Application of Polarization Sensitive-Optical Coherence Tomography to the Assessment of Phase Retardation in Subpleural Cancer in Rabbits

Park, Jung-Eun; Zhou, Xin; Kwon, Daa Young; Kim, Sung Won; Lee, Haeyoung; Jung, Min-Jung; Tang, Shuo; Ko, Taek Yong; Choi, Jin Hyuk; Kim, Jun Hyeong; Ahn, Yeh‐Chan; Oak, Chulho

doi:10.1007/s13770-020-00318-9

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…The basic principles and achievements of the PSOCT of biological objects over the past 25 years have been consistently set out in a series of review articles [5][6][7]. This laser polarization-interference technique not only makes it possible to obtain layered images from small depths (up to 2 mm) of biological tissues [8,9] but also provides the possibility of obtaining coordinate distributions of the Jones and Mueller matrix elements and anisotropy maps. This was most effectively demonstrated in studies of transparent eye tissues [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Mueller matrix polarization interferometry of optically anisotropic architectonics of biological tissue object fields: the fundamental and applied aspects

Ushenko,

Dubolazov,

Zheng

et al. 2024

Front. Phys.

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The presented results concern the diagnostic application of vector-parameter polarization mapping and polarization-interference phase scanning applied to layered azimuths and ellipticity polarization maps obtained with the help of digital holographic reproduction for phase-inhomogeneous tissue layers in the female reproductive system. The differential diagnosis of the pathological changes in optically anisotropic polycrystalline tissue components, specifically “benign (fibroids) and precancerous (endometriosis),” was investigated. All studies within the representative groups of the histological samples were conducted using circularly polarized laser radiation, ensuring azimuthal invariance and polarization measurement reliability. Integral and layered maps and polarization azimuths and ellipticity random variable distribution histograms were obtained from the microscopic images of histological sections from the female reproductive system tumors. Systematized tables present statistical moments which characterize azimuths and ellipticity polarization maps for fibroids and endometriosis tissues. It is shown that the statistical parameters (diagnostic markers) are the most sensitive to benign and precancerous changes in the female reproductive system tissues. The results of information analysis, including the accuracy determination of the diagnostic vector-parameter polarization and polarization-interference methods for detecting and differentiating the samples of fibroids and precancerous endometriosis tissues in the female reproductive system, are presented.

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Section: Introductionmentioning

confidence: 99%

Mueller matrix polarization interferometry of optically anisotropic architectonics of biological tissue object fields: the fundamental and applied aspects

Ushenko,

Dubolazov,

Zheng

et al. 2024

Front. Phys.

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“…Recently, combination therapies comprising multiple methods, such as photodynamic therapy (PDT) have been tried to be complements to chemotherapy because they can increase the therapeutic efficiency by enabling the intelligent drug delivery to target sites and fewer side effects [ 7 – 9 ]. The PDT currently used in the clinic as an adjunctive therapy has been attractive in the treatment of various cancer because it is possible to expose the photosensitizer to light and activate it in the tumor tissue [ 10 ] although the photosensitizer (PS) should satisfy several points, such as purity, tumor selectivity, minimal toxicity, rapid systemic clearance and activation at a long wavelength with high photochemical reactivity [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro photodynamic therapy of methylene blue-loaded acetyl resistant starch nanoparticles

