Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Yan, Guoquan; Du, Qian; Wei, Xuchao; Miozzi, Jackelyn; Chen, Kang; Wang, Jinnv; Han, Xinxin; Jin-huo, Pan; Xie, Hui; Chen, Jun; Zhang, Weihua

doi:10.3390/molecules23123280

Cited by 48 publications

(44 citation statements)

References 44 publications

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“…Adhering cells disrupt the interaction between the electrodes, thus impeding the electron flow. This impedance-resistance to the altering electrical current-thus corresponds to the cell number, cell morphology/size, and the strength of the cell-cell attachment onto the plate (Yan et al 2018). Figure 2a shows a dose-dependent decrease in cell viability following a 24-h incubation with MESH Classic Tobacco e-liquid and its Base liquid (with a half maximal effective concentration [EC 50 ] of 476.7 µg nicotine/mL for MESH Classic Tobacco and 423.6 µg nicotine/mL for Base e-liquid).…”

Section: First-layer Assessment: Effects Of Mesh Classic Tobacco Andmentioning

confidence: 99%

Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology

et al. 2019

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We previously proposed a systems toxicology framework for in vitro assessment of e-liquids. The framework starts with the first layer aimed at screening the potential toxicity of e-liquids, followed by the second layer aimed at investigating the toxicity-related mechanism of e-liquids, and finally, the third layer aimed at evaluating the toxicity-related mechanism of the corresponding aerosols. In this work, we applied this framework to assess the impact of the e-liquid MESH Classic Tobacco and its aerosol compared with that of cigarette smoke (CS) from the 3R4F reference cigarette. In the first layer, we evaluated the cytotoxicity profile of the MESH Classic Tobacco e-liquid (containing humectants, nicotine, and flavors) and its Base e-liquid (containing humectant and nicotine only) in comparison with total particulate matter (TPM) of 3R4F CS using primary bronchial epithelial cell cultures. In the second layer, the same culture model was used to explore changes in specific markers using high-content screening assays to identify potential toxicity-related mechanisms induced by the MESH Classic Tobacco and Base e-liquids beyond cell viability in comparison with the 3R4F CS TPM-induced effects. Finally, in the third layer, we compared the impact of exposure to the MESH Classic Tobacco or Base aerosols with 3R4F CS using human organotypic air-liquid interface buccal and small airway epithelial cultures. The results showed that the cytotoxicity of the MESH Classic Tobacco liquid was similar to the Base liquid but lower than 3R4F CS TPM at comparable nicotine concentrations. Relative to 3R4F CS exposure, MESH Classic Tobacco aerosol exposure did not cause tissue damage and elicited lower changes in the mRNA, microRNA, and protein markers. In the context of tobacco harm reduction strategy, the framework is suitable to assess the potential-reduced impact of electronic cigarette aerosol relative to CS.

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Section: First-layer Assessment: Effects Of Mesh Classic Tobacco Andmentioning

confidence: 99%

Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology

et al. 2019

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“…Mast cell degranulation was evaluated according to real-time monitoring of cellular morphology using an RTCA TP instrument [23]. The cell index (CI) value as read on the machine directly responds to the cell attaching properties, which vary when degranulation occurs [24,25]. According to the CI curve, forsythoside A and forsythoside B could stimulate mast cell degranulation.…”

Section: Forsythoside a And Forsythoside B Slightly Evoke Mast Cell Dmentioning

confidence: 99%

“…Assessment of mast cell degranulation was performed using the xCELLigence Real Time Cellular Analysis (RTCA) TP instrument (ACEA Biosciences, CA, USA) [24,25]. RBL-2H3 mast cells were seeded in E-16 plates (5×10 3 cells/well).…”

Section: Assessment Of Mast Cell Degranulationmentioning

confidence: 99%

Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway

Han

Zhang

Pan

et al. 2019

IJMS

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In recent years, hypersensitivity reactions to the Shuanghuanglian injection have attracted broad attention. However, the componential chief culprits inducing the reactions and the underlying mechanisms involved have not been completely defined. In this study, we used a combination of approaches based on the mouse model, human umbilical vein endothelial cell monolayer, real-time cellular monitoring, immunoblot analysis, pharmacological inhibition, and molecular docking. We demonstrated that forsythoside A and forsythoside B contributed to Shuanghuanglian injection-induced pseudoallergic reactions through activation of the RhoA/ROCK signaling pathway. Forsythoside A and forsythoside B could trigger dose-dependent vascular leakage in mice. Moreover, forsythoside A and forsythoside B slightly elicited mast cell degranulation. Correspondingly, treatment with forsythoside A and forsythoside B disrupted the endothelial barrier and augmented the expression of GTP-RhoA, p-MYPT1, and p-MLC2 in a concentration-dependent manner. Additionally, the ROCK inhibitor effectively alleviated forsythoside A/forsythoside B-induced hyperpermeability in both the endothelial cells and mice. Similar responses were not observed in the forsythoside E-treated animals and cells. These differences may be related to the potential of the tested compounds to react with RhoA-GTPγS and form stable interactions. This study innovatively revealed that some forsythosides may cause vascular leakage, and therefore, limiting their contents in injections should be considered.

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“…It is mainly helping to cure cervical and lung carcinomas [35][36][37][38][39][40] . Not only anti-MiRs or nanoparticles are used in cancer treatment, but also the use of DNA methylation has been increased to understand the cases of somatic cell reprogramming and tumorigenesis [41][42][43][44][45][46][47][48][49][50][51] .…”

Section: Introductionmentioning

confidence: 99%

Insight into DNA Methylation

Shu

2019

J. Drug Delivery Ther.

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Application of Real-Time Cell Electronic Analysis System in Modern Pharmaceutical Evaluation and Analysis

Cited by 48 publications

References 44 publications

Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology

Application of a multi-layer systems toxicology framework for in vitro assessment of the biological effects of Classic Tobacco e-liquid and its corresponding aerosol using an e-cigarette device with MESH™ technology

Forsythoside A and Forsythoside B Contribute to Shuanghuanglian Injection-Induced Pseudoallergic Reactions through the RhoA/ROCK Signaling Pathway

Insight into DNA Methylation

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