Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Kimura, Katsura; Yamashita, Hiroshi; Nishimura, Tomoko; Mori, Shiho; Satoh, Yuichi

doi:10.1267/ahc.32.59

Cited by 13 publications

(3 citation statements)

References 13 publications

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“…Kimura et al (1999) were the ®rst to demonstrate that two different cell types in the intact rabbit cornea have their own individual Ca 2 mobilization response patterns when challenged with the purinergic receptor agonist ATP. Super®cial cells of the corneal epithelium produced a sustained biphasic Ca 2 transient to prolonged application of ATP, while the cells in the mid-wing layer produced synchronized Ca 2 oscillations.…”

Section: Conjunctiva and Corneamentioning

confidence: 99%

Calcium Signalling in Ocular Tissues: Functional Activity of G-protein and Tyrosine–Kinase Coupled Receptors

Duncan¹,

Collison²

2002

Experimental Eye Research

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Section: Conjunctiva and Corneamentioning

confidence: 99%

Calcium Signalling in Ocular Tissues: Functional Activity of G-protein and Tyrosine–Kinase Coupled Receptors

Duncan¹,

Collison²

2002

Experimental Eye Research

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“…Namely, cultured smooth muscle cells lose their ability to come into contact, and other alterations in their properties cannot be excluded. Consequently we concluded that the best approach is to study [Ca 2+ ] i dynamics of individual cells in intact tissue specimens using realtime confocal microscopy (Satoh et al 1997;Kimura et al 1999;Mori et al 2000;Shinohe and Saino 2000;Kumagai and Saino 2001).…”

Section: Introductionmentioning

confidence: 99%

Application of real-time confocal microscopy to intracellular calcium ion dynamics in rat arterioles

Saino

Matsuura

Satoh

2002

Histochem Cell Biol

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The regulation of cytosolic Ca(2+) homeostasis is essential for cells, including vascular smooth muscle cells. Arterial tone, which underlies the maintenance of peripheral resistance in the circulation, is a major contributor to the control of blood pressure. Confocal microscopy was employed to study the alteration in intracellular calcium ion concentration ([Ca(2+)](i)) in arterioles (external diameters <100 microm) with respect to selected modifying reagents. 5-Hydroxytryptamine (1 microM), ATP (10 microM), and endothelin 1-3 (5 nM) elicited an increase in [Ca(2+)](i) in most arteriole smooth muscle cells. The [Ca(2+)](i) increase sometimes propagated in an intercellular manner. When noradrenaline (10 microM) was used as a stimulant, [Ca(2+)](i) increase was observed only in a portion of the smooth muscle cells. It was also noted that the reaction of these cells with respect to ATP is different between testis and brain arterioles; the [Ca(2+)](i) increase in testicular arterioles is dependent on Ca(2+) influx from extracellular space, whereas in cerebral arterioles it plays a role in both the influx of extracellular Ca(2+) and the release of Ca(2+) from intracellular stores (i.e., sarco/endoplasmic reticulum). These results indicate that arterioles in different tissues may differ greatly in their responses. Real-time confocal microscopy was found to be a useful tool for investigating the structural and functional changes in living tissues.

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“…A number of studies have used cultured or isolated cells as an experimental model (Bouchelouche 1993). [Ca 2+ ] i dynamics of individual cells can be examined in intact tissue specimens using real-time confocal microscopy (Satoh et al 1997;Kimura et al 1999). [Ca 2+ ] i dynamics of individual cells can be examined in intact tissue specimens using real-time confocal microscopy (Satoh et al 1997;Kimura et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Effects of ATP on intracellular calcium dynamics of neurons and satellite cells in rat superior cervical ganglia

Kumagai

Saino

2001

Histochem Cell Biol

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Adenosine 5′-triphosphate (ATP) which is released from neuronal and non-neuronal tissues interacts with cell surface receptors to produce a broad range of physiological responses. The present study addressed the issue of whether the cells of the superior cervical ganglia (SCG) respond to ATP. To this end, the dynamics of the intracellular calcium ion concentration ([Ca 2+ ] i ) of neurons and satellite cells in intact SCG was analyzed by laser scanning confocal microscopy. ATP produced an increase of [Ca 2+ ] i in both neurons and satellite cells; initially, ATP elicited [Ca 2+ ] i increase in satellite cells and, subsequently, a [Ca 2+ ] i change in neurons was observed. P1 purinoceptor agonists had no effect on this process, but P2 purinoceptor agonists induced [Ca 2+ ] i increase and suramin totally inhibited ATP-induced [Ca 2+ ] i dynamics in both neurons and satellite cells. In satellite cells, Ca 2+ channel blockers and the removal of extracellular Ca 2+ , but not thapsigargin pretreatment, abolished ATP-induced [Ca 2+ ] i dynamics. In contrast, thapsigargin pretreatment abolished ATP-induced [Ca 2+ ] i dynamics in neurons. Reactive blue-2 inhibited the ATP-induced reaction on neurons alone. Uridine 5′-triphosphate caused a [Ca 2+ ] i increase in neurons and α,β-methylene ATP caused a [Ca 2+ ] i increase in satellite cells. We concluded that neurons respond to extracellular ATP mainly via P2Y purinoceptors and that satellite cells respond via P2X purinoceptors.

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Application of Real-Time Confocal Microscopy to Observations of ATP-Induced Ca2+-Oscillatory Fluctuations in Intact Corneal Epithlial Cells.

Cited by 13 publications

References 13 publications

Calcium Signalling in Ocular Tissues: Functional Activity of G-protein and Tyrosine–Kinase Coupled Receptors

Calcium Signalling in Ocular Tissues: Functional Activity of G-protein and Tyrosine–Kinase Coupled Receptors

Application of real-time confocal microscopy to intracellular calcium ion dynamics in rat arterioles

Effects of ATP on intracellular calcium dynamics of neurons and satellite cells in rat superior cervical ganglia

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