Application of proteomics in studying bacterial persistence

Sulaiman, Jordy Evan; Lam, Henry

doi:10.1080/14789450.2019.1575207

Cited by 17 publications

(22 citation statements)

References 95 publications

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“…It may be because the present models fail to capture some of the "hidden" costs associated with tolerance in the hostile environment of the host (Van den Bergh et al, 2017). Additional factors such as host defense and species competition may also come into play in vivo (Sakoulas et al, 2017;Sulaiman and Lam, 2019). Although models for in vivo evolution are still lacking, researchers have been performing longitudinal studies of bacteria strains isolated from patients, thereby revealing the dynamics of tolerance evolution within the host (Liu et al, 2020).…”

Section: Future Directions In Studying Evolution Of Bacterial Tolerancementioning

confidence: 97%

“…The presence of persisters explains the biphasic killing pattern when bacteria are treated with bactericidal antibiotics. The first phase with the steeper slope marks the rapid decline of the susceptible cells, and the second phase indicates the slow decline of the persister cells (Sulaiman and Lam, 2019).…”

Section: Distinction Between Bacterial Tolerance and Resistance As Sumentioning

confidence: 99%

“…The tolerance mutations detected in laboratory evolution experiments across different laboratories are different and seemingly unrelated, perhaps due to the slight variations in the experimental conditions, the different ancestral strains used for the evolution experiments (with slight mutational variations), and/or the random nature of mutagenesis (Sulaiman and Lam, 2019). The mutated genes were involved in many and varied cellular functions.…”

Section: Tolerance and Resistance Mutations Identified From Laboratormentioning

confidence: 99%

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Evolution of Bacterial Tolerance Under Antibiotic Treatment and Its Implications on the Development of Resistance

Sulaiman

Lam

2021

Front. Microbiol.

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Recent laboratory evolution studies have shown that upon repetitive antibiotic treatments, bacterial populations will adapt and eventually became tolerant and resistant to the drug. Drug tolerance rapidly evolves upon frequent, intermittent antibiotic treatments, and such emerging drug tolerance seems to be specific to the treatment conditions, complicating clinical practice. Moreover, it has been shown that tolerance often promotes the development of resistance, which further reinforces the need of clinical diagnostics for antibiotic tolerance to reduce the occurrence of acquired resistance. Here, we discuss the laboratory evolution studies that were performed to track the development of tolerance in bacterial populations, and highlight the urgency of developing a comprehensive knowledge base of various tolerance phenotypes and their detection in clinics. Finally, we propose future directions for basic research in this growing field.

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Section: Future Directions In Studying Evolution Of Bacterial Tolerancementioning

confidence: 97%

Section: Distinction Between Bacterial Tolerance and Resistance As Sumentioning

confidence: 99%

Section: Tolerance and Resistance Mutations Identified From Laboratormentioning

confidence: 99%

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Evolution of Bacterial Tolerance Under Antibiotic Treatment and Its Implications on the Development of Resistance

Sulaiman

Lam

2021

Front. Microbiol.

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“…Some of these biological components also showed a high similarity with those involved in pathogenesis and resistance mechanisms in the opportunistic pathogen P. aeruginosa (Quintieri et al, 2019b), although their role in food spoilage pseudomonads is not yet completely clear. In the study of bacterial persistence, the application of proteomic techniques and the development of information-rich databases have been helping to reveal metabolic pathways involved in a specific physiological state and to provide direct information on druggable targets (Sulaiman and Lam, 2019). Thus, comparative proteomic under treatments with novel antibiofilm molecules (Rasamiravaka et al, 2015;Chan et al, 2016;Caputo et al, 2018;Li et al, 2018;Ahmed et al, 2019) is a high-throughput tool to reveal major putative targets that are directly or indirectly involved in the expression phenotype by different microorganisms (Chatrath et al, 2018;Qayyum et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Biofilm and Pathogenesis-Related Proteins in the Foodborne P. fluorescens ITEM 17298 With Distinctive Phenotypes During Cold Storage

