Application of on-line capillary GC-FTIR spectroscopy to lipid analysis

Mossoba, Magdi M.; McDonald, Roderick P.; Yurawecz, M. P.; Kramer, J. K. G.

doi:10.1002/1438-9312(200112)103:12<826::aid-ejlt826>3.0.co;2-p

Cited by 10 publications

(5 citation statements)

References 15 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The FFA were methylated in 10% BF 3 / methanol (15). Identifications in sample test portions were confirmed by GC-FTIR as described previously (16). Derivatives of DMOX were obtained as described previously (3,17).…”

Section: Methodsmentioning

confidence: 99%

“…In conclusion, the identities of the synthesized CLA isomers were confirmed by various combinations of UV spectroscopy, routes of synthesis, GC-EI-MS of DMOX derivatives (3), GC-FTIR of FAME (16), partial hydrogenation, and I 2 isomerization (12). These data were generated to aid in the identification of CLA isomers in foods, dietary supplements, and biological samples, and they were used successfully to test a MeCN chemical-ionization GC-MS-MS technique that produced 32 unique spectra for these 32 synthesized CLA FAME (18).…”

Section: Figmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis, isolation, and GC analysis of all the 6,8‐to 13,15‐cis/trans conjugated linoleic acid isomers

Delmonte

Roach

Mossoba

et al. 2004

Lipids

View full text Add to dashboard Cite

Octadecadienoic acids with conjugated double bonds are often referred to as conjugated linoleic acid, or CLA. CLA is of considerable interest because of potentially beneficial effects reported from animal studies. Analysis of CLA is usually carried out by GC elution of FAME. If the presence of low-level isomers is of interest, a complementary technique such as silverion HPLC is also used. These analyses have been hindered by a lack of well-characterized commercially available reference materials. Described here are the synthesis and isolation of selected 6,8- through 13,15-positional CLA isomers, followed by isomerization of these CLA isomers with iodine to produce all the possible cis,cis, cis,trans, trans,cis, and trans,trans combinations. Also present are the GC retention times of the CLA FAME relative to gamma-linolenic acid (6c,9c,12c-octadecatrienoic acid) FAME using a 100-m CP Sil-88 capillary column (Varian Inc., Lake Forest, CA). These data include all the CLA isomers that have been identified thus far in foods and dietary supplements and should greatly aid in the future analysis of CLA in these products.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

Synthesis, isolation, and GC analysis of all the 6,8‐to 13,15‐cis/trans conjugated linoleic acid isomers

Delmonte

Roach

Mossoba

et al. 2004

Lipids

View full text Add to dashboard Cite

show abstract

“…A common quantitative chemometrics tool used for data analysis is partial least-squares (PLS) regression (Table ). , PLS has been applied to the monitoring and controlling of industrial processes, because the determination of total SFA, trans FA, MUFA, and PUFA is rapid (<5 min), fully automated, and unbiased by the operator. The interface of IR spectrophotometer with a GC allows the characterization of fatty acid double bond geometries directly after chromatography. − The advantage of this technique is its ability to provide IR spectra for each separated FAME …”

Section: Analytical Recognition Of Trans Lipidsmentioning

confidence: 99%

Lipid Geometrical Isomerism: From Chemistry to Biology and Diagnostics

et al. 2013

View full text Add to dashboard Cite

“…The concentration of fat in the colostrum and milk was measured using an ultrasonic milk analyzer (Milkyway‐CP2, Beijing, China). FA composition was determined using gas chromatography, as described earlier (Mossoba et al., 2001 ). The procedure involves lipid extraction, FA methylation, and gas chromatography.…”

Section: Methodsmentioning

confidence: 99%

“…FA composition was determined using gas chromatography, as described earlier (Mossoba et al, 2001). The procedure involves lipid extraction, FA methylation, and gas chromatography.…”

Section: Biochemical Analysesmentioning

confidence: 99%