In recent years, surfactant-based sensors have played a vital role in every field of research. Surfactants are chemical compounds that act as an interface between the two states of matter to reduce surface tension. Surfactants are usually organic compounds that are amphiphilic in nature. To be precise, surfactant-based sensors have a wide range of applications in chemical and biochemical detection. We can assess the effectiveness and efficiency of surfactants using tensiometers, which measure surface tension. The construction and optimization of emulsions by precisely measuring the interfacial tension are carried out using various characterization methods. Biologically, pulmonary surfactants are produced by humans in the lungs to promote lung capacity. Here, our aim is to achieve a standard platform to fabricate cost-effective, high sensitivity, high stability, enhanced lifespan, reusable sensing devices with quick response characteristics. Simple, stable, cost-effective, and biodegradable properties have become prerequisites for the fabrication of effective surfactant-based sensors. In the present chapter, surfactant-based sensors and their properties with regard to classification, characterization, stability, lifetime, reuse, and their importance in the detection of chemicals and biochemicals are discussed.