Application of platinum nanoparticles as affinity probe and matrix for direct analysis of small biomolecules and microwave digested proteins using matrix-assisted laser desorption/ionization mass spectrometry

Abstract

We report the use of platinum nanoparticles (PtNPs) for analysis of amino acids, peptides, proteins and microwave digested proteins (lysozyme and bovine serum albumin) without any tedious washing and separation procedures prior to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). In the present study, PtNPs play three functions, such as matrix, affinity probe and acceleration of protein digestion by absorbing the microwave irradiation. Good signal intensity of the target molecules from the sample was obtained when laser energy, NPs concentration and incubation time were set to 35 μJ, 25 nM and 30 min, respectively. In addition, higher numbers of peptide sequence were obtained for microwave digested lysozyme protein using PtNPs as compared to previously reported methods for analysis of digested protein in MALDI-MS. Thus, the present method is a simple, rapid and one step preparation method for the analysis of amino acids, peptides, proteins and digested proteins in MALDI-TOF-MS without the need for any tedious purifications and washing procedures.

Nanomaterials as Assisted Matrix of Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Analysis of Small Molecules

“…Among various metal-or metal oxide-based nanomaterials (e.g., Au [51,52,53,54,55], Ag [56], Pt [57,58,59], TiO 2 [60,61,62], ZnO [63], CdS [64], and HgTe [65] NPs) that explored as matrices for LDI-TOF MS, TiO 2 and gold NPs (Au NPs) attracted much more attention because their intrinsic advantages, such as large surface area, strong absorption efficiency in UV-visible region, high chemical stability, easy modification and preparation.…”

Nanomaterial-based miniaturized extraction and preconcentration techniques coupled to matrix-assisted laser desorption/ionization mass spectrometry for assaying biomolecules

“…This work concluded that the AgNPs showed high affinity to trap and to concentrate bacterial proteins, resulting in the enhancement of protein signals in the MALDI-MS spectra. Similarly, we also described the utility of PtNPs as affinity probes for analysis of amino acids, peptides, proteins [insulin (5.8 kDa), ubiquitin (8.5 kDa) and lysozyme (13.5 kDa)] and microwave-digested proteins [lysozyme and bovine serum albumin (BSA)] by MALDI-MS [65]. In this method, laser energy (35 µJ), NP concentration (25 nM) and incubation time (30 min) were the governing factors for the production of analyte signals with high intensities.…”

Less common applications of monoliths: V. Monolithic scaffolds modified with nanostructures for chromatographic separations and tissue engineering

“…The target analytes extracted using nanoparticles are released from their surface or directly admixed to low molecular weight organic matrices on the matrix‐assisted laser desorption/ionization (MALDI) plate prior to MS detection. However, nanoparticles may serve not only as affinity probes for selective extraction but also as matrices for direct analysis by surface‐assisted laser desorption/ionization (SALDI) MS []. Chemically modified silicon nanoparticles were used in silicon nanoparticle‐assisted laser desorption/ionization (SPALDI) MS analysis of sulfonic and fatty acids [].…”