Application of resazurin for estimating abundance of contaminant-degrading micro-organisms

Guérin,; Mondido,; McClenn,; Peasley,

doi:10.1046/j.1472-765x.2001.00916.x

Cited by 87 publications

(72 citation statements)

References 13 publications

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“…Check all connections and tubing (especially pump tubing) to detect air leaks. Oxygen intrusion can be detected by using resazurin, however this compound might interfere with the electrode-active biofilm 20 .…”

Section: Oxygen Intrusionmentioning

confidence: 99%

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Gildemyn

Luther

Andersen

et al. 2015

JoVE

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Streams such as urine and manure can contain high levels of ammonium, which could be recovered for reuse in agriculture or chemistry. The extraction of ammonium from an ammonium-rich stream is demonstrated using an electrochemical and a bioelectrochemical system. Both systems are controlled by a potentiostat to either fix the current (for the electrochemical cell) or fix the potential of the working electrode (for the bioelectrochemical cell). In the bioelectrochemical cell, electroactive bacteria catalyze the anodic reaction, whereas in the electrochemical cell the potentiostat applies a higher voltage to produce a current. The current and consequent restoration of the charge balance across the cell allow the transport of cations, such as ammonium, across a cation exchange membrane from the anolyte to the catholyte. The high pH of the catholyte leads to formation of ammonia, which can be stripped from the medium and captured in an acid solution, thus enabling the recovery of a valuable nutrient. The flux of ammonium across the membrane is characterized at different anolyte ammonium concentrations and currents for both the abiotic and biotic reactor systems. Both systems are compared based on current and removal efficiencies for ammonium, as well as the energy input required to drive ammonium transfer across the cation exchange membrane. Finally, a comparative analysis considering key aspects such as reliability, electrode cost, and rate is made.This video article and protocol provide the necessary information to conduct electrochemical and bioelectrochemical ammonia recovery experiments. The reactor setup for the two cases is explained, as well as the reactor operation. We elaborate on data analysis for both reactor types and on the advantages and disadvantages of bioelectrochemical and electrochemical systems. Video LinkThe video component of this article can be found at

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Section: Oxygen Intrusionmentioning

confidence: 99%

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Gildemyn

Luther

Andersen

et al. 2015

JoVE

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“…This blue, water-soluble, dye is reduced, irreversibly, to the pink dye resorufin by reactions associated with bacterial respiration [49]. Resorufin can itself be further reduced to the colourless hydroresorufin by the same processes.…”

Section: Experiments To Test For Magnetically Induced Convectionmentioning

confidence: 99%

Diamagnetic levitation enhances growth of liquid bacterial cultures by increasing oxygen availability

Dijkstra

Larkin

Anthony

et al. 2010

J. R. Soc. Interface.

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Diamagnetic levitation is a technique that uses a strong, spatially varying magnetic field to reproduce aspects of weightlessness, on the Earth. We used a superconducting magnet to levitate growing bacterial cultures for up to 18 h, to determine the effect of diamagnetic levitation on all phases of the bacterial growth cycle. We find that diamagnetic levitation increases the rate of population growth in a liquid culture and reduces the sedimentation rate of the cells. Further experiments and microarray gene analysis show that the increase in growth rate is owing to enhanced oxygen availability. We also demonstrate that the magnetic field that levitates the cells also induces convective stirring in the liquid. We present a simple theoretical model, showing how the paramagnetic force on dissolved oxygen can cause convection during the aerobic phases of bacterial growth. We propose that this convection enhances oxygen availability by transporting oxygen around the liquid culture. Since this process results from the strong magnetic field, it is not present in other weightless environments, e.g. in Earth orbit. Hence, these results are of significance and timely to researchers considering the use of diamagnetic levitation to explore effects of weightlessness on living organisms and on physical phenomena.

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“…Influent media in syringes was O 2 -free although not chemically reduced (i.e., media was pink (Guerin et al 2001)). However, media in columns was clear, and therefore reduced (Guerin et al 2001).…”

Section: Column Systemsmentioning

confidence: 99%

Microbial fuel cell technology for measurement of microbial respiration of lactate as an example of bioremediation amendment

et al. 2008

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Microbial fuel cell (MFC) based sensing was explored to provide for the development of an in situ bioremediation monitoring approach for substrate concentrations and microbial respiration rates. MFC systems were examined in column systems where Shewanella oneidensis MR1 used an external electron acceptor (an electrode) to metabolize lactate (a bioremediation additive) to acetate. Column systems were operated with varying influent lactate concentrations (0-41 mM) and monitored for current generation (0.01-0.39 mA). Biological current generation paralleled bulk phase lactate concentration both in the influent and in the bulk phase at the anode; current values were correlated to lactate concentration at the anode (R(2) = 0.9), The electrical signal provided real-time information for electron donor availability and biological activity. These results have practical implications for efficient and inexpensive real-time monitoring of in situ bioremediation processes where information on substrate concentrations is often difficult to obtain and where information on the rate and nature of metabolic processes is needed.

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Application of resazurin for estimating abundance of contaminant-degrading micro-organisms

Cited by 87 publications

References 13 publications

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Diamagnetic levitation enhances growth of liquid bacterial cultures by increasing oxygen availability

Microbial fuel cell technology for measurement of microbial respiration of lactate as an example of bioremediation amendment

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