Application of normal- and reversed-phase 2D TLC on a cyanopropyl-bonded polar stationary phase for separation of phenolic compounds from the flowers of<i>Sambucus nigra</i>L.

Hawrył, Mirosław; Hawrył, Anna; Soczewiński, Edward

doi:10.1556/jpc.15.2002.1.1

Cited by 34 publications

(11 citation statements)

References 22 publications

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“…Two-dimensional TLC on cyanopropyl-bonded silica method was employed to separate eight flavonoids and three phenolic acids in Flos sambuci [89]. A normal-phase separation was the first dimension where seven binary eluents were tested, and a reversed-phase separation was the second one, studied by using three binary eluents.…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

841

539

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ReviewExtraction, separation, and detection methods for phenolic acids and flavonoidsThe impetus for developing analytical methods for phenolic compounds in natural products has proved to be multifaceted. Hundreds of publications on the analysis of this category of compounds have appeared over the past two decades. Traditional and more advanced techniques have come to prominence for sample preparation, separation, detection, and identification. This review provides an updated and extensive overview of methods and their applications in natural product matrices and samples of biological origin. In addition, it critically appraises recent developments and trends, and provides selected representative bibliographic examples.

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Section: Thin-layer Chromatographymentioning

confidence: 99%

Extraction, separation, and detection methods for phenolic acids and flavonoids

Stalikas¹

2007

J of Separation Science

841

539

View full text Add to dashboard Cite

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“…The content of phenolic compounds in biological samples can be determined by various analytical instrumental methods, such as gas chromatography [14], thin-layer chromatography [15], and capillary electrophoresis [16]. However, high performance liquid chromatography (HPLC) has proved to be the most appropriate owing to the structural similarity and diversity of phenolic compounds, allowing the analysis with sufficient precision, selectivity and within a reasonable time.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection

Zhang

Wan

et al. 2013

Molecules

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Abstract:The paper described a novel chromatographic method for the simultaneous determination of phenolic compounds such as gallic, protocatechuic, vanillic, caffeic, syringic, p-coumaric and salicylic acid, (+)-catechin, (-)-epicatechin, rutin, morin, quercetin, coumarin and trans-resveratrol at their maximum absorbance wavelengths (MAW) employing reverse-phase high performance liquid chromatography combined with DAD and UV detection via detection wavelength switching. The method was based on MAW acquisition by DAD and quantification by UV. The separation process was performed on a Shim-Pack VP-ODS C 18 column (250 mm × 4.6 mm, 5 μm) held at 30 °C, utilizing 3.0% acetic acid and acetonitrile as mobile phase at a flow rate of 0.8 mL/min in the gradient elution mode. The method was fully validated in terms of linearity (r 2 > 0.9990, 10-350 mg/L), precision (both intra-day and inter-day RSD < 4.22%), accuracy (97.31%-104.66%), specificity, robustness (0.59% < RSD < 2.86%), limit of detection and quantification. The switching method significantly improved the sensitivities of most phenolics studied in comparison with the standard constant wavelength detection (280 nm). OPEN ACCESSMolecules 2013, 18 14242The proposed method has been successfully applied to the determination of 14 phenolic compounds in 89 varieties of one-year-old Chinese grape one-year-canes. Grape canes contain many phenolics, especially trans-resveratrol, (-)-epicatechin, and (+)-catechin.

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“…The health benefits and diverse commercial applications of phenolic compounds have prompted the interest of the scientists to increase their concentrations in plant by using different techniques such as genetic engineering [15]. The separation and quantification of phenolic compound has been widely reported previously by several research groups using analytical techniques such as U/HPLC, GC, TLC and CE [16][17][18]. Pakistan is a major producer and exporter of many high value rice varieties.…”

Section: Introductionmentioning

confidence: 99%

Determination of Important Phenolic Compounds in Pakistani Brown Rice Varieties in Controlled, Germinated and Fermented Conditions by High Performance Liquid Chromatography

2019

Prog. Chem. Biochem. Res.

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A simple and novel high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination and quantification of phenolic compounds in brown whole grain rice varieties of Pakistani origin. The accumulation of these compounds was studied during germination stages and fermentation process, thereby, providing a reliable and rapid method for their quantification in food samples. Calibration curves for the standard phenolic compounds showed good linear regression values (r 2 =0.996-0.998) within the test ranges. The limit of detection and the limit of quantification were found in the range of 0.04-0.06 µg/mL and 0.166-0.205µg/mL, respectively. Precision (%RSD) of the method was found in the range of 0.05-5.25 and 0.05-0.58 for inter-day (n=3) and intra-day (n=5), respectively. The robustness (%RSD) was found in the range of 1.05-2.65. Excellent recoveries were attained within the range of 93%-106%.The average amount of phenolic compounds was found to be 0.185 g/100g in controlled condition whereas, further accumulation of these compounds was noticed during germination and fermentation phases. The maximum average amount of phenolic compounds after germination period of 120 hours and fermentation process was found to be 0.284g/100gand 0.565 g/100g, respectively.

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Application of normal- and reversed-phase 2D TLC on a cyanopropyl-bonded polar stationary phase for separation of phenolic compounds from the flowers ofSambucus nigraL.

Cited by 34 publications

References 22 publications

Extraction, separation, and detection methods for phenolic acids and flavonoids

Extraction, separation, and detection methods for phenolic acids and flavonoids

Simultaneous Determination of 14 Phenolic Compounds in Grape Canes by HPLC-DAD-UV Using Wavelength Switching Detection

Determination of Important Phenolic Compounds in Pakistani Brown Rice Varieties in Controlled, Germinated and Fermented Conditions by High Performance Liquid Chromatography

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