Application of Restriction Fragment Length Polymorphism to Maize Breeding

Murray, Michael G.; Chyi, Y.-S.; Cramer, Jane Harris; Demars, Sandra; Kirschman, Jane; Ma, Yue; Pitas, Jan W.; Romero‐Severson, Jeanne; Shoemaker, Jennifer; West, David; Zaitlin, Dave

doi:10.1007/978-1-4615-3304-7_26

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…The presence of duplicated genes in maize has been observed for loci controlling morphological traits (26), isozyme loci (27), and RFLP loci (28,29). The mechanism by which these duplications originated is not known, but mapping experiments with RFLP loci have indicated that they are usually duplicated on separate chromosomes and that large segments of different chromosomes sometimes contain these duplicated loci in the same linear order.…”

Section: Discussionmentioning

confidence: 99%

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Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes.

Hulbert

Richter

Axtell

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

265

119

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Cloned DNA fragments from 14 characterized maize genes and 91 random fragments used for genetic mapping in maize were tested for their ability to hybridize and detect restriction fragment length polymorphisms in sorghum and other related crop species. Most DNA fragments tested hybridized strongly to DNA from sorghum, foxtail millet, Johnsongrass, and sugarcane. Hybridization to pearl millet DNA was generally weaker, and only a few probes hybridized to barley DNA under the conditions used. Patterns of hybridization of low-copy sequences to maize and sorghum DNA indicated that the two genomes are very similar. Most probes detected two loci in maize; these usually detected two loci in sorghum. Probes that detected one locus in maize generally detected a single locus in sorghum. However, maize repetitive DNA sequences present on some of the genomic clones did not hybridize to sorghum DNA. Most of the probes tested detected polymorphisms among a group of seven diverse sorghum lines tested; over one-third of the probes detected polymorphism in a single F2 population from two of these lines. Cosegregation analysis of 55 F2 individuals enabled several linkage groups to be constructed and compared with the linkage relationships of the same loci in maize. The linkage relationships of the polymorphic loci in the two species were usually conserved, but several rearrangements were detected.The tribe Andropogonae of the Gramineae contains several important crop species of which maize (Zea mays) is the best characterized genetically. Cloned maize DNA fragments that detect polymorphism and have been genetically mapped are available to the public (1-3). These probes have been used to generate a genetic map (1), to estimate evolutionary relationships between related species (4) If most maize restriction fragment length polymorphism (RFLP) probes hybridize sufficiently well to sorghum DNA, then it is possible to construct a genetic map of sorghum that can be directly compared to that of maize. Cloned DNA fragments that hybridize to single sites in the genomes ofboth species can be assumed to have arisen from a single sequence in a common ancestor. The genomic position of such orthologous (6) loci in each of the two species can be compared to outline the chromosomal rearrangements that have occurred during species divergence. Sorghum and maize have the same chromosome number (n = 10), but the nuclear DNA content of maize is over 3 times that of sorghum (7).The utility of genetic mapping in related species with common RFLP probes has been demonstrated in some of the solanaceous crops (6, 8). Tomato (Lycopersicon esculentum), garden pepper (Capsicum annuum), and potato (Solanum tuberosum) all have the same basic chromosome number (n = 12) and the nucleotide sequences of most genes are conserved well enough to permit heterologous hybridization. While few differences were found between the tomato and potato genomes, numerous rearrangements characterized the linkage map of pepper.The present study was undertaken to determine the...

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Section: Discussionmentioning

confidence: 99%

“…Similarly, two maize chromosome VII locus probes identified a pair of linked loci in a linkage group composed of mostly maize chromosome II probes. Chromosomes III and VIII of maize commonly share duplicated loci as do chromosomes II and VII (28,29).…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes.

Hulbert

Richter

Axtell

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

265

119

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“…tomato mosaic virus resistance (YOUNG et al 1988), Bremia lactuca resistance (KESSELI et al 1991), rice blast resistance (Yu et al 1991) or powdery mildew {Erysiphe graminis) resistance (ScHULLER et al 1992). Markers tightly linked to a target gene are expected considerably to reduce the number of generations necessary to introduce a new gene via backcross breeding procedures (TANKSLEY et al 1989, MURRAY et al 1991.…”

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confidence: 99%

An Extended Linkage Map of Sugar Beet (Beta vulgaris L.) Including Nine Putative Lethal Genes and the Restorer Gene X

Pillen

Sleinrücken²,

Herrmann

et al. 1993

Plant Breeding

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An extended genetic map of sugar beet {Beta vulgaris L.) is presented encompassing 177 segregating markers (2 morphological traits, 7 isozymes, and 168 RFLP markers) on 9 linkage groups. The linkage map comprises 1057.3 cM equivalent to an average genetic spacing of 6.0 cM/marker. The length of individual linkage groups varies between 80.7 (group VIII) and 167.4 cM (group VII). The number of markers per linkage group ranges between 13 and 24. No indication of duplicate regions was found, confirming the true diploid nature of B. vulgaris. Twenty-six markers (15 %) deviated significantly (a = 0.01) from the expected segregation ratio. This distorted segregation was probably caused by Hnkage with lethal genes. Four such genes (designated Let lb. Let 5b, Let 6b, Let 8) could be located at discrete positions due to their absolute Hnkage to skewed RFLP markers. The restorer gene X has been located terminally on linkage group III, 9.6 cM distant from RFLP marker pKP1238.

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“…Examples include genes isolated by transposon tagging or by messenger selection and reverse transcription to produce a defined eDNA. In contrast, the agr probes developed and mapped by Murray et al ( 1989) are random cDNAs from tissue-specific libraries, which have been mapped with bnl and umc RFLP markers. Substantial numbers of random eDNA clones have been sequenced (Keith et al, 1993) and loci for them are being mapped (Chao et al, 1994;Personal Communication, Maize Genet.…”

Section: Genomes Of Maize and Sorghummentioning

confidence: 99%

USDA Plant Genome Research Program

Altenbach¹,

Anderson²,

Bigwood

et al. 1995

Advances in Agronomy

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USDA Plant Genome Research Program AbstractThe U.S. Congress appropriated funds in 1991 for the USDA Plant Genome Research Program, four years after its initial conception in 1987. The goal of the USDA Plant Genome Research Program is to improve plants (agronomic, horticultural, and forest tree species) by locating marker DNA or genes on chromosomes, determining gene structure, and transferring genes to improve plant performance with accompanying reduced environmental impact to meet marketplace needs and niches. The Plant Genome Research Program is one program with two parts: National Research Initiative and Plant Genome Database (PGD). The PGD is now a real and functioning information and data resource for agricultural and other plant science genome researchers, and it is in the public domain. Additional progress is given according to major plant groups. The PGD is a suite of several information products produced at the National Agricultural Library (NAL) in collaboration with the Agricultural Research Service and Forest Service species coordinators.

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Application of Restriction Fragment Length Polymorphism to Maize Breeding

Cited by 4 publications

References 18 publications

Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes.

Genetic mapping and characterization of sorghum and related crops by means of maize DNA probes.

An Extended Linkage Map of Sugar Beet (Beta vulgaris L.) Including Nine Putative Lethal Genes and the Restorer Gene X

USDA Plant Genome Research Program

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