Application of rice endophytic <i>Bradyrhizobium</i> strain SUTN9-2 containing modified ACC deaminase to rice cultivation under water deficit conditions

Sarapat, Sukanlaya; Longtonglang, Aphakorn; Umnajkitikorn, Kamolchanok; Girdthai, Teerayoot; Boonkerd, Nantakorn; Tittabutr, Panlada; Teaumroong, Neung

doi:10.1080/17429145.2020.1824028

Cited by 10 publications

(5 citation statements)

References 56 publications

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“…The potential ACCD positive endophytes reported in rice so far include Methylobacterium oryzae (Chinnadurai et al,2009), Bacillus altitudinis (Kumar et al,2017), Pseudomonas fluorescens (Etesami et al,2014); Bradyrhizobium sp. (Sarapat et al,2020), Brevibacterium linens (Choi et al,2022), Bacillus amyloliquefaciens (Liu et al,2022). In the present work, we reported Bacillus cereus (NE07) and Priestia endophytica (NE14) as potential ACC deaminase-producing endophytes of Norungan.…”

Section: Drought-mitigationsupporting

confidence: 59%

Drought-Tolerant Landrace Rice Norungan Harbors Novel Putative Competitive Endophytes

Nunna¹,

Balachandar²

2022

MAJ

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The landraces and wild species wisely choose their microbiome and explored them as a second genome for nutrient acquisition from the limited resources of the soil ecosystem and can withstand drought and diseases. Hence, the present study aimed to explore the drought-tolerant rice landrace of Tamil Nadu for the putative competitive endophytes to develop a plant-growth-promoting endophytic bacterial inoculant. Twenty two putative competitive endophytes were authenticated from plant tissues and seeds of Norungan. The 16S rRNA gene sequencing revealed that Norungan rice was colonized predominantly by Firmicutes, including six species of Bacillus, four species of Priestia, and one species each of Micrococcus, Geobacillus, and Lysinibacillus, along with two Proteobacteria, Enterobacter cloacae and Kosakonia oryzae. These endophytes were screened for nutrient transformation, growth hormone production, drought mitigation, and antagonism against plant pathogens. Bacillus cereus NE07, B. paralicheniformis NE18, B. subtilis NE20, and Priestia flexa NE09 showed potential nutrient-transforming capability, while Bacillus cereus NE07 and Priestia flexa NE09 showed high plant growth hormones (indole-3-acetic acid and gibberellic acid production). Similarly, Bacillus cereus NE07 and Priestia endophytica (NE14) showed high ACC deaminase, proline accumulation, biofilm production, and potential candidates for drought mitigation. Bacillus hayneshii NE04 and Priestia aryabhattai NE10, and Bacillus cereus NE07 showed antagonism against fungal pathogen Rhizoctonia solani and bacterial leaf blight causing Xanthomonas oryzae. The results confirmed that no single strain with all plant-growth-promoting and stress-mitigating traits found in Norungan, and these strains were performed division of labor basis. Hence, developing a putative competitive endophytic bacterial consortium with compatible strains representing cumulative beneficial traits as inoculants would be a novel approach for improving rice productivity

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Section: Drought-mitigationsupporting

confidence: 59%

Drought-Tolerant Landrace Rice Norungan Harbors Novel Putative Competitive Endophytes

Nunna¹,

Balachandar²

2022

MAJ

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“…Pongdet et al also demonstrated that rice cultivars can regulate their association with endophytic bradyrhizobia [ 46 ]. Endophytic Bradyrhizobium in cultivated rice may assist plants in absorbing or utilizing nitrogen that can be used for plant growth-related processes, such as in photosynthesis in leaves [ 53 , 55 ]. This study showed that cultivated rice accessions had higher relative abundance of Bradyrhizobium than wild rice accessions did (Fig.…”

