Application of PCR for various neurotropic viruses on the diagnosis of viral meningitis

Honzumi, Ken; Sato, Masatoki; Katayose, Masahiko; Kato, Koichi; Suzuki, Hitoshi

doi:10.1016/s1386-6532(98)00048-8

Cited by 42 publications

(27 citation statements)

References 13 publications

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“…IgM capture ELISA performed on sera and CSFs has been shown to be useful in early diagnosis of neurological infections [Heyman, 1997;Jones et al, 1999], but sometimes the availability of such tests is not possible for all of the viruses (e.g., enteroviruses). Therefore, the use of PCR for the detection of viral DNA or RNA is a quick and sensitive molecular technique that can be very useful for the diagnosis of viral meningitis [Hosoya et al, 1998;Read and Kurtz, 1999]. However, it is necessary to improve the clinical usefulness of the PCR due to the lack of a comparable gold-standard test [Jeffery et al, 1997].…”

Section: Discussionmentioning

confidence: 99%

“…Standard diagnostic procedures, such as viral culture or serology, are often insufficient for the circumstantial or retrospective identification of a viral infection of the central nervous system [Chonmaitree et al, 1989]. Molecular techniques appear to be valid support useful for the accurate detection of the viral agent in the CSF, considering the rapidity of execution and their sensitivity [Hosoya et al, 1998;Read and Kurtz, 1999]. We applied the molecular techniques of polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) for the detection of viruses as etiologic agents in 277 cases of meningitis which occurred in Siena (Italy) during the period from 1995 to 1998 in order to evaluate the viral agent that is the most frequent cause of meningitis in this geographic area.…”

Section: Introductionmentioning

confidence: 99%

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Detection of neurotropic viruses circulating in Tuscany: The incisive role of Toscana virus

et al. 2000

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Acute meningitis is perhaps the most frequent among central nervous system infections. We report a study considering 277 cases of meningitis hospitalized in the southern Tuscany area (Italy) during the period from 1995 to 1998 investigated by tissue culture and polymerase chain reaction (PCR) methods. The cytochemical analysis of the cerebrospinal fluid samples suggested the diagnosis of aseptic meningitis, recognized as viral meningitis in 104 cases by detection of viral DNA or RNA. The results collected by tissue culture technique, available for 95 clinical samples, reported a positive isolation for only 12 cases. The viruses identified in the neurological infection were Toscana virus (81%), enterovirus (12%), mumps virus (3%), measles virus (1%), and herpes virus type 1 (3%). These data demonstrate the incisive role of the RNA viruses as the cause of meningitis, and overall the relevance of Toscana virus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of neurotropic viruses circulating in Tuscany: The incisive role of Toscana virus

et al. 2000

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“…The sensitivity of PCR-Fukushima, calculated from extractions of serial dilutions of titrated echovirus type 7, corresponded to 10 Ϫ3 tissue culture infective dose. 4 To eliminate and to detect laboratory contamination leading to false-positive PCR results, negative controls were included for each step of the assay.…”

Section: Pcr For Detection Of the Enteroviral Genomementioning

confidence: 99%

Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

et al. 2001

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ABSTRACT. Objective. To clarify the relationship between enteroviral infection and febrile seizures.Study Design. Cerebrospinal fluid (CSF), serum, throat swab, and rectal swab samples were collected for virologic examination from 67 children with febrile seizures from April 1997 to March 1999. Those samples were examined for the presence of enterovirus using cell culture and 2 polymerase chain reaction (PCR) methods.Results. No enterovirus was isolated from cell culture of CSF, throat swab, or rectal swab samples. All samples were screened for the presence of enteroviral sequences using a sensitive PCR method (PCR-Fukushima). We obtained positive results from 14 of 67 CSF samples, 10 of 62 serum samples, 12 of 64 throat swab samples, and 13 of 64 rectal swab samples. Of 21 patients in whom febrile seizures had developed during the summer months (June through August), 13 (61.9%) had positive PCR results in the CSF. Forty-seven of the 49 samples with a positive result using PCR-Fukushima were reexamined independently for the presence of the enteroviral genome using another PCR method (PCR-Mitsubishi). PCR-Mitsubishi had slightly lower sensitivity than PCR-Fukushima but identified genotypes of enterovirus by subsequent sequence analysis of the PCR products. The presence of the enteroviral genome was confirmed in 39 of the samples (83.0%). In 8 of the 9 enteroviruses detected in the CSF and/or serum samples using PCR-Mitsubishi, the genotypes were identified as coxsackieviruses group A, which are usually difficult to isolate using cell culture methods.Conclusions. These findings proved that the causative agents of febrile illness associated with seizures in summer were primarily enteroviruses, especially coxsackieviruses group A, and that febrile seizures might be caused by enteroviral infection in the central nervous system. Pediatrics 2001;107(1). URL: http://www. pediatrics.org/cgi/content/full/107/1/e12; enterovirus, coxsackievirus group A, febrile seizures, polymerase chain reaction.ABBREVIATIONS. CNS, central nervous system; CSF, cerebrospinal fluid; PCR, polymerase chain reaction; bp, base pair. E nteroviruses-polioviruses, echoviruses, coxsackieviruses group A and group B, and enteroviruses-are responsible for significant and frequent human illness, including various neurologic manifestations. The most common neurologic illness is aseptic meningitis. Encephalitis and other manifestations, such as paralysis, Guillain-Barré syndrome, transverse myelitis, cerebellar ataxia, and peripheral neuritis, also occur. 1 The association of enteroviral infection in the central nervous system (CNS) with febrile seizures, however, has not been demonstrated. In general, the causative agent of the infection is not detected in the CNS of patients with febrile seizures using standard techniques.We previously reported that the enteroviral genome was detected in cerebrospinal fluid (CSF) samples collected from some children with transient neurologic complications, such as seizures and altered states of consciousness associated ...

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“…Measles, rubella and mumps were also serologically excluded. However, RT-PCR [2] revealed enterovirus RNA in 2 CSF samples obtained in August and November 1995. Although enterovirus RNA was not detected after 1998, 3 CSF samples exhibited positivity for autoantibodies against GluR IgG-2 from 1998 to 1999 [3] .…”

Section: Enteroviral Chronic Limbic Encephalitismentioning

confidence: 98%

A Chronic Progressive Case of Enteroviral Limbic Encephalitis Associated with Autoantibody to Glutamate Receptor ε2

et al. 2007

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Application of PCR for various neurotropic viruses on the diagnosis of viral meningitis

Cited by 42 publications

References 13 publications

Detection of neurotropic viruses circulating in Tuscany: The incisive role of Toscana virus

Detection of neurotropic viruses circulating in Tuscany: The incisive role of Toscana virus

Association of Nonpolio Enteroviral Infection in the Central Nervous System of Children With Febrile Seizures

A Chronic Progressive Case of Enteroviral Limbic Encephalitis Associated with Autoantibody to Glutamate Receptor ε2

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