Application of peptide nucleic acids containing azobenzene self-assembled electrochemical biosensors in detecting DNA sequences

Abstract

on the 3-mercaptopropionic acid self-assembled monolayer modified gold electrode with the helps of N-(3-dimethylaminopropy1)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS), and the hybrid was coded as N-PNAs/DNA. Using [Fe(CN) 6 ] 4−/3− (1:1 ) as the electrochemical indicator, the electrochemical properties of the N-PNAs self-assembled monolayer (N-PNAs-SAMs) and N-PNAs/DNA hybridization system under the conditions of before and after UV light irradiation were characterized with cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectra (EIS). Resultsshowed that the redox currents decreased with the increase of irradiation time, suggesting that the ability of the charge transfer on the electrode surface was weakened and the conformation of hybrid system had been changed, and the control of PNAs/DNA hybridization could be realized by UV light irradiation.azobenzene monomer, peptide nucleic acid, hybridization, gold electrode, electrochemical biosensors

Density functional theoretical studies on photoswitching and charge migration dynamics of thio and selenoureidopeptides

“…The introduction of photoswitchable elements in peptides are interesting, which gives control over their structure and bioactivities such as catalysis, protein folding, receptor‐ligand interaction, and ion‐channel gating . The switchable nature has a profound effect in increasing their prosperity in advanced technologies, which include reversible biosensors, photoactivated medicines, and molecular devices . To date, many biological photoswitches based on azobenzene, spiropyrene‐centred peptidomimetics have been explored both in vivo and in vitro .…”

Improved electrochemical performances of polyaniline nanotubes-poly-L-lysine composite for label-free impedance detection of DNA hybridization

“…Considerable interest has been focused on analytical methods for rapid, simple, and low-cost transgenic plant detection, especially in food and the environment [1]. Among various detection methods, DNA electrochemical techniques possess particular advantages such as low cost, small size, and convenience for integration and microminiaturization [2][3][4]. Hence, DNA electrochemical biosensors have become one of the most significant detection methods for transgenic plant products.…”