Section: Discussionmentioning

confidence: 99%

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

et al. 2021

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Background Rice, which serves as a staple food for more than half of the world’s population, is grown worldwide. The hybridization of wild and cultivated rice has enabled the incorporation of resistance to varying environmental conditions. Endophytic microbiota are known to be transferred with their host plants. Although some studies have reported on the endophytic microbiota of wild and cultivated rice, the inheritance from wild and cultivated rice accessions in next generations, in terms of endophytic microbiota, has not been examined. Results In the present study, the endophytic microbial community structures of Asian and African wild and cultivated rice species were compared with those of their F1 offspring. High-throughput sequencing data of bacterial 16S rDNA and fungal internal transcribed spacer regions were used to classify the endophytic microbiota of collected samples of rice. Results indicated that when either African or Asian wild rice species were crossed with cultivated rice accessions, the first generation harbored a greater number of root endophytic fungi than the cultivated parent used to make the crosses. Network analysis of the bacterial and fungal operational taxonomic units revealed that Asian and African wild rice species clustered together and exhibited a greater number of significant correlations between fungal taxa than cultivated rice. The core bacterial genus Acidovorax and the core fungal order Pleosporales, and genera Myrothecium and Bullera connected African and Asian wild rice accessions together, and both the wild rice accessions with their F1 offspring. On the other hand, the core bacterial genus Bradyrhizobium and the core fungal genera Dendroclathra linked the African and Asian cultivated rice accessions together. Conclusions This study has theoretical significance for understanding the effect of breeding on the inheritance of endophytic microbiota of rice and identifying beneficial endophytic bacteria and fungi among wild and cultivated rice species, and their F1 offspring.

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“…( Ji et al, 2020 ) and Burkholderia sp. ( Sarkar et al, 2018 ) inoculation has been reported to induce salt stress tolerance, while Bradyrhizobium SUTN9-2 ( Sarapat et al, 2020 ) enhanced rice growth under drought stress condition in rice. Submergence tolerance also have been induced in rice treated with ACC deaminase producing Microbacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Silicate solubilizing and plant growth promoting bacteria interact with biogenic silica to impart heat stress tolerance in rice by modulating physiology and gene expression

Chaganti,

Phule,

Chandran

et al. 2023

Front. Microbiol.

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Heat stress caused due to increasing warming climate has become a severe threat to global food production including rice. Silicon plays a major role in improving growth and productivity of rice by aiding in alleviating heat stress in rice. Soil silicon is only sparingly available to the crops can be made available by silicate solubilizing and plant-growth-promoting bacteria that possess the capacity to solubilize insoluble silicates can increase the availability of soluble silicates in the soil. In addition, plant growth promoting bacteria are known to enhance the tolerance to abiotic stresses of plants, by affecting the biochemical and physiological characteristics of plants. The present study is intended to understand the role of beneficial bacteria viz. Rhizobium sp. IIRR N1 a silicate solublizer and Gluconacetobacter diazotrophicus, a plant growth promoting bacteria and their interaction with insoluble silicate sources on morpho-physiological and molecular attributes of rice (Oryza sativa L.) seedlings after exposure to heat stress in a controlled hydroponic system. Joint inoculation of silicates and both the bacteria increased silicon content in rice tissue, root and shoot biomass, significantly increased the antioxidant enzyme activities (viz. superoxidase dismutase, catalase and ascorbate peroxidase) compared to other treatments with sole application of either silicon or bacteria. The physiological traits (viz. chlorophyll content, relative water content) were also found to be significantly enhanced in presence of silicates and both the bacteria after exposure to heat stress conditions. Expression profiling of shoot and root tissues of rice seedlings revealed that seedlings grown in the presence of silicates and both the bacteria exhibited higher expression of heat shock proteins (HSPs viz., OsHsp90, OsHsp100 and 60 kDa chaperonin), hormone-related genes (OsIAA6) and silicon transporters (OsLsi1 and OsLsi2) as compared to seedlings treated with either silicates or with the bacteria alone. The results thus reveal the interactive effect of combined application of silicates along with bacteria Rhizobium sp. IIRR N1, G. diazotrophicus inoculation not only led to augmented silicon uptake by rice seedlings but also influenced the plant biomass and elicited higher expression of HSPs, hormone-related and silicon transporter genes leading to improved tolerance of seedling to heat stress.

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Application of rice endophytic Bradyrhizobium strain SUTN9-2 containing modified ACC deaminase to rice cultivation under water deficit conditions

Cited by 10 publications

References 56 publications

Drought-Tolerant Landrace Rice Norungan Harbors Novel Putative Competitive Endophytes

Drought-Tolerant Landrace Rice Norungan Harbors Novel Putative Competitive Endophytes

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

Silicate solubilizing and plant growth promoting bacteria interact with biogenic silica to impart heat stress tolerance in rice by modulating physiology and gene expression

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