Application of Photosynthetic Pigment Analysis Using a HPLC and CHEMTAX Program to Studies of Phytoplankton Community Composition

Lee, Yong-Woo; Park, Mi-Ok; Kim, Yoon-Suk; Kim, Seong-Su; Kang, Chang‐Keun

doi:10.7850/jkso.2011.16.3.117

Cited by 21 publications

(18 citation statements)

References 26 publications

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“…CHEMTAX analysis was performed using the averaged pigment concentrations from each TP. Analysis was performed 1022 N. Schuback et al: Diurnal variation in the coupling of photosynthetic electron transport essentially as described in Taylor et al (2013).The initial pigment ratio matrix, specific to North Pacific phytoplankton isolates, was taken from Table 5 in Lee et al (2011).…”

Section: [Chl A] and High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

et al. 2016

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Abstract. Active chlorophyll a fluorescence approaches, including fast repetition rate fluorometry (FRRF), have the potential to provide estimates of phytoplankton primary productivity at an unprecedented spatial and temporal resolution. FRRF-derived productivity rates are based on estimates of charge separation in reaction center II (ETR RCII ), which must be converted into ecologically relevant units of carbon fixation. Understanding sources of variability in the coupling of ETR RCII and carbon fixation provides physiological insight into phytoplankton photosynthesis and is critical for the application of FRRF as a primary productivity measurement tool. In the present study, we simultaneously measured phytoplankton carbon fixation and ETR RCII in the iron-limited NE subarctic Pacific over the course of a diurnal cycle. We show that rates of ETR RCII are closely tied to the diurnal cycle in light availability, whereas rates of carbon fixation appear to be influenced by endogenous changes in metabolic energy allocation under iron-limited conditions. Unsynchronized diurnal oscillations of the two rates led to 3.5-fold changes in the conversion factor between ETR RCII and carbon fixation (K c /n PSII ). Consequently, diurnal variability in phytoplankton carbon fixation cannot be adequately captured with FRRF approaches if a constant conversion factor is applied. Utilizing several auxiliary photophysiological measurements, we observed that a high conversion factor is associated with conditions of excess light and correlates with the increased expression of non-photochemical quenching (NPQ) in the pigment antenna, as derived from FRRF measurements. The observed correlation between NPQ and K c /n PSII requires further validation but has the potential to improve estimates of phytoplankton carbon fixation rates from FRRF measurements alone.

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Section: [Chl A] and High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

et al. 2016

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“…Based on pigment composition and concentrations, phytoplankton community composition was estimated from the ratio of each biomarker pigment to chlorophyll a, using CHEMTAX software [Mackey et al, 1996; Journal of Geophysical Research: Oceans [Lee et al, 2011] were used as the input pigment ratios of the CHEMTAX program. Cluster analysis of phytoplankton assemblage was applied to each station and depth, and any dissimilarity between stations and depths was calculated using a Bray-Curtis similarity matrix.…”

Section: Pigment and Chemotaxonomic Analysis On Size-fractionated Sammentioning

confidence: 99%

“…Twelve diagnostic biomarker pigments-chlorophyll a, fucoxanthin, 19 0hexanoyloxy-fucoxanthin, 19 0 -butanoyloxy-fucoxanthin, neoxanthin, peridinin, chlorophyll b, prasinoxanthin, lutein, violaxanthin, alloxanthin, and zeaxanthin-were used. Class-specific pigment ratios determined from various species collected around the Korean peninsula [Lee et al, 2011] were used as the input pigment ratios of the CHEMTAX program. Cluster analysis of phytoplankton assemblage was applied to each station and depth, and any dissimilarity between stations and depths was calculated using a Bray-Curtis similarity matrix.…”

Section: Pigment and Chemotaxonomic Analysis On Size-fractionated Sammentioning

confidence: 99%

Summer primary productivity and phytoplankton community composition driven by different hydrographic structures in the East/Japan Sea and the Western Subarctic Pacific

