Application of recombinant Sjc26GST for serodiagnosis of Schistosoma japonicum infection in water buffalo (Bos buffelus)

Cheng, Po Ching; Tsaihong, John Chin; Lee, Kin M.

doi:10.1016/j.vetpar.2007.09.022

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…Epidemiological survey has revealed that domestic animals, especially bovines, play an important role in the transmission of schistosomiasis in these regions [7]: they are the most important reservoirs for schistosomes and are considered the major source of infection for human schistiosomiasis [8]. Two kinds of bovine reared commonly in these endemic regions are yellow cattle ( Bos taurus ) and water buffalo ( Bubalus bubalis ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Yang

Feng

et al. 2012

BMC Vet Res

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BackgroundYellow cattle and water buffalo are two of the most important natural hosts for Schistosoma japonicum in China. Previous observation has revealed that yellow cattle are more suited to the development of S. japonicum than water buffalo. Understanding more about the molecular mechanisms involved in worm development, as well as the pathological and immunological differences between yellow cattle and water buffalo post infection with S japonicum will provide useful information for the vaccine design and its delivery procedure.ResultsThe worm length (p < 0.01), worm recovery rate (p < 0.01) and the percentage of paired worms (p < 0.01) were significantly greater in yellow cattle than those in water buffalo. There were many white egg granulomas in the livers of yellow cattle, but fewer were observed in water buffalo at 7 weeks post infection. The livers of infected yellow cattle contained significantly increased accumulation of inflammatory cells, and the schistosome eggs were surrounded with large amounts of eosinophil infiltration. In contrast, no hepatocyte swelling or lymphocyte infiltration, and fewer white blood cells, was observed in water buffalo. The percentage of CD4+ T cells was higher in yellow cattle, while the percentage of CD8+ T cells was higher in water buffalo from pre-infection to 7 w post infection. The CD4/CD8 ratios were decreased in both species after challenge with schistosomes. Comparing with water buffalo, the IFN-γ level was higher and decreased significantly, while the IL-4 level was lower and increased gradually in yellow cattle from pre-infection to 7 w post infection.ConclusionsIn this study, we confirmed that yellow cattle were more suited to the development of S. japonicum than water buffalo, and more serious pathological damage was observed in infected yellow cattle. Immunological analysis suggested that CD4+ T cells might be an integral component of the immune response and might associate with worm development in yellow cattle. A shift from Th1 to Th2 type polarized immunity was only shown clearly in schistosome-infected yellow cattle, but no shift in water buffalo. The results provide valuable information for increased understanding of host-schistosome interactions, and for control of schistosomiasis.

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Section: Introductionmentioning

confidence: 99%

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Yang

Feng

et al. 2012

BMC Vet Res

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“…The DNA vaccine construct was modified from pQE31/Sjc26GST, which contains the intact open reading frame (ORF) of 26 kDa SjGST from Schistosoma japonicum (GenBank accession no. BU711548.1) [ 13 ]. Briefly, the intact ORF of Sjc26GST was amplified by PCR using the pQE31/Sjc26GST vector as a template and primers containing EcoRV and BamHI restriction sites (Forward-5′-CGGGATCCCGTCATGTCCCCTATACTAGGTTAT-3′ and Reverse-5′-GGGATATCCCTTTATTTTGGAGGATGGTCGCCA-3′).…”

Section: Methodsmentioning

confidence: 99%

“…The plasmid DNA vaccine was resuspended in 5% glucose and stored at −80°C until use. In addition to pcDNA/SjGST, we used rSjGST, prepared previously ( Fig 1B ) [ 13 ], and an IL-12-encoding plasmid (pIL-12), kindly provided by the laboratory of Dr. Tauo [ 32 ], as an adjuvant to enhance the immune response.…”

Section: Methodsmentioning

confidence: 99%

“…Both native and recombinant purified Sjc26GST have been shown to provide a certain level of protection against infection, in terms of reduced worm burden, female fecundity, and egg viability [ 9 – 12 ]. We have also reported that reSjc26GST can be used for diagnosis of schistosomiasis in buffaloes, and that it provides high sensitivity and specificity [ 13 ]. In recent years, Sjc26GST has been developed into a DNA vaccine with the capacity to potentiate mainly Th1 immune responses against S .…”

Section: Introductionmentioning

confidence: 99%

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Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum

