Application of reverse micelle extraction process for amylase recovery using response surface methodology

Bera, Manab Bandhu; Panesar, Parmjit S.; Panesar, Reeba; Singh, Balbir Singh Mahinder

doi:10.1007/s00449-007-0172-6

Cited by 17 publications

(8 citation statements)

References 13 publications

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“…This decrease in positive charge in turn reduces electrostatic interaction between the negatively charged surfactant head group and target protein. Similar observation of decrease in extraction of target protein as the pH reaches to its isoelectric point was reported for papain,27 amylase,26 and nattokinase 28…”

Section: Resultssupporting

confidence: 81%

“…The decrease in activity recovery beyond pH 6.0 is due to decrease in electrostatic interaction and in charge density on lactoperoxidase as pH reaches to its isoelectric point. Bera et al26 reported that as the pH of the protein approaches its isoelectric point the positive charge density of the protein decreases. This decrease in positive charge in turn reduces electrostatic interaction between the negatively charged surfactant head group and target protein.…”

Section: Resultsmentioning

confidence: 99%

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Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction

Nandini

Rastogi

2010

Biotechnology Progress

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The extraction of lactoperoxidase (EC 1.11.1.7) from whey was studied using single step reverse micelles-assisted extraction and compared with reverse micellar extraction. The reverse micelles-assisted extraction resulted in extraction of contaminating proteins and recovery of lactoperoxidase in the aqueous phase leading to its purification. Reverse micellar extraction at the optimized condition after forward and backward steps resulted in activity recovery of lactoperoxidase and purification factor of the order of 86.60% and 3.25-fold, respectively. Whereas reverse micelles-assisted extraction resulted in higher activity recovery of lactoperoxidase (127.35%) and purification factor (3.39-fold). The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles also evidenced that higher purification was obtained in reverse micelles-assisted extraction as compared of reverse micellar extracted lactoperoxidase.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction

Nandini

Rastogi

2010

Biotechnology Progress

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“…A second-order polynomial equation model was used to fit the Y and PF data with interaction terms as given below: Y i = A 0 + A i Σx i + A ij Σx i x j + A ii Σx i 2 (i≠j), where Y i is the predicted response value; A 0 , A i , A ij and A ii are the regression coefficients of the model for the intercept, linear, cross-product and quadratic terms, respectively; and x i is the variable studied [ 47 , 48 ].…”

Section: Methodsmentioning

confidence: 99%

Separation and Enrichment of Lectin from Zihua Snap-Bean (Phaseolus vulgaris) Seeds by PEG 600–Ammonium Sulfate Aqueous Two-Phase System

et al. 2017

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A fast and efficient method based on a polyethylene glycol (PEG) 600/(NH4)2SO4 aqueous two-phase system for extracting lectin from Zihua snap-bean (Phaseolus vulgaris) seeds was established. According to a Box–Behnken design (BBD), involving four factors at three levels each subjected to analysis of variance (ANOVA) and response surface analysis, the protein recovery and the purification factor of lectin in the top phase were used as the response values of the variance analysis to acquire the multivariate quadratic regression model. SDS–PAGE electrophoresis and the hemagglutination test were used to detect the distribution of lectin in the aqueous two-phase system (ATPS). The obtained data indicated that lectin was preferentially partitioned into the PEG-rich phase, and the ATPS, composed of 15% (NH4)2SO4 (w/w), 18% PEG 600 (w/w), 0.4 g/5 g NaCl and 1 mL crude extract, showed good selectivity for lectin when the pH value was 7.5. Under the optimal conditions, most of the lectin was assigned to the top phase in the ATPS, and the hemagglutination activity of the purified lectin in the top phase was 3.08 times that of the crude extract. Consequently, the PEG 600/(NH4)2SO4 aqueous two-phase system was an effective method for separating and enriching lectin directly from the crude extract of Zihua snap-bean seeds.

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“…1) for the effects of the interaction variables on diosgenin yield were generated to determine the interactions among these factors and their optimum concentration values. In the plots, the responses were studied taking two variables at a time while keeping the other one at the '0' level [16][17][18][19][20][21][22][23]. Figure 1b showed the interaction of peptone and Tween 80.…”

Section: Optimization Of Medium Compositionmentioning

confidence: 99%

“…On the other hand, response surface methodology (RSM) could be used for such purposes. Hitherto, RSM has been widely used in the optimization of microbial transformation process by building models, designing experiments, evaluating the effects of variables and optimizing conditions [16][17][18][19][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Process optimization for the production of diosgenin with Trichoderma reesei

Zhu

Huang

2009

Bioprocess Biosyst Eng

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Based on the response surface methodology, an effective microbial system for diosgenin production from enzymatic pretreated Dioscorea zingiberensis tubers with Trichoderma reesei was studied. The fermentation medium was optimized with central composite design (3(5)) depended on Plackett-Burmann design which identified significant impacts of peptone, K(2)HPO(4) and Tween 80 on diosgenin yield. The effects of different fermentation conditions on diosgenin production were also studied. Four parameters, i.e. incubation period, temperature, initial pH and substrate concentration were optimized using 4(5) central composite design. The highest diosgenin yield of 90.57% was achieved with 2.67% (w/v) of peptone, 0.29% (w/v) of K(2)HPO(4), 0.73% (w/v) of Tween 80 and 9.77% (w/v) of substrate, under the condition of pH 5.8, temperature 30 degrees C. The idealized incubation time was 6.5 days. After optimization, the product yield increased by 33.70% as compared to 67.74 +/- 1.54% of diosgenin yield in not optimized condition. Scale-up fermentation was carried out in a 5.0 l bioreactor, maximum diosgenin yield of 90.17 +/- 3.12% was obtained at an aeration of 0.80 vvm and an agitation rate of 300 rpm. The proposed microbial system is clean and effective for diosgenin production and thus more environmentally acceptable than the traditional acid hydrolysis.

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Application of reverse micelle extraction process for amylase recovery using response surface methodology

Cited by 17 publications

References 13 publications

Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction

Single step purification of lactoperoxidase from whey involving reverse micelles‐assisted extraction and its comparison with reverse micellar extraction

Separation and Enrichment of Lectin from Zihua Snap-Bean (Phaseolus vulgaris) Seeds by PEG 600–Ammonium Sulfate Aqueous Two-Phase System

Process optimization for the production of diosgenin with Trichoderma reesei

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