Application of potentiometric biosensor based on recombinant urease for urea determination in blood serum and hemodialyzate

Марченко, С. В.; Кучеренко, І. С.; Hereshko, A.N.; Panasiuk, I.V.; Солдаткін, О. О.; El'skaya, A. V.; Солдаткин, А. П.

doi:10.1016/j.snb.2014.06.136

Cited by 45 publications

(19 citation statements)

References 27 publications

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“…Another common method for determining the electrolyte concentrations in the liquid phase is electrochemical potentiometric measurement using ISEs or ion-selective fieldeffect transistors (ISEFETs). For this, a concentrationdependent voltage is measured between the ISEs and a reference electrode (Mikhelson, 2013;Cammann, 1979). Schindler et al developed a flow cell for ISEs (Schindler and Schindler, 1983;Schindler and Glich, 1981).…”

Section: State Of the Art And Conceptmentioning

confidence: 99%

Continuous in-line monitoring of electrolyte concentrations in extracorporeal circuits for individualization of dialysis treatment

Berger

Faulstich

Perl

et al. 2018

J. Sens. Sens. Syst.

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Abstract. One objective of dialysis treatment is to normalize the blood plasma electrolytes and remove waste products such as urea and creatinine from blood. However, due to a shift in plasma osmolarity, a rapid or excessive change of the electrolytes can lead to complications like cardiovascular instability, overhydrating of cells, disequilibrium syndrome and cardiac arrhythmias. Especially for critical ill patients in intensive care unit with sepsis or multi-organ failure, any additional stress has to be avoided. Since the exchange velocity of the electrolytes mainly depends on the concentration gradients across the dialysis membrane between blood and dialysate, it can be controlled by an individualized composition of dialysate concentrations. In order to obtain a precise concentration gradient with the individualized dialysate, it is necessary to continuously monitor the plasma concentrations. However, with in-line sensors, the required hemocompatibility is often difficult to achieve. In this work, we present a concept for continuous in-line monitoring of electrolyte concentrations using ion-selective electrodes separated from the blood flow by a dialysis membrane, and therefore meeting the fluidic requirements for hemocompatibility. First investigations of hemocompatibility with reconfigured human blood show no increased hemolysis caused by the measuring system. With this concept, it is possible to continuously measure the plasma concentrations with a relative error of less than 0.5 %.

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Section: State Of the Art And Conceptmentioning

confidence: 99%

Continuous in-line monitoring of electrolyte concentrations in extracorporeal circuits for individualization of dialysis treatment

Berger

Faulstich

Perl

et al. 2018

J. Sens. Sens. Syst.

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“…Furthermore, the urea level in cattle milk is a useful parameter to control animal health, and levels above 40 mg dL −1 (6.6 m m ) can be indicative of a disease . In the last three years, the development of simple methodologies for urea detection has attracted considerable attention and several studies have been reported (see Table SI‐1, Supporting Information), most of them based on electrochemical sensing –. Although previous electrochemical sensors have generally required two enzymes (urease and glutamate dehydrogenase) and mediators [e.g., reduced nicotinamide adenine dinucleotide (NADH), l ‐glutamate, ferrocene] to complete electron transfer to the electrode, recent studies have aimed to reduce complexity and the number of chemicals needed by using polymer composites and graphene‐modified electrodes –.…”

Section: Introductionmentioning

confidence: 99%

“…In the last three years, the development of simple methodologies for urea detection has attracted considerable attention and several studies have been reported (see Table SI‐1, Supporting Information), most of them based on electrochemical sensing –. Although previous electrochemical sensors have generally required two enzymes (urease and glutamate dehydrogenase) and mediators [e.g., reduced nicotinamide adenine dinucleotide (NADH), l ‐glutamate, ferrocene] to complete electron transfer to the electrode, recent studies have aimed to reduce complexity and the number of chemicals needed by using polymer composites and graphene‐modified electrodes –. Furthermore, a colorimetric method based on the aggregation of unmodified gold nanoparticles with a detection limit of 20 m m , as well as a urea probe based on the magnetization of iron nanoparticles in oil‐in‐water emulsions have been reported .…”

