tracking invasive movement and behavior in root tissues also has potential to reveal the nature of resistant responses limiting the completion of the life-cycle. A major challenge is that current methods to identify nematodes inside plant roots during early stages of infection are either destructive or do not provide sufficient temporal or spatial resolution.Fluorescent compounds have been used to track solute movement in host plant tissues during parasitic nematode infection (Böckenhoff et al., 1996;Hofmann et al., 2007;Hutangura, 1999). Expression of fluorescent proteins has also been used to study feeding cell biology (Hofmann and Grundler, 2006;Hoth et al., 2005;Hoth et al., 2008) and is a common approach to identify specific cell types in plant roots (Birnbaum et al., 2003;Brady et al., 2007;Lee et al., 2006). Research on initial stages of nematode infection would thus greatly benefit from a fluorescence-based approach that would also enable invading nematodes to be identified in plant roots with high spatial resolution. Live cyst nematodes have recently been marked fluorescently using fluorescein isothiocyanate (FITC) (Schroeder and MacGuidwin, 2007). FITC is a fluorescent fluorescein conjugate that has also been used to show uptake from solution by J2 RKNs (Rosso et al., 2005). An earlier study made use of a different fluorescein conjugate, fluorescein diacetate (FDA), to label RKN juveniles and to distinguish between living and dead nematodes (Bird, 1979). FDA is a non-fluorescent conjugate that becomes fluorescent after entering a cell and being hydrolyzed by endogenous esterases. In addition to facilitating identification of live nematodes, background fluorescent signals and requirement for extensive washing are kept to a minimum because FDA does not fluoresce, and free fluorescein remains in live cells. In this study, we tested whether FDA can be applied to label individual nematodes fluorescently for direct non-destructive observation of their movement inside plant roots. MATERIALS AND METHODS Nematode stocks and FDA t...

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Application of RNA Interference to Root-Knot Nematode Genes Encoding Esophageal Gland Proteins

Cited by 146 publications

References 20 publications

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Functional analysis of the cellulose gene of the pine wood nematode, Bursaphelenchus xylophilus, using RNA interference

Genome sequence of the metazoan plant-parasitic nematode Meloidogyne incognita

In planta observation of live fluorescent plant endoparasitic nematodes during early stages of infection

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