et al. 2014

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The East/Japan Sea (EJS) is a highly productive marginal sea in the northwest Pacific, consisting of three basins (Ulleung Basin: UB, Yamato Basin: YB, and Japan Basin: JB). To find causes of the reportedly high primary productivity in summer in the EJS, especially in the UB, we measured primary productivity, phytoplankton composition, and other environmental variables. The water column was strongly stratified in the EJS compared with the Western Subarctic Pacific (WSP). Integrated primary productivity was two times higher in the EJS (612 mg C m 22 d 21 ) than in the WSP (291 mg C m 22 d 21 ). The vertical distributions of physicochemical and biological factors confirmed that production in the subsurface chlorophyll maximum layer in the study regions was an important factor regulating primary productivity within the water column. While picoplankton (<2.7 mm) dominated in the WSP, JB, and YB, micro/nanoplankton (2.7 mm) dominated in the UB. Contribution by picoplankton to total biomass and primary productivity in the UB was significantly lower than in the other regions. CHEMTAX analysis using marker pigments showed that diverse phytoplankton groups inhabited the study regions. Cluster and canonical correspondence analyses showed high correlation between the spatial variation in phytoplankton assemblages with the water mass properties mainly represented by water temperature and nitrate concentration. Overall, our results suggest that the hydrographic structure of water column in the study region is an important controlling factor of the biomass and productivity of phytoplankton as well as their diversity in size and taxonomic groups.

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“…chlorophyll a, fucoxanthin, 19 -hexanoyloxy-fucoxanthin, 19 -butanoyloxy-fucoxanthin, neoxanthin, peridinin, chlorophyll b, prasinoxanthin, lutein, violaxanthin, alloxanthin, and zeaxanthin) were used for the programme. The class-specific pigment ratios, modified from the values in Lee et al (2011) were used as the input pigment ratios of the CHEMTAX programme.…”

Section: Chemotaxonomic Analysis Of Phytoplankton Pigmentsmentioning

confidence: 99%

Monthly measured primary and new productivities in the Ulleung Basin as a biological "hot spot" in the East/Japan Sea

Kwak

Lee²,

Park

et al. 2013

Biogeosciences

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Abstract. The Ulleung Basin (UB), located in the southwestern part of the East/Japan Sea (EJS), is considered having an unusually high productivity for a deep basin. Recently changes have been reported in physical, chemical, and biological properties. Here we measured the primary and new productivities in the UB using a 13C-15N dual isotope tracer technique. Measurements took place every month for the first time throughout a year for a better estimate of the annual primary production in the EJS. Temporal variations of temperature, salinity, and density (σt) in the study area were highly seasonal as expected for an ocean in the temperate zone. Nutrient distributions reflected these seasonal fluctuations in the vertical structure of the water column. Diatoms were in general the most dominant phytoplankton ranging from 15.5 to 82.2% with an average of 42.0% (S.D. = ±9.9%). Based on those average daily productivities from our monthly measurements, the annual primary, new, and regenerated production in the UB were 273.0 g C m−2 yr−1, 62.6 g N m−2 yr−1, and 48.7 g N m−2 yr−1, respectively. Our estimated high f ratio (0.59) in the UB, indicated that the predominant nitrogen source for primary production was nitrate. This is comparable with the nitrogen source in a productive coastal-upwelling region. New carbon production by phytoplankton is estimated as 145.6 g C m−2 yr−1 (S.D. = ±40.8 g C m−2 yr−1) which indicates that a large portion (53.9%) of the total annual primary production might potentially be exported from the diatom-dominated euphotic zone to a deeper zone in the UB. Further intense integrated field observations will be necessary to improve our understanding of the current marine ecosystem in the UB as an important biological production area in the EJS.

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Application of Photosynthetic Pigment Analysis Using a HPLC and CHEMTAX Program to Studies of Phytoplankton Community Composition

Cited by 21 publications

References 26 publications

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

Summer primary productivity and phytoplankton community composition driven by different hydrographic structures in the East/Japan Sea and the Western Subarctic Pacific

Monthly measured primary and new productivities in the Ulleung Basin as a biological "hot spot" in the East/Japan Sea

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Application of Photosynthetic Pigment Analysis Using a HPLC and CHEMTAX Program to Studies of Phytoplankton Community Composition

Cited by 21 publications

References 26 publications

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

Diurnal variation in the coupling of photosynthetic electron transport and carbon fixation in iron-limited phytoplankton in the NE subarctic Pacific

Summer primary productivity and phytoplankton community composition driven by different hydrographic structures in the East/Japan Sea and the Western Subarctic Pacific

Monthly measured primary and new productivities in the Ulleung Basin as a biological &quot;hot spot&quot; in the East/Japan Sea

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Monthly measured primary and new productivities in the Ulleung Basin as a biological "hot spot" in the East/Japan Sea