Application of -omic technologies in postharvest pathology: recent advances and perspectives

Chen, Yong; Zhang, Zhanquan; Tian, Shiping; Li, Boqiang

doi:10.1016/j.cofs.2022.100820

Cited by 7 publications

(2 citation statements)

References 48 publications

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“…Additionally, the occurrence of blue mold decay is frequently associated with contamination of the harvested or processed product with the mycotoxin patulin that causes serious health problems ( 26 , 27 ). P. expansum is a necrotrophic fungal pathogen that infects wounds and secretes a range of virulence factors to kill host plant cells and acquire the nutrients released from the dead cells ( 28 , 29 ). Virulence factors produced by P. expansum are strictly regulated during the infection process by a complex regulatory network involving multiple regulatory factors and control elements, which in turn respond to host signals ( 28 ).…”

Section: Introductionmentioning

confidence: 99%

PeAP1-mediated oxidative stress response plays an important role in the growth and pathogenicity of Penicillium expansum

Chen,

Zhang,

et al. 2023

Microbiol Spectr

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Penicillium expansum is the causal agent of post-harvest blue mold in various fruits and serves as a model for understanding fungal pathogenicity and mycotoxin production. The relevance of oxidative stress response in the growth and virulence of P. expansum has been largely unexplored. Here, we identify the transcriptional factor PeAP1 as a regulator of oxidative stress response in P. expansum . Gene expression and protein abundance of PeAP1, as well as its nuclear localization, are specifically induced by H 2 O 2 . Deletion of PeAP1 results in increased sensitivity to H 2 O 2 , and PeAP1 mutants exhibit a variety of defects in hyphal growth and virulence. PeAP1 prevents the accumulation of both intracellular H 2 O 2 during vegetative growth and host-derived H 2 O 2 during biotrophic growth. Application of an antioxidant glutathione and a NADPH oxidase inhibitor, diphenylene iodonium, to the PeAP1 mutant partially restored fungal growth and virulence. RNA sequencing analysis revealed 144 H 2 O 2 -induced PeAP1 target genes, including four antioxidant-related genes, PeGST1 , PePrx1 , PePrx2 , and PeTRX2 , that were also demonstrated to be involved in oxidative stress response and/or virulence. Collectively, our results demonstrate the global regulatory role of PeAP1 in response to oxidative stress and provide insights into the critical role of the PeAP1-mediated oxidative stress response to regulate growth and virulence of P. expansum . IMPORTANCE Reactive oxygen species are the core of host plant defense and also play a vital role in the successful invasion of host plants by pathogenic fungi. Despite its importance, the relevance of oxidative stress response in fungal growth and virulence is poorly understood in P. expansum . In this study, we reveal that the transcription factor PeAP1 acts as a central regulator of oxidative stress response in P. expansum and that there is a major link between PeAP1-mediated oxidative stress response and fungal growth and virulence. To explore the underlying mechanisms, we performed comparative transcriptomic studies and identified a number of H 2 O 2 -induced PeAP1 target genes, including four novel ones, PePrx1 , PePrx2 , PeGST1 , and PeTRX2 , whose functions were linked to PeAP1 and pathogenicity. These findings provide novel insights into the regulation mechanism of PeAP1 on growth and virulence, which might offer promising targets for control of blue mold and patulin contamination.

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Section: Introductionmentioning

confidence: 99%

PeAP1-mediated oxidative stress response plays an important role in the growth and pathogenicity of Penicillium expansum

Chen,

Zhang,

et al. 2023

Microbiol Spectr

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“…In P. expansum , PePacC was required for virulence, patulin production, conidiation, and vegetative growth [ 9 ]. PacC activates multiple alkaline-expressed genes and represses acid-expressed genes involved in transport, secondary metabolism, and cell wall degradation under neutral to alkaline conditions in P. expansum [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Ena Proteins Respond to PacC-Mediated pH Signaling Pathway and Play a Crucial Role in Patulin Biosynthesis

Zhuo,

Chen,

Xing

et al. 2023

JoF

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Penicillium expansum is a main producer of patulin that causes severe postharvest decay and food safety issues in the fruit industry. Development, pathogenicity, and patulin production of P. expansum are strongly influenced by the PacC-pH signaling pathway. Global transcription factor PacC regulates various fungal biological processes through a complicated molecular network. In the present study, three Ena family genes (PeEnas), PeEnaA, PeEnaB, and PeEnaC, as important downstream targets of PePacC, were identified in P. expansum. Deletion of PeEnaA, PeEnaB, and PeEnaC showed little effect on mycelial growth under alkaline or high salinity conditions, but double and triple deletion of these genes impaired the virulence of P. expansum on apple fruit. Notably, patulin biosynthesis of P. expansum was distinctly inhibited in the deletion mutants of PeEnas. PeEnas regulated expressions of the patulin gene cluster, AP1, CreA, Sge1, and Hog1 at the transcriptional level and played roles in maintaining membrane potential. Overexpression of PeEnaC in ΔPePacC restored the patulin production defect of ΔPePacC. Our results indicated that, as downstream targets of PePacC, the PeEna family proteins play a crucial role in patulin biosynthesis in P. expansum.

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Volatile organic compounds of Bacillus spp. as an emerging antifungal resource play a significant role in fruit postharvest disease control

Ling,

Li,

Jiang

et al. 2023

Food Bioscience

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Application of -omic technologies in postharvest pathology: recent advances and perspectives

Cited by 7 publications

References 48 publications

PeAP1-mediated oxidative stress response plays an important role in the growth and pathogenicity of Penicillium expansum

PeAP1-mediated oxidative stress response plays an important role in the growth and pathogenicity of Penicillium expansum

Ena Proteins Respond to PacC-Mediated pH Signaling Pathway and Play a Crucial Role in Patulin Biosynthesis

Volatile organic compounds of Bacillus spp. as an emerging antifungal resource play a significant role in fruit postharvest disease control

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