Application of ribotyping for differentiating Vibrio cholerae non-O1 isolated from shrimp farms in Thailand

Dalsgaard, Anders; Echeverria, Peter; Larsen, J. L.; Siebeling, R. J.; Serichantalergs, Oralak; Huss, Hans Henrik

doi:10.1128/aem.61.1.245-251.1995

Cited by 43 publications

(36 citation statements)

References 44 publications

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“…Repeated studies of all strains showed no variation in ribotype patterns, although differences in band intensity and the degree of background were observed for a few strains. A high degree of reproducibility of the BglI ribotypes of V. cholerae has been reported previously (6,22). The ribotype patterns produced with the restriction enzyme HindIII were identical for the 19 strains studied (data not shown).…”

Section: Resultssupporting

confidence: 60%

“…The ribotype patterns produced with the restriction enzyme HindIII were identical for the 19 strains studied (data not shown). Thus, our study provides further evidence that BglI is suitable for use in ribotyping V. cholerae strains (5)(6)(7)22).…”

Section: Resultssupporting

confidence: 56%

“…Total bacterial DNA was extracted by the method of Murray and Thompson (19). On the basis of previous studies (6,22), the restriction enzyme BglI was used to digest chromosomal DNA. In addition, the enzyme HindIII was used to digest the DNA to be used for ribotyping.…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…An alkali blotting procedure (0.4 M NaOH) and a vacuum blotter (LKB, Bromma, Sweden) were used. The membranes were air dried prior to hybridization (6).…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

et al. 1996

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In the present study, 19 strains of Vibrio cholerae O1 biotype El Tor isolated during outbreaks of cholera in Guinea-Bissau in 1987, 1994, and 1995 were characterized to investigate a possible epidemiological relationship among the isolates. On the basis of ribotyping with the restriction enzyme BglI, 5 strains isolated in 1987 showed two closely related ribotypes, while 14 strains isolated in 1994 and 1995 showed the same ribotype that was distinct from the ribotypes of strains isolated in 1987. Southern blot hybridization of BglI-digested genomic DNA with a cholera toxin probe demonstrated that the strains isolated in 1987 showed an identical cholera toxin genotype, whereas O1 strains isolated in 1994 and 1995 showed the same genotype that was distinct from the genotype of strains isolated in 1987. These results were supported by the results of antibiotic susceptibility testing, in which strains isolated in 1987 showed resistance to polymyxin B only, while each of the strains from 1994 and 1995 showed resistance to polymyxin B, trimethoprim-sulfamethoxazole, and the vibriostatic agent O/129. Although our results are based on a limited number of V. cholerae O1 strains, they suggest that the epidemic in Guinea-Bissau in 1994 and 1995 was due to the introduction of a new strain to the country.

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Section: Resultssupporting

confidence: 60%

Section: Resultssupporting

confidence: 56%

Section: Bacterial Strainsmentioning

confidence: 99%

“…An alkali blotting procedure (0.4 M NaOH) and a vacuum blotter (LKB, Bromma, Sweden) were used. The membranes were air dried prior to hybridization (6).…”

Section: Bacterial Strainsmentioning

confidence: 99%

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Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

et al. 1996

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“…Vibrio alginolyticus (George et al 2005); Vibrio parahaemolyticus and V. alginolyticus (Sudheesh et al 2002); V. parahaemolyticus (Maluping et al 2005). Dalsgaard et al (1995) applied ribotyping to differentiate V. cholerae non-O1 isolated from shrimp farms in Thailand. The genetic diversity of V. cholerae was determined by multilocus enzyme electrophoresis (Desmarchelier et al 1988;Chen et al 1991), ribotyping (Koblavi et al 1990;Karaolis et al 1994), pulsed-field gel electrophoresis (Cameron et al 1994) and amplified fragment length polymorphism fingerprinting (Jiang et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches

Rao

Surendran

2010

Letters in Applied Microbiology

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Aims: The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequences. Methods and Results: Non‐O1 and non‐O139 V. cholerae isolates were obtained from shrimp pond water, pond sediment, shrimp head and shrimp muscle. RS‐PCR yielded fewer bands than REP‐PCR and ERIC‐PCR. Higher similarity was observed in RS‐PCR (75–100%) than in REP‐PCR (60–95%) and ERIC‐PCR (40–95%). Conclusions: A 100% similarity between V. cholerae isolates was only noticed in RS‐PCR. The choleratoxigenic V. cholerae (non‐O1 and non‐O139) showed greater genetic similarity with ctx‐negative V. cholerae than among ctx‐positive V. cholerae. Significance and Impact of the Study: The greater similarity of ctx‐positive V. cholerae with ctx‐negative V. cholerae isolates indicates that the ctx‐positive strains (non‐O1 and non‐O139) might have originated from autochthonous V. cholerae in the aquatic niche.

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Differentiation of Rhodococcus species by ribotyping

Jorks

1996

J. Basic Microbiol.

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The ribosomal RNA gene restriction patterns (ribotyping) was investigated with respect to the characterization of Rhodococcus species and R. rhodochrous strains. Chromosomal DNA was prepared, digested with BamH I, blotted, and hybridized with acetylaminofluorene-labelled 16 + 23S rRNA from E. coli. The type strains of seven Rhodococcus species studied gave different hybridization patterns in each case. All strains tested were clearly distinguishable by ribotyping. Patterns contained two to eight bands between 1.4 kb and 11.8 kb and demonstrated the high genetic divergence of genus Rhodococcus. Investigation of nine R. rhodochrous strains resulted in patterns with seven or eight bands. One fragment, 4.4 kb in size, was common to all R. rhodochrous strains and appears to be characteristic of R. rhodochrous. Ribotyping was evaluated as a tool for distinguishing between Rhodococcus species.

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Application of ribotyping for differentiating Vibrio cholerae non-O1 isolated from shrimp farms in Thailand

Cited by 43 publications

References 44 publications

Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

Molecular characterization of Vibrio cholerae O1 strains isolated during cholera outbreaks in Guinea-Bissau

Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches

Differentiation of Rhodococcus species by ribotyping

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