Application of novel lytic bacteriophages to control Vibrio parahaemolyticus load in seafood

Dechamma, Mundanda Muthappa; Santhosh, K S; Maiti, Biswajit; Karunasagar, Iddya

doi:10.1007/s00003-021-01356-8

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…However, some chemotherapeutics are not advised because of their severe negative impacts on human, animal, and environmental health (Austin, 2017; A. Kim et al, 2018). Thus, it is necessary to investigate the wide range of permissible antibiotics as well as to develop alternative smart approaches to eliminate the adverse effects of V. parahaemolyticus AHPND, using host‐associated probiotics or the competitive activity of bacteriophages (e.g., Cao et al, 2021; Dechamma et al, 2022; T. L. Nguyen et al, 2018; Pereira et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Multi‐antibiotics resistance phenotype of pathogenic Vibrio parahaemolyticus isolated from acute hepatopancreatic necrosis disease in Litopenaeus vannamei farmed in the Mekong Delta

Thi

Thuy

et al. 2023

J World Aquaculture Soc

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Vibrio parahaemolyticus is a significant causal agent of acute hepatopancreatic necrosis disease (AHPND), with huge production losses of white leg shrimp, Litopenaeus vannamei, cultivated globally, including aquaculture farms in the Mekong Delta of Vietnam. Controlling this disease is critical because of the worldwide expansion of antimicrobial‐resistant V. parahaemolyticus isolates. The purpose of this study was to determine the frequency of multi‐antibiotic resistance (MAR) in V. parahaemolyticus isolated from AHPND white leg shrimp extensively cultivated in Bac Lieu Province (Mekong Delta). Based on biochemical tests and toxR‐PCR positive detections, 34 V. parahaemolyticus isolates were identified. Antibiotic susceptibility examination revealed that most of the isolates were phenotypically multidrug resistant, including resistance to ceftazidime (100%) and amoxicillin (97.06%), followed by colistin (74%) and erythromycin (65%). These isolates were highly sensitive to doxycycline (94%), followed by florfenicol (74%) and flumequine (71%). There were 27 distinct MAR phenotypes detected among 34 isolates, with 14.71% of the isolates exhibiting the antibiotic profile AMO‐CEF‐CEP‐COL‐ERY, followed by 11.76% for each of the profiles AMO‐CEP‐TET‐COL and AMO–CEP–COL. In particular, 25 isolates (75%) were resistant to at least 5 (of the 12) antibiotics tested. A hierarchical clustering analysis of antibiotic‐resistant V. parahaemolyticus isolates indicated cross‐transmission across farms and its ability to survive in aquatic environments for extended periods (over 3 months). Our findings support the hypothesis that the variety of MARPs in V. parahaemolyticus is the result of human activities. Environmentally friendly therapy strategies should be used for the prophylaxis and treatment of V. parahaemolyticus infection.

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Section: Discussionmentioning

confidence: 99%

Multi‐antibiotics resistance phenotype of pathogenic Vibrio parahaemolyticus isolated from acute hepatopancreatic necrosis disease in Litopenaeus vannamei farmed in the Mekong Delta

Thi

Thuy

et al. 2023

J World Aquaculture Soc

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“…They are also a part of the human microbiota, and thereby possess a high level of safety. Thus, due to their high speci city and safety, phages have emerged as ideal instruments for the control and detection of various foodborne pathogens, showing promising applications [13].…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriophages, specialized viruses of bacteria, play a crucial role in the regulation and control of bacterial populations within the environment [11]. Since their initial discovery, phages have signi cantly in uenced our comprehension of the broader biological realm [12].…”

Section: Introductionmentioning

confidence: 99%

Phage-mediated aggregation of gold nanoparticles for visual detection of Vibrio parahaemolyticus

Li,

Wang,

Yan

et al. 2024

Preprint

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Background Vibrio parahaemolyticus (V. parahaemolyticus) is a foodborne pathogen responsible for bacterial food poisoning, necessitating effective detection strategies. Results In this study, the lytic phage VPP1 was isolated and characterized at the biological and genomic levels. VPP1 displayed specificity for V. parahaemolyticus and stability under changing conditions. Genome sequencing of the phage VPP1 revealed a 42,445 bp DNA genome with 64 open reading frames (ORFs), lacking genes involved in pathogenicity or drug resistance. A colorimetric method for the rapid detection of V. parahaemolyticus was subsequently established using gold nanoparticles (AuNPs) and the phage VPP1 (AuNPs@VPP1). The aggregation of AuNPs was mediated by the phage VPP1 upon capturing targeted V. parahaemolyticus. The detection process could be completed within 75 minutes, enabling qualitative detection using the naked eye and quantitative detection using a UV-Vis spectrophotometer. The method enabled the detection of the targeted V. parahaemolyticus within the range of 8.9×101 to 8.9×108 CFU/mL, without noticeable cross-reaction with interfering or dead bacteria. The recoveries of V. parahaemolyticus in spiked lake water and crayfish samples were 81.07–129.17% and 83.78–129.73%, respectively. Conclusions This study indicated that phage-mediated aggregation of AuNPs holds promise for rapid and specific detection of pathogens in food.

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Vibrio parahaemolyticus in seafood: recent progress in understanding influential factors at harvest and food-safety intervention approaches

Ndraha¹,

Huang²,

Wu³

et al. 2022

Current Opinion in Food Science

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Application of novel lytic bacteriophages to control Vibrio parahaemolyticus load in seafood

Cited by 3 publications

References 39 publications

Multi‐antibiotics resistance phenotype of pathogenic Vibrio parahaemolyticus isolated from acute hepatopancreatic necrosis disease in Litopenaeus vannamei farmed in the Mekong Delta

Multi‐antibiotics resistance phenotype of pathogenic Vibrio parahaemolyticus isolated from acute hepatopancreatic necrosis disease in Litopenaeus vannamei farmed in the Mekong Delta

Phage-mediated aggregation of gold nanoparticles for visual detection of Vibrio parahaemolyticus

Vibrio parahaemolyticus in seafood: recent progress in understanding influential factors at harvest and food-safety intervention approaches

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