Application of Raman Spectroscopy to Study the Mineralization of Bone Regenerates

Abstract

Assessment of the state of bone tissue during biopsy examination of the material of patients with degenerative or traumatic diseases is a very urgent task of modern medicine. Such an assessment makes it possible to characterize the regenerative processes at the site of the bone defect and to choose the most effective treatment strategies. Currently, in the study of bone tissue, methods of X-ray examination and survey histological staining are used. Such methods do not allow a highly sensitive analysis of individual components of the cellular substance. One approach to solving this problem can be the use of Raman spectroscopy. In contrast to infrared spectroscopy, Raman spectroscopy records in the optical range, which ensures high sensitivity and ease of use. Raman spectroscopy is a non-contact, non-destructive testing method suitable for working with substances in any phase. In this scientific work, a study of the distribution of hydroxyapatite in a section of the regenerative material of the parietal bone of the rat skull is carried out. As a result, it was shown that the Raman spectroscopy method can be used to determine the characteristics of the formation, mineralization, and maturation of bone tissue, to identify loci with maximum and minimum mineralization.