Application of Proteomics to the Study of Cardiovascular Biology

Macri, Joseph; Rapundalo, Stephen T.

doi:10.1016/s1050-1738(01)00088-3

Cited by 50 publications

(21 citation statements)

References 94 publications

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“…Several excellent reviews on various aspects of proteomic techniques are available [29][30][31][32][33] and will therefore be discussed here only briefly. With advancement in mass spectrometry (MS), bioinformatics, and analytical methods, proteomic approaches greatly promote serum protein analysis and biomarker discovery ( Figure 2).…”

Section: Technologies Involved In Proteomicsmentioning

confidence: 99%

“…Though cancer is asymptomatic in its early stage, autoantibodies produced in response to cancer-specific antigens may be detectable due to signal amplification from the humoral immune response. To date, many autoantibody targets have been evaluated for their value in early detection as well as prognosis of lung cancer [12,15,18,[26][27][28][29][30][31]. However, despite many leads, little has been done to validate these findings and to translate these promising antibodies into clinically usable tests.…”

Section: Potential Of Lung Cancer Protein Biomarkersmentioning

confidence: 99%

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Serum-based protein biomarkers for detection of lung cancer

2014

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Lung cancer is one of the most common cancers in terms of both incidence and mortality.The major reasons for the increasing number of deaths from lung cancer are late detection and lack of effective therapies. To improve our understanding of lung cancer biology, there is urgent need for blood-based, non-invasive molecular tests to assist in its detection in a cost-effective manner at an early stage when curative interventions are still possible. Recent advances in proteomic technology have provided extensive, high throughput analytical tools for identification, characterization and functional studies of proteomes. Changes in protein expression patterns in response to stimuli can serve as indicators or biomarkers of biological and pathological processes as well as physiological and pharmacological responses to drug treatment, thus aiding in early diagnosis and prognosis of disease. However, only a few biomarkers have been approved by the FDA to date for screening and diagnostic purposes. This review provides a brief overview of currently available proteomic techniques, their applications and limitations and the current state of knowledge about important serum biomarkers in lung cancer and their potential value as prognostic and diagnostic tools.

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Section: Technologies Involved In Proteomicsmentioning

confidence: 99%

Section: Potential Of Lung Cancer Protein Biomarkersmentioning

confidence: 99%

Serum-based protein biomarkers for detection of lung cancer

2014

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“…Recent developments in proteomics have permitted robust investigations into the molecular mechanisms underlying cardiovascular disease and have allowed for identification of protein modi-fications (Arrell et al, 2001). Proteomics techniques also offer the ability to simultaneously monitor differential changes in protein expression in response to various stimuli and under different physiological and pathological conditions (Macri and Rapundalo, 2001). In previous studies, proteomics approaches have been used to identify DOX-induced protein changes in MCF-7 human breast cancer cells (Chen et al, 2002).…”

mentioning

confidence: 99%

Modulation of Cytochrome c Oxidase-Va Is Possibly Involved in Metallothionein Protection from Doxorubicin Cardiotoxicity

Merten¹,

Feng²,

Zhang³

et al. 2005

J Pharmacol Exp Ther

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Previous studies using a cardiac-specific metallothionein (MT)-overexpressing transgenic (MT-TG) mouse model have demonstrated that MT protects from doxorubicin (DOX)-induced oxidative heart injury. The molecular mechanisms that underlie this cardioprotection, however, have yet to be defined. In the present study, we tested the hypothesis that MT overexpression activates cytoprotective mechanisms, leading to cardiac protection from DOX toxicity. MT-TG mice and nontransgenic wild-type (WT) controls were treated i.p. with DOX at a single dose of 20 mg/kg and sacrificed on the third day after the treatment. An expression proteomic analysis involving twodimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight mass spectrometry was used to identify MT-induced changes in cytoprotection-related proteins. We identified 18 proteins that were modified by DOX treatment in the heart. These proteins included those involved in cellular antioxidant defense, enzymes of the mitochondrial electron transport chain, enzymes involved in ␤-oxidation of fatty acids and glycolysis, and proteins involved in regulation of cardiac muscle contraction. However, the most dominant modification by MT is the cytochrome c oxidase subunit Va (CCOVa). In response to DOX treatment, a specific isoform of CCO-Va was enhanced in the MT-TG but not in the WT mouse hearts. Because CCO-Va is a critical component in the mitochondrial electron transport chain, the results suggest that the cardioprotective effect of MT may be related to an increased expression or a differential modification of CCO-Va.

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“…Also, DNA acts like a blueprint of the cell, whereas proteins are the dynamic components. Analysis of human liver indicated that the correlation coefficient between the amount of mRNA and the abundance of corresponding proteins is only 0.48, which further demonstrates the importance of proteomic studies [7]. In the present study, a proteomebased approach involving 2D-DIGE was used to analyze differences in protein expression for stage 4 neuroblastoma, stage 4s neuroblastoma, and normal adrenal tissue adjacent to the tumors.…”

mentioning

confidence: 75%