Cheng¹,

Lin²,

Peng³

et al. 2016

PLoS Negl Trop Dis

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Schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. Development of a vaccine is thought to be the most effective way to control this disease. Recombinant 26-kDa glutathione S-transferase (rSjGST) has previously been reported to achieve a worm reduction rate of 42–44%. To improve the efficiency of the vaccine against Schistosoma japonicum, we immunized mice with a combination of pcDNA vector-encoded 26-kDa SjGST (pcDNA/SjGST), IL-12 expressing-plasmid (pIL-12), and rSjGST. Co-vaccination with pcDNA/SjGST, pIL-12, and rSjGST led to a reduction in worm burden, hepatic egg burden, and the size of liver tissue granulomas than that in the untreated infection controls. In addition, we detected high levels of specific IgG, IgG1, and IgG2a against the rSjGST antigen in infected mice vaccinated with this combination of pcDNA/SjGST, pIL-12, and rSjGST. Moreover, high expression levels of Th2 cytokines, including IL-4 and IL-10, were also detected in this group, without diminished levels of IL-12, INF-γ, and TNF-α cytokines that are related to parasite killing. In conclusion, we have developed a new vaccination regimen against S. japonicum infection and shown that co-immunization with pcDNA/SjGST vaccine, pIL-12, and rSjGST has significant anti-parasite, anti-hepatic egg and anti-pathology effects in mice. The efficacy of this vaccination method should be further validated in large animals such as water buffalo. This method may help to reduce the transmission of zoonotic schistosomiasis japonica.

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“…Some defined schistosome antigens and purified recombinant antigens have been used instead of crude schistosome extracts as diagnostic antigens. These include 23 kDa membrane protein, 31/32 kDa protein, 26-kDa glutathione-S-transferase (GST), signaling protein 14–3–3, fructose bisphosphate aldolase, and 22-kDa tegumental membrane-associated antigen [ 15 – 18 ]. The use of these antigens partially improves the specificity of detection and some antigens showed potential value for evaluating the efficacy of drug treatment, but tests with most of the antigens had lower sensitivity than tests with crude worm antigens.…”

Section: Introductionmentioning

confidence: 99%

Screening Diagnostic Candidates for Schistosomiasis from Tegument Proteins of Adult Schistosoma japonicum Using an Immunoproteomic Approach

Zhang

et al. 2015

PLoS Negl Trop Dis

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BackgroundSchistosomiasis is one of the world’s most prevalent zoonotic diseases and a serious worldwide public health problem. Since the tegument (TG) of Schistosoma japonicum is in direct contact with the host and induces a host immune response against infection, the identification of immune response target molecules in the schistosome TG is crucial for screening diagnostic antigens for this disease.Methodology/Principal FindingsIn this study, an immunoproteomics approach used TG proteins as screening antigens to identify potential diagnostic molecules of S. japonicum. Ten spots corresponding to six proteins were identified that immunoreacted with sera from S. japonicum-infected rabbits but not sera from uninfected rabbits and their specific IgG antibody levels declined quickly after praziquantel treatment. Recombinant phosphoglycerate mutase (PGM) and UV excision repair protein RAD23 homolog B (RAD23) proteins were expressed and their diagnostic potential for schistosomiasis was evaluated and compared with schistosome soluble egg antigen (SEA) using ELISA. The results showed high sensitivity and specificity and low crossreactivity when rSjPGM-ELISA and rSjRAD23-ELISA were used to detect water buffalo schistosomiasis. Moreover, antibodies to rSjPGM and rSjRAD23 might be short-lived since they declined quickly after chemotherapy.Conclusion/SignificanceTherefore, the two schistosome TG proteins SjPGM and SjRAD23 were identified as potential diagnostic markers for the disease. The two recombinant proteins might have the potential to evaluate the effectiveness of drug treatments and for distinguishing between current and past infection.

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Application of recombinant Sjc26GST for serodiagnosis of Schistosoma japonicum infection in water buffalo (Bos buffelus)

Cited by 14 publications

References 29 publications

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Comparison of worm development and host immune responses in natural hosts of schistosoma japonicum, yellow cattle and water buffalo

Combined IL-12 Plasmid and Recombinant SjGST Enhance the Protective and Anti-pathology Effect of SjGST DNA Vaccine Against Schistosoma japonicum

Screening Diagnostic Candidates for Schistosomiasis from Tegument Proteins of Adult Schistosoma japonicum Using an Immunoproteomic Approach

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