Section: Introductionmentioning

confidence: 99%

A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme‐Mediated Detachment of Labeled Reporters: The Example of Urea Detection

Llopis‐Lorente

Villalonga

Marcos

et al. 2018

Chemistry A European J

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Here, a new bio‐inspired nanoarchitectonics approach for the design of optical probes is presented. It is based on nanodevices that combine 1) an enzymatic receptor subunit, 2) a signaling subunit (consisting of a labeled reporter attached to a silica surface), and 3) a mechanism of communication between the two sites based on the production of chemical messengers by the enzymatic subunit, which induces the detachment of the reporter molecules from the silica surface. As a proof of concept, a urea nanosensor based on the release of Alexa‐Fluor‐647‐labeled oligonucleotide from enzyme‐functionalized Janus gold–mesoporous‐silica nanoparticles (Au–MSNPs) was developed. The Janus particles were functionalized on the silica face with amino groups to which the labeled oligonucleotides were attached by electrostatic interactions, whereas the gold face was used for grafting urease enzymes. The nanodevice was able to release the fluorescent oligonucleotide through the enzyme‐mediated hydrolysis of urea to ammonia and the subsequent deprotonation of amino groups on the silica face. This simple nanodevice was applied for the fluorometric detection of urea in real human blood samples and for the identification of adulterated milk. Given the large variety of enzymes and reporter species that could be combined, this is a general new paradigm that could be applied to the design of a number of optical probes for the detection of target analytes.

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“…Electrochemical urea biosensors including amperometric, 1,2 potentiometric, 3,4 and field effect 5 sensors have been extensively investigated and reported due to their simplicity. Colorimetric methods have also been explored as a simple and inexpensive alternative.…”

Section: Introductionmentioning

confidence: 99%

Remote calorimetric detection of urea via flow injection analysis

Gaddes

Demirel

Reeves

et al. 2015

Analyst

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The design and development of a calorimetric biosensing system enabling relatively high throughput sample analysis are reported. The calorimetric biosensor system consists of a thin (~20 μm) micromachined Y-cut quartz crystal resonator (QCR) as a temperature sensor placed in close proximity to a fluidic chamber packed with an immobilized enzyme. Layer by layer enzyme immobilization of urease is demonstrated and its activity as a function of the number of layers, pH, and time has been evaluated. This configuration enables a sensing system where a transducer element is physically separated from the analyte solution of interest and is thereby free from fouling effects typically associated with biochemical reactions occuring on the sensor surface. The performance of this biosensing system is demonstrated by detection of 1–200 mM urea in phosphate buffer via a flow injection analysis (FIA) technique. Miniaturized fluidic systems were used to provide continuous flow through a reaction column. Under this configuration the biosensor has an ultimate resolution of less than 1 mM urea and showed a linear response between 0–50 mM. This work demonstrates a sensing modality in which the sensor itself is not fouled or contaminated by the solution of interest and the enzyme immobilized Kapton® fluidic reaction column can be used as a disposable cartridge. Such a system enables reuse and reliability for long term sampling measurements. Based on this concept a biosensing system is envisioned which can perform rapid measurements to detect biomarkers such as glucose, creatinine, cholesterol, urea and lactate in urine and blood continuously over extended periods of time.

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Application of potentiometric biosensor based on recombinant urease for urea determination in blood serum and hemodialyzate

Cited by 45 publications

References 27 publications

Continuous in-line monitoring of electrolyte concentrations in extracorporeal circuits for individualization of dialysis treatment

Continuous in-line monitoring of electrolyte concentrations in extracorporeal circuits for individualization of dialysis treatment

A Versatile New Paradigm for the Design of Optical Nanosensors Based on Enzyme‐Mediated Detachment of Labeled Reporters: The Example of Urea Detection

Remote calorimetric detection of urea via flow injection analysis

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