Application of novel consortium TSR for treatment of industrial dye manufacturing effluent with concurrent removal of ADMI, COD, heavy metals and toxicity

Abstract

The present study was aimed towards the effective bio-treatment of actual industrial effluent containing as high as 42,000 mg/L COD (chemical oxygen demand), >28,000 ADMI (American Dye Manufacturers Institute) color value and four heavy metals using indigenous developed bacterial consortium TSR. Mineral salt medium supplemented with as low as 0.02% (w/v) yeast extract and glucose was found to remove 70% ADMI, 69% COD and >99% sorption of heavy metals in 24 h from the effluent by consortium TSR. The biodegradation of effluent was monitored by UV-vis light, HPLC (high performance liquid chromatography), HPTLC (high performance thin layer chromotography) and FTIR (Fourier transform infrared spectroscopy) and showed significant differences in spectra of untreated and treated effluent, confirming degradation of the effluent. Induction of intracellular azoreductase (107%) and NADH-DCIP reductase (128%) in addition to extracellular laccase (489%) indicates the vital role of the consortium TSR in the degradation process. Toxicity study of the effluent using Allium cepa by single cell gel electrophoresis showed detoxification of the effluent. Ninety per cent germination of plant seeds, Triticum aestivum and Phaseolus mungo, was achieved after treatment by consortium TSR in contrast to only 20% and 30% germination of the respective plants in case of untreated effluent.

Genome analysis to decipher syntrophy in the bacterial consortium ‘SCP’ for azo dye degradation

“…The social intellect of the cultures in a community further depends on the metabolite-mediated cross-talks [ 12 ]. Many scientific groups have worked on the development of a consortium or mixed culture for the bioremediation of reactive azo dyes [ 13 – 15 ]. However, the complexities underlying the group of microorganisms during the bioremediation of dyes have not been well-understood.…”

Bioassays based on higher plants as excellent dosimeters for ecotoxicity monitoring: A review

“…Another study also confirmed the A. cepa test efficiency for toxicity monitroring of Ganoderma lucidum IBL-05 crude ligninolytic enzymes (MnP LiP and Laccase) treated textile effluents (Bilal et al, 2016f). Patel et al (2015) also evaluated the efficiency of A. cepa to monitor the effectiveness of biotreatment of industrial effluent. Toxicity study of the effluent using A. cepa revealed that detoxification of the effluent as a result of bio-treatment was significant.…”

“…For example, mineral salt medium supplemented with as low as 0.02% (w/v) yeast extract and glucose was found to remove 70% ADMI, 69% COD and >99% sorption of heavy metals in 24 h from the effluent by consortium TSR. Toxicity study of the effluent showed 90% germination, which was 20%-30% of the untreated effluent for A. cepa along with other plant species (Patel et al, 2015). Olorunfemi et al (Olorunfemi et al, 2015c) evaluated bilge water (water from oceanic vessels is usually discharged through the bilge wells) for toxicity using A. cepa test from Nigerian marine environment.…”

Decolourization, Degradation and Detoxification of Dye House Effluents by a Developed Bacterial Consortium

“…Dye house effluents (DHEs) generally contain dark coloured water along with heavy metals (chromium, copper, molybdenum, zinc, etc. ), COD, BOD, TOC, TDS and TSS (Patel et al, 2015). Such metal containing pollutants are known to be hazarus for living beings (Li et al, 2015).…”

“…Such metal containing pollutants are known to be hazarus for living beings (Li et al, 2015). Moreover, some portion of dyes are not recovered during the downstream processing and hence dye house wastes are more concentrated as compared to the textile wastewater because textile waste waters get diluted during the washing process of clothes (Patel et al, 2015). Physical and chemical treatment methods are not always possible as they are more costly and practically ineffective.…”