et al. 2020

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In food chain, Pseudomonas spp. cause spoilage by reducing shelf life of fresh products, especially during cold storage, with a high economic burden for industries. However, recent studies have shed new light on health risks occurring when they colonize immunocompromised patient tissues. Likewise to P. aeruginosa, they exhibit antibiotic resistance and biofilm formation, responsible for their spread and persistence in the environment. Biofilm formation might be induced by environmental stresses, such as temperature fluctuations causing physiological and metabolic changes exacerbating food spoilage (by protease and pigment synthesis), and the production of adhesion molecules, chemotactic or underestimated virulence factors. In order to provide a new insight into phenotypic biodiversity of Pseudomonas spoilers isolated from cold stored cheese, in this work 19 Pseudomonas spp. were investigated for biofilm, pigments, exopolysaccharide production and motility at low temperature. Only nine strains showed these phenotypic traits and the blue pigmenting cheese strain P. fluorescens ITEM 17298 was the most distinctive. In addition, this strain decreased the survival probability of infected Galleria mellonella larvae, showing, for the first time, a pathogenic potential. Genomic and proteomic analyses performed on the ITEM 17298 planktonic cells treated or not with lactoferrin derived antibiofilm peptides allowed to reveal specific biofilm related-pathways as well as proteins involved in pathogenesis. Indeed, several genes were found related to signaling system by cGMP-dependent protein kinases, cellulose, rhamnolipid and alginate synthesis, antibiotic resistance, adhesion and virulence factors. The proteome of the untreated ITEM 17298, growing at low temperature, showed that most of the proteins associated with biofilm regulation, pigmentation motility, antibiotic resistance and pathogenecity were repressed, or decreased their levels in comparison to that of the untreated cultures. Thus, the results of this work shed light on the complex pathways network allowing psychrotrophic pseudomonads to adapt themselves to

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“…Proteomic analyses have a wide range of applications. Examples include investigating different protein levels in resistant [ 30 ] and drug-tolerant bacterial phenotypes [ 31 ] ( Table 2 ), as well as reports on the bacterial adaptation to different growing conditions and bacterial catabolism [ 32 ], biomarker discovery [ 33 ], and pathogen-host cell interactions [ 34 , 35 ]. In several other studies, different proteome analysis approaches have been employed to study the bacterial response to commonly used antibiotics such as ciprofloxacin [ 36 , 37 , 38 , 39 , 40 ], tobramycin [ 41 , 42 ], colistin [ 43 , 44 , 45 ], polymyxin B [ 46 ], daptomycin [ 47 , 48 ], and silver nanoparticles [ 49 ] ( Table 2 ).…”

Section: Introductionmentioning

confidence: 99%

The Role of Proteomics in Bacterial Response to Antibiotics

et al. 2020

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For many years, we have tried to use antibiotics to eliminate the persistence of pathogenic bacteria. However, these infectious agents can recover from antibiotic challenges through various mechanisms, including drug resistance and antibiotic tolerance, and continue to pose a global threat to human health. To design more efficient treatments against bacterial infections, detailed knowledge about the bacterial response to the commonly used antibiotics is required. Proteomics is a well-suited and powerful tool to study molecular response to antimicrobial compounds. Bacterial response profiling from system-level investigations could increase our understanding of bacterial adaptation, the mechanisms behind antibiotic resistance and tolerance development. In this review, we aim to provide an overview of bacterial response to the most common antibiotics with a focus on the identification of dynamic proteome responses, and through published studies, to elucidate the formation mechanism of resistant and tolerant bacterial phenotypes.

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Application of proteomics in studying bacterial persistence

Cited by 17 publications

References 95 publications

Evolution of Bacterial Tolerance Under Antibiotic Treatment and Its Implications on the Development of Resistance

Evolution of Bacterial Tolerance Under Antibiotic Treatment and Its Implications on the Development of Resistance

Biofilm and Pathogenesis-Related Proteins in the Foodborne P. fluorescens ITEM 17298 With Distinctive Phenotypes During Cold Storage

The Role of Proteomics in Bacterial Response to Antibiotics

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