Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest

Nuthan, Bettadapura Rameshgowda; Rakshith, Devaraju; Marulasiddaswamy, Kuppuru Mallikarjunaiah; Rao, H.C. Yashavantha; Ramesha, K.P.; Mohana, N. Chandra; Siddappa, Shiva; Darshan, Doreraj; Kumara, Kigga Kaadappa Sampath; Satish, S.

doi:10.1093/chromsci/bmaa045

Cited by 18 publications

(17 citation statements)

References 29 publications

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“…Several studies have been conducted on the effect of particle volume fraction on the mechanical properties of bamboo particle composite materials, both pure bamboo particles and a combination of 2 different types of particles [13,[19][20][21][22], types of thermosetting and thermoplastic matrices [18,[22][23][24], fiber shape and size [25][26][27], and fiber orientation [28][29][30], all of which target an increase in mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

Polymer Composite Fabrication Reinforced with Bamboo Fiber for Particle Board Product Raw Material Application

2021

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Bamboo particles as reinforcement in composite materials are prospective to be applied to particleboard products in the industry. This study aimed to synthesize bamboo particle reinforced polymer composites as a substitute for particleboard products, which still use wood as a raw material. The parameters of the composite synthesis process were varied with powder sizes of 50, 100, and 250 mesh, each mesh with volume fractions of 10, 20, and 30%, matrix types of polyester and polypropylene, Tali Bamboo, and Haur Hejo Bamboo as reinforcements. Characterization included tensile strength, flexural strength, and morphology. Particleboard products were tested based on JIS A 5908-2003, including density testing, moisture content, thickness expansion after immersion in water, flexural strength in dry and wet conditions, bending Young’s modulus, and wood screw holding power. The results showed that the maximum flexural and tensile strength values of 91.03 MPa and 30.85 MPa, respectively, were found in polymer composites reinforced with Tali bamboo with the particle size of 250 mesh and volume fraction 30%. Particleboard made of polypropylene and polyester reinforced Tali Bamboo with a particle size of 250 mesh and a volume fraction of 30% composites meets the JIS A 5908-2003 standard.

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Section: Introductionmentioning

confidence: 99%

Polymer Composite Fabrication Reinforced with Bamboo Fiber for Particle Board Product Raw Material Application

2021

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“…Coriloxin ( Figure 1 A), which is derived from the mycoendophytic Xylaria sp. NBRTSB-20, is an antimicrobial metabolite [ 9 ]. However, evidence of its antitumor effects on lung cancer cells is scant.…”

Section: Resultsmentioning

confidence: 99%

“…A study observed that ethyl acetate extract from the fermented broth of T. effusa YMJ1179 inhibited the growth of A549 lung cancer cells [ 7 ]. Coriloxin, an antimicrobial metabolite, has been isolated from this fermented broth [ 8 , 9 ]. Furthermore, coriloxin has been isolated from various xylariaceous, endophytic fungi, including strains YUA-026, Xylaria sp.…”

Section: Introductionmentioning

confidence: 99%

Coriloxin Exerts Antitumor Effects in Human Lung Adenocarcinoma Cells

Kuo

Wang

Peng

et al. 2022

IJMS

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Both in Taiwan and around the world, lung cancer is a primary cause of cancer-related deaths. In Taiwan, the most prevalent form of lung cancer is lung adenocarcinoma, a type of non-small-cell lung carcinoma. Although numerous lung cancer therapies are available, their clinical outcomes are unsatisfactory. Natural products, including fungal metabolites, are excellent sources of pharmaceutical compounds used in cancer treatment. We employed in vitro cell invasion, cell proliferation, cell migration, cell viability, and colony formation assays with the aim of evaluating the effects of coriloxin, isolated from fermented broths of Nectria balsamea YMJ94052402, on human lung adenocarcinoma CL1-5 and/or A549 cells. The potential targets regulated by coriloxin were examined through Western blot analysis. The cytotoxic effect of coriloxin was more efficiently exerted on lung adenocarcinoma cells than on bronchial epithelial cells. Moreover, low-concentration coriloxin significantly suppressed adenocarcinoma cells’ proliferative, migratory, and clonogenic abilities. These inhibitory effects were achieved through ERK/AKT inactivation, epithelial–mesenchymal transition regulation, and HLJ1 expression. Our findings suggest that coriloxin can be used as a multitarget anticancer agent. Further investigations of the application of coriloxin as an adjuvant therapy in lung cancer treatment are warranted.

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“…Also known as immersion bioautography, agar overlay bioautography is a hybrid of both previous methods [ 27 ]. In order to allow a good diffusion of the tested compounds into the agar medium, the plates can be placed in a low temperature for a few hours before incubation.…”

Section: Classificationmentioning

confidence: 99%

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

et al. 2021

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Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

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Application of Optimized and Validated Agar Overlay TLC–Bioautography Assay for Detecting the Antimicrobial Metabolites of Pharmaceutical Interest

Cited by 18 publications

References 29 publications

Polymer Composite Fabrication Reinforced with Bamboo Fiber for Particle Board Product Raw Material Application

Polymer Composite Fabrication Reinforced with Bamboo Fiber for Particle Board Product Raw Material Application

Coriloxin Exerts Antitumor Effects in Human Lung Adenocarcinoma Cells

An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

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