Application of Nextera™ technology to RNA-seq library preparation

Syed, Fraz

doi:10.1038/nmeth.f.317

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Next Generation Library Preparation1

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Cited by 4 publications

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“…A newer innovation involves transposonmediated fragmentation and adaptor ligation, processes originally designed for DNA sequencing that can be adapted for RNA-Seq library preparation (Syed 2010;Gertz et al 2012). Unlike previous RNA-Seq preparation methods, in which polyadenylated RNA ( polyA RNA) was selected and fragmented, then converted to double-stranded cDNA (ds cDNA), the new method involves a polyA RNA selection step during first-strand cDNA synthesis followed by transposon-based fragmentation of ds cDNA.…”

Section: Next Generation Library Preparationmentioning

confidence: 99%