Application of platelet-rich plasma (PRP) improves self-renewal of human spermatogonial stem cells in two-dimensional and three-dimensional culture systems

Khadivi, Farnaz; Koruji, Morteza; Akbari, Mohammad; Jabari, Ayob; Talebi, Ali; Movassagh, Sepideh Ashouri; Boroujeni, Amin Panahi; Feizollahi, Narjes; Nikmahzar, Aghbibi; Pourahmadi, Mohammad; Abbasi, Mehdi

doi:10.1016/j.acthis.2020.151627

Cited by 19 publications

(8 citation statements)

References 59 publications

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“…Flow cytometry analysis of the cells performed after the two‐step digestion (day 0) indicated that 17.2% of human testicular cells was positive for PLZF marker, and the results of our study confirmed the results of other studies in this field (Jabari et al, 2020; Khadivi et al, 2020). To eliminate most of the differentiated cells from the isolated cells, which were obtained after enzymatic digestion, the cellular suspension was cultivated for 2 weeks in proliferation basic medium.…”

Section: Discussionsupporting

confidence: 90%

Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds

Bashiri¹,

Zahiri

Allahyari

et al. 2022

Andrologia

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Section: Discussionsupporting

confidence: 90%

Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds

Bashiri¹,

Zahiri

Allahyari

et al. 2022

Andrologia

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“…Furthermore, cell viability increased in the ECM‐PRP group compared to other groups, possibly due to the effects of PRP growth factors. In this line, khadivi et al indicated that SSCs viability increased significantly when the medium was supplemented with 5% PRP compared to control group after 4 weeks of culture 40 …”

Section: Discussionmentioning

confidence: 97%

“…These results can illustrate that supplemented DTM with PRP can support SSCs differentiation. This finding can originate from the important role of testis ECM components together with the PRP factors affecting SSCs viability and differentiation 3,29,36,40,43 . Apoptosis in SSCs can be induced in long‐term cell culture 34 .…”

Section: Discussionmentioning

confidence: 99%

“…In this line, khadivi et al indicated that SSCs viability increased significantly when the medium was supplemented with 5% PRP compared to control group after 4 weeks of culture. 40 We evaluated in vitro SSCs differentiation and apoptosis by assessing the expression level of pre-meiotic, meiotic, post-meiotic, and apoptotic genes after 4 weeks of culture. In the testis, GFRA1 and PLZF as markers of undifferentiated spermatogonia are expressed in As, Apr, and Aal spermatogonia.…”

Section: Discussionmentioning

confidence: 99%

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Differentiation of human spermatogonial stem cells using a human decellularized testicular scaffold supplemented by platelet‐rich plasma

et al. 2023

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Background: Effective culture systems for attachment, migration, proliferation, and differentiation of spermatogonial stem cells (SSCs) can be a promising therapeutic modality for preserving male fertility. Decellularized extracellular matrix (ECM) from native testis tissue creates a local microenvironment for testicular cell culture. Furthermore, platelet-rich plasma (PRP) contains various growth factors for the proliferation and differentiation of SSCs. Methods:In this study, human testicular cells were isolated and cultured for 4 weeks, and SSCs were characterized using immunocytochemistry (ICC) and flow cytometry. Human testicular tissue was decellularized (0.3% SDS, 1% Triton), and the efficiency of the decellularization process was confirmed by histological staining and DNA content analysis. SSCs were cultured on the human decellularized testicular matrix (DTM) for 4 weeks. The viability and the expression of differentiation genes were evaluated by MTT and real-time polymerase chain reaction (PCR), respectively.Results: Histological evaluation and DNA content analysis showed that the components of ECM were preserved during decellularization. Our results showed that after 4 weeks of culture, the expression levels of BAX, BCL-2, PLZF, and SCP3 were unchanged, while the expression of PRM2 significantly increased in the cells cultured on DTM supplemented with PRP (ECM-PRP). In addition, the expression of GFRA1 was significantly decreased in the ECM group compared to the control and PRP groups. Furthermore, the MTT test indicated that viability was significantly enhanced in cells plated on DTM supplemented with PRP. Conclusion:Our study demonstrated that DTM supplemented with PRP can provide an effective culture system for the differentiation and viability of SSCs.

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“…The reason Oct4 is well placed as a leading contributor to ovarian remodeling is that human gonadal (testicular) cells express Oct4 (and c-KIT ) after in vitro culture with PRP ( Khadivi et al 2020 ). It is plausible that a parallel response occurs in adult ovarian tissues following intraovarian activated platelet-derived cytokines ( Sills & Wood 2022 ).…”

Section: Prp – Ovaria In Aeternum ?mentioning

confidence: 99%

Epigenetics, ovarian cell plasticity, and platelet-rich plasma: Mechanistic theories

Sills¹,

Wood

2022

Reproduction and Fertility

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Ovarian platelet-rich plasma (PRP) is claimed to restore fertility potential by improving reserve, an effect perhaps mediated epigenetically by platelet-discharged regulatory elements rather than gonadotropin activated G-protein coupled receptors, as with stimulated in vitro fertilization (IVF). The finding that fresh activated platelet releaseate includes factors able to promote developmental signaling networks necessary to enable cell pluripotency tends to support this theory. The mechanistic uncertainty of intraovarian PRP notwithstanding, at least two other major challenges confront this controversial intervention. First is to clarify how perimenopausal ovarian function is reset to levels consistent with ovulation. Perhaps a less obvious secondary problem is to confine this renewal such that any induced recalibration of cellular plasticity is kept within acceptable physiologic bounds. Thus, any ‘drive’ to ovarian rejuvenation must incorporate both accelerator and brake. Ovarian aging may be best viewed as a safeguard against pathologic overgrowth, where senescence operates as an evolved tumor-suppression response. While most ovary cells reach the close of their metabolic lifespan with low risk for hypertrophy, enhanced lysosomal activity and the pro-inflammatory ‘Senescence Associated Secretory Phenotype’ usually offsets this advantage over time. But is recovery of ovarian fitness possible, even if only briefly prior to IVF? Alterations in gap junctions, bio-conductive features, and modulation of gene regulatory networks after PRP use in other tissues are discussed here alongside early data reported from reproductive medicine.

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Application of platelet-rich plasma (PRP) improves self-renewal of human spermatogonial stem cells in two-dimensional and three-dimensional culture systems

Cited by 19 publications

References 59 publications

Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds

Proliferation of human spermatogonial stem cells on optimized PCL/Gelatin nanofibrous scaffolds

Differentiation of human spermatogonial stem cells using a human decellularized testicular scaffold supplemented by platelet‐rich plasma

Epigenetics, ovarian cell plasticity, and platelet-rich plasma: Mechanistic theories

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