Application of Raman Spectroscopy in Biomedical Diagnostics

Mhlanga, Nikiwe; Tetyana, Phumlani; Nyembe, Sanele; Sikhwivhilu, Lucky

doi:10.5772/intechopen.99771

Cited by 3 publications

(1 citation statement)

References 148 publications

(188 reference statements)

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“…Raman spectroscopy could also serve as a fast, reliable, reproducible, non-invasive, non-destructive, cost-effective and environmentally friendly technique, requiring only a few seconds or minutes for sample preparation, in contrast to HPLC [ 17 ]. Thus, the applications of Raman spectroscopy in pharmaceutical and biomedical sciences are impressively increasing [ 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Analytical Method for the Quantitation of Monoclonal Antibodies Solutions via Raman Spectroscopy: The Case of Bevacizumab

Lykouras,

Papaspyridakou,

Makri

et al. 2024

Pharmaceuticals

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Personalized dosages of monoclonal antibodies are being used more regularly to treat various diseases, rendering their quantitation more essential than ever for the right dose administration to the patients. A promising alternative, which overcomes the obstacles of the well-established chromatographic techniques regarding the quantification of biopharmaceuticals, is Raman spectroscopy. This study aimed to develop and validate a novel analytical method for the quantitation of bevacizumab in solutions via Raman spectroscopy. For this purpose, a droplet of the solution was left to dry on a highly reflective carrier and a home-made apparatus was employed for rotation of the sample. Hence, each recorded Raman spectrum was the average of the signal acquired simultaneously from multiple points on a circular circumference. The method was validated, and the detection limit of the antibody was found to be 1.06 mg/mL. Bevacizumab was found to be highly distributed at the formed coffee ring of the dried droplet, though this was a function of solution concentration. Finally, Raman spectra at different distances on the coffee ring were obtained from the four quarters. The lowest bevacizumab detection limit was found at a distance of 75 μm from the external side of the coffee ring and it was determined to be equal to 0.53 mg/mL.

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Section: Introductionmentioning

confidence: 99%

Development of Analytical Method for the Quantitation of Monoclonal Antibodies Solutions via Raman Spectroscopy: The Case of Bevacizumab

Lykouras,

Papaspyridakou,

Makri

et al. 2024

Pharmaceuticals

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SERS liquid biopsy in breast cancer. What can we learn from SERS on serum and urine?

Iancu

Cozan

Ştefancu

et al. 2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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An all metasurface-based fiber needle probe for Raman spectroscopy

Zhang

Bi²,

Zhang³

et al. 2022

Front. Phys.

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Optical fiber-based spectroscopy sensors are widely used for industrial and biomedical applications. They normally consist of at least one excitation fiber and one collection fiber. However, the excitation and collection fibers are placed side by side, so the focal spots do not coincide. In addition, Raman probes whose excitation and emission span a wide wavelength range are limited by wavelength-dependent focal length variation, low sensitivity, and bulky size impeding their clinical adoption. To overcome the challenges, we propose an all metasurface integrated fiber solution. The metasurface technology is well suited for this application because it relies on specially designed nanostructures to manipulate light properties in an ultrathin footprint. Here we used our earlier demonstrated dual-wavelength excitation Raman probe as an example. The two excitation fibers at 671 nm and 785 nm feature a hybrid metasurface lens (metalens) including a narrow band pass filter and an off-axis focusing metalens. The collection fiber at 810—910 nm features an achromatic broadband on-axis focusing metalens. Simulation results show that by integrating the metalenses, the focal spots of the excitation beams and the collection beam coincide with a beam size of 4.6 µm, 4.6 µm, and 11.3 µm in the x-, y-, and z-axis, respectively. Moreover, the probe size shrinks by 100 times and becomes a needle probe. The needle probe will enable new applications such as small animal in vivo experiments, medical endoscopy experiments, and neonatal skin analysis for hard-to-reach areas. Furthermore, the proposed solution can be applied to work with any optical fiber-based spectroscopy sensors because the designs can be readily fabricated and put into practical use.

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Application of Raman Spectroscopy in Biomedical Diagnostics

Cited by 3 publications

References 148 publications

Development of Analytical Method for the Quantitation of Monoclonal Antibodies Solutions via Raman Spectroscopy: The Case of Bevacizumab

Development of Analytical Method for the Quantitation of Monoclonal Antibodies Solutions via Raman Spectroscopy: The Case of Bevacizumab

SERS liquid biopsy in breast cancer. What can we learn from SERS on serum and urine?

An all metasurface-based fiber needle probe for Raman spectroscopy

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