Application of nutrigenomics tools in animal
feeding and nutritional research

Zduńczyk, Zenon; Pareek, Chandra Shekhar

doi:10.22358/jafs/66361/2009

Cited by 18 publications

(8 citation statements)

References 43 publications

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“…Animal nutrition research has demonstrated that gene expression can be modified by dietary components (Zduńczyk & Pareek ), but the molecular mechanisms involved are still poorly understood and little is known about the role of plant feed ingredients in the expression of genes involved in modulation of the fish immune system (Montero et al . ).…”

Section: Discussionmentioning

confidence: 99%

Growth performance and expression of immune-regulatory genes in rainbow trout (Oncorhynchus mykiss) juveniles fed extruded diets with varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus)

et al. 2012

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Varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus) meals were evaluated as partial replacements for fishmeal in extruded diets for rainbow trout, with particular emphasis on the effect on growth performance and the expression of three genes associated with immune response. A series of 10 isonitrogenous (450 g kg À1 crude protein) and isolipidic (17 g kg À1 crude lipid) diets were formulated to contain different levels of lupin (150 g kg À1 , 250 g kg À1 and 350 g kg À1 ), rapeseed cake (100 g kg À1 , 200 g kg À1 and 300 g kg À1 ) and pea (50 g kg À1 , 150 g kg À1 and 250 g kg À1 ) meals. The control diet was prepared with fish meal as the sole source of protein. Triplicate groups of fish (37.08 ± 3.58 g) were assigned to each experimental diet. The feeding experiment was conducted for 9 weeks at 14.3 ± 0.4°C. The fish were hand fed three times per day, 6 days per week to apparent satiation level. Growth performance, feed utilization and immunological response were significantly affected by the type of plant protein as well as level of inclusion. Hepatosomatic index (HSI) increased in all groups of fish fed diets with pea and rapeseed cake meal. Dietary inclusion of lupin did not affect the expression of Mx-1 gene. Our results suggest that fish meal can be replaced by lupin in the diet of rainbow trout without any apparent adverse effects on key innate immunological genes.

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Section: Discussionmentioning

confidence: 99%

Growth performance and expression of immune-regulatory genes in rainbow trout (Oncorhynchus mykiss) juveniles fed extruded diets with varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus)

et al. 2012

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“…Nutrigenomic tools (i.e. transcriptomics) are increasingly used to investigate molecular events taking place in a genome receiving nutritional signals and responding to them through characteristic metabolic processes in the organism (12) . Nutrigenomics studies in farmed fish have addressed the replacement of different percentages of FM and/or FO with plant ingredients in diets (13)(14)(15) , and the effects of such replacement are well characterised in the hepatic transcriptome of salmonids (10,(16)(17)(18) and marine species such as sea bass (14) .…”

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confidence: 99%

Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins

et al. 2016

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The whole-body transcriptome of trout alevins was characterised to investigate the effects of long-term feeding of rainbow trout broodstock females a diet free of fishmeal and fish oil on the metabolic capacities of progeny. Effects were studied before first feeding and after 3 weeks of feeding diets containing different proportions of marine and plant ingredients. Feeding alevins plant-based diets resulted in lower fish body weight, irrespective of maternal nutritional history. No differences in whole-body lipids were found between treatments, and the tissue fatty acid profile strongly reflected that of the respective broodstock or first-feeding diets. We showed that the maternal diet history did not significantly affect expressions of any genes before the first feeding. Interestingly, we found an effect of maternal nutritional history on gene expression in alevins after 3 weeks of feeding. The major differences in the transcriptome of alevins from plant-based diet-fed females compared with those from commercial-fed females were as follows: (i) down-regulation of genes involved in muscle growth/contraction and (ii) up-regulation of genes involved in carbohydrate and energy metabolism related to the delay in growth/development observed with plantbased diets. Our findings also showed an effect of the first-feeding diets, irrespective of maternal nutritional history. Specifically, the introduction of plant ingredients resulted in the up-regulation of genes involved in amino acid/protein and cholesterol metabolism and in differences in the expressions of genes related to carbohydrate metabolism. Information gained through this study opens up avenues for further reduction of marine ingredients in trout diets, including the whole rearing cycle.Key words: Fish: Nutrition: Plant products: Fatty acids: Gene expression: Microarrays: Early stages Aquaculture is currently supplying increasing proportions of fish for global human consumption, resulting in an increasing demand for feeds for farmed fish. The use of fishmeal (FM) and fish oil (FO) in fish nutrition, particularly for carnivorous species such as salmonids, has been common practice for years. This is due to the fact that FM and FO constitute excellent sources of essential amino acids and fatty acids, particularly highly unsaturated fatty acids (1)(2)(3) . However, the current stagnation of FM and FO production from wild fisheries might limit the growth of aquaculture unless effective alternative ingredients are found.Terrestrial plant-based products are thus nowadays increasingly used as substitutes for marine resources in feeds for farmed fish (3,4) . Studies conducted with diets containing little or no FM and high levels of plant protein sources have shown lower growth performance in rainbow trout, possibly linked to reduced feed intake (5) . With regard to dietary FO replacement, several studies carried out in salmonids (6,7) have shown that complete replacement of FO in the diet by vegetable oils does not affect growth or feed efficiency when the n-3 ...