Park¹,

Babu³

et al. 2022

Biomater Res

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Background Combination therapies comprising multiple methods, such as photodynamic therapy have been applied to be complements chemotherapy as they increase the therapeutic efficiency by enabling the intelligent drug delivery to target sites by exposing the photosensitizer to light and activating it in the tumor tissue. This study evaluated in vitro photodynamic therapy of methylene blue (MB)-loaded acetyl resistant starch (ARS) nanoparticles (NPs). Methods ARS was synthesized by the reaction between resistant starch (RS) and acetic anhydride. MB-loaded ARS NPs and ARS NPs were prepared by a single emulsion method. Synthesized ARS was measured by NMR. Prepared ARS NPs and MB-loaded ARS NPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), X-ray diffraction, UV/Vis, and circular dichroism (CD). MB-loaded ARS NPs were treated in mouse colon cancer cells (CT-26) and they were treated under near-infrared (NIR) laser irradiation. Results Synthesis of ARS was confirmed by NMR and the degree of substitutions in the ARS was 7.1. The morphologies of ARS NPs observed by TEM were spherical shapes and the particle sizes of ARS NPs were 173.4 nm with a surface charge of − 17.24 mV. The d-spacing of ARS NPs was smaller than those of RS and the conformational changes of RS occurred by the formation of self-assembled polymeric NPs with induction of CD of the MB by chiral ARS NPs. The phototoxicity of CT-26 cells treated by MB-loaded ARS NPs dramatically decreased in a dose-dependent manner under NIR laser irradiation compared to free MB. Conclusion This study demonstrated the ordered nanosized structures in the ARS NPs and conformational change from random coil structure of RS to alpha-helices one of ARS occurred and CD of the achiral MB was induced. The MB-loaded ARS NPs showed a higher generation of reactive oxygen species (ROS) in the CT-26 cells than free MB with the NIR laser irradiation and resulting in phototoxicity under irradiation.

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Insights into polycrystalline microstructure of blood films with 3D Mueller matrix imaging approach

Ushenko,

Sdobnov,

Soltys

et al. 2024

Sci Rep

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This study introduces a novel approach in the realm of liquid biopsies, employing a 3D Mueller-matrix (MM) image reconstruction technique to analyze dehydrated blood smear polycrystalline structures. Our research centers on exploiting the unique optical anisotropy properties of blood proteins, which undergo structural alterations at the quaternary and tertiary levels in the early stages of diseases such as cancer. These alterations manifest as distinct patterns in the polycrystalline microstructure of dried blood droplets, offering a minimally invasive yet highly effective method for early disease detection. We utilized a groundbreaking 3D MM mapping technique, integrated with digital holographic reconstruction, to perform a detailed layer-by-layer analysis of partially depolarizing dry blood smears. This method allows us to extract critical optical anisotropy parameters, enabling the differentiation of blood films from healthy individuals and prostate cancer patients. Our technique uniquely combines polarization-holographic and differential MM methodologies to spatially characterize the 3D polycrystalline structures within blood films. A key advancement in our study is the quantitative evaluation of optical anisotropy maps using statistical moments (first to fourth orders) of linear and circular birefringence and dichroism distributions. This analysis provides a comprehensive characterization of the mean, variance, skewness, and kurtosis of these distributions, crucial for identifying significant differences between healthy and cancerous samples. Our findings demonstrate an exceptional accuracy rate of over $$90\%$$ 90 % for the early diagnosis and staging of cancer, surpassing existing screening methods. This high level of precision and the non-invasive nature of our technique mark a significant advancement in the field of liquid biopsies. It holds immense potential for revolutionizing cancer diagnosis, early detection, patient stratification, and monitoring, thereby greatly enhancing patient care and treatment outcomes. In conclusion, our study contributes a pioneering technique to the liquid biopsy domain, aligning with the ongoing quest for non-invasive, reliable, and efficient diagnostic methods. It opens new avenues for cancer diagnosis and monitoring, representing a substantial leap forward in personalized medicine and oncology.

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Application of Polarization Sensitive-Optical Coherence Tomography to the Assessment of Phase Retardation in Subpleural Cancer in Rabbits

Cited by 7 publications

References 13 publications

Mueller matrix polarization interferometry of optically anisotropic architectonics of biological tissue object fields: the fundamental and applied aspects

Mueller matrix polarization interferometry of optically anisotropic architectonics of biological tissue object fields: the fundamental and applied aspects

In vitro photodynamic therapy of methylene blue-loaded acetyl resistant starch nanoparticles

Insights into polycrystalline microstructure of blood films with 3D Mueller matrix imaging approach

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