Application of Probiotics from Mongolian Dairy Products to Fermented Dairy Products and Its Effects on Human Defecation

Takeda, Shiro; Fujimoto, Ryoji; Takenoyama, Shin-ichi; Takeshita, Masahiko; Kikuchi, Yukiharu; Tsend-Ayush, Chuluunbat; Dashnyam, Bumbein; Muguruma, Michio; Kawahara, Satoshi

doi:10.3136/fstr.19.245

Cited by 8 publications

(7 citation statements)

References 28 publications

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“…(2011) suggested that lactic acid bacteria are widely distributed in natural fermented foods as indigenous microflora. Also previous studies have been performed to analyze the diversity of the microbial species existing in traditional fermented dairy products in various locations, such as Turkey ( Gurses and Erdogan, 2006 ), Africa ( Mathara et al ., 2004 ), Italy ( Losio et al ., 2014 ), Mongolia ( Takeda et al ., 2013 ), Iran ( Azadnia and Khan Nazer, 2009 ), Morocco ( Ouadghiri et al ., 200 9) etc.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species

Wang¹,

Liu²,

Ren³

et al. 2016

Korean Journal for Food Science of Animal Resources

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In this study, traditional culture method and 16S rRNA gene analysis were applied to reveal the composition and diversity of lactic acid bacteria (LAB) of fermented cow milk, huruud and urum from Baotou and Bayannur of midwestern Inner Mongolia. Also, the quantitative results of dominant LAB species in three different types of dairy products from Baotou and Bayannur were gained by quantitative polymerase chain reaction (q-PCR) technology. Two hundred and two LAB strains isolated from sixty-six samples were identified and classified into four genera, namely Enterococcus, Lactococcus, Lactobacillus, Leuconostoc, and twenty-one species and subspecies. From these isolates, Lactococcus lactis subsp. lactis (32.18%), Lactobacillus plantarum (12.38%) and Leuconosto mesenteroides (11.39%) were considered as the dominated LAB species under the condition of cultivating in MRS and M17 medium. And the q-PCR results revealed that the number of dominant species varied from samples to samples and from region to region. This study clearly shows the composition and diversity of LAB existing in fermented cow milk, huruud and urum, which could be considered as valuable resources for LAB isolation and further probiotic selection.

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Section: Introductionmentioning

confidence: 99%

Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species

Wang¹,

Liu²,

Ren³

et al. 2016

Korean Journal for Food Science of Animal Resources

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“…DNA extraction from fecal samples and intestinal bacteria assay. For quantification by quantitative PCR, DNA was extracted from fecal samples obtained from 06cc2-treated and control mice on day 20 using an iSoFecal for Beads Beating kit (Nippon Gene) according to the manufacturer's instructions as described in a previous report (20). dna was also extracted from standard strains for each group, including Bifidobacterium longum JcM 1217 for the Bifidobacterium subspecies, the 06Tca19 strain for the Lactobacillus group, Clostridium coccoides JcM 1395 for the Clostridium group, and Bacteroides fragilis JcM 11019 for the Bacteroides-Prevotella group.…”

Section: Cell Separation Of Lpmcs By Magnetic-activated Cell Sorting mentioning

confidence: 99%

“…Bifidobacterium subspecies, C. coccoides, Bacteroides-Prevotella, and Lactobacillus groups were detected by SYBr-Green quantitative PCR. Specific primers are shown in Table II (20). Bacterial CFUs were calculated using a standard regression curve of the threshold cycle values generated from 10-fold serial dilutions of DNA samples from the standard strains with known numbers of cFus.…”

Section: Cell Separation Of Lpmcs By Magnetic-activated Cell Sorting mentioning

confidence: 99%

Oral administration of Lactobacillus�plantarum 06CC2 prevents experimental colitis in mice via an anti‑inflammatory response

et al. 2020

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dysbiosis of the enteric microbiota causes gastrointestinal diseases, including colitis. The present study investigated the beneficial effect of Lactobacillus plantarum 06cc2 in experimental colitis in mice. an experimental colitis model in c57Bl6 mice was induced using dextran sulfate sodium. Mice were orally administered 06cc2 (06cc2 group) or PBS only (control group) by gavage. The disease activity index (dai), histological grading, and colon tissue and colonic lamina propria mononuclear cells (lPMcs) were examined macroscopically and histopathologically, and the expression levels of inflammation-associated cytokines (il-6, il-12, TnF-α and il-10) in these samples were determined. compared with the control group, the 06cc2 group exhibited a significantly lower dai (1.5±0.8 vs. 0.2±0.3, respectively; P<0.05) and pathology score (6.3±1.5 vs. 3.8±1.3, respectively; P<0.05). il-10 expression in colonic lPMcs was higher in the 06cc2 group than in the control group, although there was no significant difference in IFN-γ, il-6 or il-12 expression in colonic lPMcs between the two groups. in addition, 06cc2 stimulated the production of il-10 from cd11b-positive cells and cd11c-positive cells in the colon. The 06cc2 strain induced il-10 production in the colon and attenuated colon inflammation.

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“…Among the isolates, Lactobacillus (L.) paracasei strain 06TCa19 improved human defecation and showed potential as a probiotic [22]. Furthermore, strain 06TCa19 demonstrated anti-H. pylori activity in vitro and in vivo [23].…”

Section: Introductionmentioning

confidence: 96%

Lactobacillus paracasei strain 06TCa19 suppresses inflammatory chemokine induced by Helicobacter pylori in human gastric epithelial cells

et al. 2017

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Helicobacter (H.) pylori infection is an important risk factor for gastric cancer that causes gastric inflammation. Inflammatory chemokines such as interleukin (IL)-8 and regulated on activation normal T cell expressed and secreted (RANTES) are elevated in the gastric mucosa by H. pylori. This study aimed to investigate the effects of Lactobacillus paracasei strain 06TCa19, a probiotic strain, on IL-8 and RANTES expression and production induced by H. pylori using human gastric epithelial cell lines. Strain 06TCa19 was shown to suppress H. pylori-mediated elevation of gene expression related to these chemokines in MKN45 cells. The strain also suppressed the increase in IL-8 and RANTES products induced by H. pylori in AGS cells as well as in MKN45 cells. In MKN45 cells inoculated with H. pylori, strain 06TCa19 was shown to downregulate the activation of NF-κB and p38 MAPK signaling pathways. Additionally, the level of the CagA virulence protein of H. pylori in the MKN45 cells and the number of viable H. pylori adhering to MKN45 cells decreased with the addition of strain 06TCa19. Moreover, the strain 06TCa19 notably increased lactic acid in the supernatant of MKN45 cells. Thus, lactic acid released from strain 06TCa19 might have inhibited the adhesion of H. pylori to MKN45 cells and prevented the insertion of H. pylori CagA into the cells, and elevation of IL-8 and RANTES genes and proteins might be suppressed by downregulating the NF-κB and p38 MAPK pathways. Therefore, use of strain 06TCa19 may prevent H. pylori-associated gastric inflammation.

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Application of Probiotics from Mongolian Dairy Products to Fermented Dairy Products and Its Effects on Human Defecation

Cited by 8 publications

References 28 publications

Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species

Isolation and Identification of Lactic Acid Bacteria from Traditional Dairy Products in Baotou and Bayannur of Midwestern Inner Mongolia and q-PCR Analysis of Predominant Species

Oral administration of Lactobacillus�plantarum 06CC2 prevents experimental colitis in mice via an anti‑inflammatory response

Lactobacillus paracasei strain 06TCa19 suppresses inflammatory chemokine induced by Helicobacter pylori in human gastric epithelial cells

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