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“…156 A aplicação dessas novas áreas pode ser comprovada pela extensa compilação de trabalhos encontrados na literatura. [157][158][159][160][161][162] A nutrimetabolômica, que se refere à aplicação da metabolômica na área de nutrição, permite a caracterização de fenótipos individuais, por meio da medida de centenas ou milhares de metabólitos, após a exposição a uma dieta específica, por exemplo. De um modo geral, a nutrimetabolômica está focada na descoberta de biomarcadores por meio da avaliação do efeito de uma dieta, 163 estudos de intervenção de dieta em uma rota metabólica, 164,165 e estudo da relação dieta-doença 157 ou, mais recentemente, na busca de uma dieta personalizada.…”

Section: Foodomics E Nutriçãounclassified

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

Canuto¹,

Costa²,

Cruz³

et al. 2017

Quim. Nova

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METABOLOMICS: DEFINITIONS, STATE-OF-THE-ART AND REPRESENTATIVE APPLICATIONS.Metabolomics is an emerging and promising omics approach used to understand biological mechanisms. By untargeted and targeted metabolomics analyses, metabolites are determined in biological samples (fluids, cells, tissues, etc.) by comparison of control groups with altered groups, undergoing different therapies, submitted to differing stress levels, dietary modulation, or promoted by a disease, or specific condition, etc., using sophisticated analytical techniques, and advanced data treatment and statistical analyses. In this review, the concepts involved in metabolomics studies were presented, describing in details all steps involved in the metabolomics workflow, for untargeted and targeted strategies. Finally, the potential of metabolomics is illustrated by applications in representative areas: clinical, environmental, food and nutrition, forensic toxicology, microbiology, parasitology, plants, and sports. Relevant reviews were compiled to characterize each of these areas, and a corresponding application of untargeted and targeted metabolomics were described.Keywords: metabolomics; untargeted metabolomics; targeted metabolomics; metabolomics workflow. INTRODUÇÃOAs ciências ômicas buscam o entendimento do funcionamento celular dos organismos e suas alterações biológicas. Fazem parte deste conjunto de ciências, a genômica (estudo da alteração dos genes), a transcriptômica (estudo das alterações dos transcritos), a proteômica (estudo das alterações das proteínas), e a metabolômica (estudo das alterações dos metabólitos). Metabólitos são produtos intermediários ou finais do metabolismo em uma amostra biológica.1 O conjunto de todos os metabólitos de baixa massa molecular (até 1500 Da), presentes ou alterados em um sistema biológico, é chamado de metaboloma (do inglês, metabolome).2 A pesquisa relacionada a metabólitos vem sendo desenvolvida há décadas, mas em 1999, Nicholson et al. definiram a metabonômica (do inglês, metabonomics), como sendo a medida quantitativa da resposta metabólica de um sistema biológico após estímulos fisiopatológicos ou modificações genéticas.3 Já o termo metabolômica (do inglês, metabolomics) foi introduzido em 2001, por Oliver Fiehn, como sendo a análise abrangente e quantitativa do metaboloma de um sistema biológico. 4 Além disso, a literatura nos apresenta outras denominações envolvendo esse campo da ciência, tais como: perfil metabólico (do inglês, metabolic profiling), descrito como sendo a análise de metabólitos previamente selecionados de rotas bioquímicas específicas, 5 a impressão digital metabólica (do inglês, metabolic fingerprinting), que é definida como sendo "uma classificação de amostras de acordo com sua origem ou sua relevância biológica", 3 e, por último, análise footprinting (ainda sem tradução para o português), para se referir aos metabólitos excretados por uma célula em condições controladas. Observa-se, portanto, uma divergência em relação às terminologias aplicadas às análises metabolômic...

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Application of nutrigenomics tools in animal feeding and nutritional research

Cited by 18 publications

References 43 publications

Growth performance and expression of immune-regulatory genes in rainbow trout (Oncorhynchus mykiss) juveniles fed extruded diets with varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus)

Growth performance and expression of immune-regulatory genes in rainbow trout (Oncorhynchus mykiss) juveniles fed extruded diets with varying levels of lupin (Lupinus albus), peas (Pisum sativum) and rapeseed (Brassica napus)

Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins

Metabolômica: Definições, Estado-Da-Arte E Aplicações Representativas

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