Application of Rice Root Endophytic Bacteria in Ciherang Variety Rice (Oryza sativa) Seeds

Abstract

Endophytic bacteria are microorganisms that thrive in plant tissue without causing any negative effects to the host plants. The purpose of this study was to apply endophytic bacteria that have the potential to produce IAA hormones as biological agents in rice seeds. This research was conducted by testing IAA hormone using a spectrophotometer, testing HCN production using filter paper, biochemically identification of the isolate, and applicating endophytic bacteria into rice seeds. Endophytic bacteria have different abilities in producing IAA hormone. Based on the results of the study, the highest IAA concentration (57 ppm) was obtained from KSB1 (Potassium Solubilizing Bacteria) isolates and followed by KSB3 isolate with 56 ppm. All the endophytic bacteria isolates were unable to produce HCN. Results of the study showed that KSB1 and KSB3 isolate were able to influence the growth of root length by 5.62 cm and 6.70 respectively as well as the canopy height by 8.56 cm and 8.30 cm respectively. Based on observations of colony morphology, cell morphology and biochemical tests, KSB1 was presumably belong to the genus Staphylococcus. In this study, new information about endophytic bacteria that can increase the growth of Ciherang variety rice can be obtained. This study provides information that the application of environmentally friendly bacteria is important to preserve the environment and agriculture sustainability in Indonesia without reducing the productivity of rice.

In vitro screening of siderophore-producing rice root endophytic bacteria from up-land paddies in north-western Vietnam for plant growth-promoting activities

“…Applying agrochemicals in paddies is considered an easy solution to control diseases and maintain high yield; however, the overuse of these chemicals might cause tremendous impact on environment, public health and non-target organisms due to the high levels of toxic residues (Ali et al, 2017). At the same time, prolonged agrochemical exposure of paddies also reduces their effectiveness and might increase the number of insecticide-resistant strains (Fitri et al, 2020). In Vietnam, during the period of 1995-2018, the positive correlations between the amount of rice production and the use of pesticides indicated the intractable routines of farmers in using of pesticides for rice production.…”

“…Thus, alternative solutions are being developed to alleviate the dependence of agrochemicals. Beneficial endophytic bacteria (EB) have been studied as an alternative measure to improve crop yield, grain quality and to prevent pathogens; at the same time, the use of endophytes does not cause serious damage to the crops and ecosystem (Moronta-Barrios et al, 2018;Fitri et al, 2020).…”