Application of Next-Generation Sequencing in Neurodegenerative Diseases: Opportunities and Challenges

Shademan, Behrouz; Avcı, Çığır Biray; Nikanfar, Masoud; Nourazarian, Alireza

doi:10.1007/s12017-020-08601-7

Cited by 11 publications

(14 citation statements)

References 104 publications

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“…Targeted Sequencing is restricted to a specific set of genes or genomic regions ( Rizzo and Buck, 2012 ; Hu et al, 2021 ) to increase sequencing depth in order to focus on biological regions implicated in human disorders. These approaches allow for identification of genetic mutations and epigenetic regulation that may underly psychiatric and neurological disorders [for more detailed reviews: ( Rizzo and Buck, 2012 ; Shin et al, 2014 ; Shademan et al, 2021 )].…”

Section: Advances In Genomic Technology For Transcriptomic Translatom...mentioning

confidence: 99%

Advancements in Genomic and Behavioral Neuroscience Analysis for the Study of Normal and Pathological Brain Function

Baratta

Brandner²,

Plasil³

et al. 2022

Front. Mol. Neurosci.

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Psychiatric and neurological disorders are influenced by an undetermined number of genes and molecular pathways that may differ among afflicted individuals. Functionally testing and characterizing biological systems is essential to discovering the interrelationship among candidate genes and understanding the neurobiology of behavior. Recent advancements in genetic, genomic, and behavioral approaches are revolutionizing modern neuroscience. Although these tools are often used separately for independent experiments, combining these areas of research will provide a viable avenue for multidimensional studies on the brain. Herein we will briefly review some of the available tools that have been developed for characterizing novel cellular and animal models of human disease. A major challenge will be openly sharing resources and datasets to effectively integrate seemingly disparate types of information and how these systems impact human disorders. However, as these emerging technologies continue to be developed and adopted by the scientific community, they will bring about unprecedented opportunities in our understanding of molecular neuroscience and behavior.

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Section: Advances In Genomic Technology For Transcriptomic Translatom...mentioning

confidence: 99%

Advancements in Genomic and Behavioral Neuroscience Analysis for the Study of Normal and Pathological Brain Function

Baratta

Brandner²,

Plasil³

et al. 2022

Front. Mol. Neurosci.

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show abstract

“…It is also characterized by attacks by the immune system on a specific protective layer (myelin) surrounding neurons, resulting in sensory‐motor dysfunction. Ultimately, the disease may lead to degeneration or irreversible loss of the affected nerves 2 . Despite the extensive research that has been conducted on MS, the exact etiology of the disease remains unclear 3,4 …”

Section: Introductionmentioning

confidence: 99%

“…It has been found that MS is associated with excessive T‐cell reactivity to various antigens and also with abnormal B‐cell responses 1,2 . Given the diversity of neurological impairments in different patients, the disease may manifest with different clinical presentations.…”

Section: Introductionmentioning

confidence: 99%

“…Ultimately, the disease may lead to degeneration or irreversible loss of the affected nerves. 2 Despite the extensive research that has been conducted on MS, the exact etiology of the disease remains unclear. 3 , 4…”

Section: Introductionmentioning

confidence: 99%

“…It has been found that MS is associated with excessive T‐cell reactivity to various antigens and also with abnormal B‐cell responses. 1 , 2 Given the diversity of neurological impairments in different patients, the disease may manifest with different clinical presentations. The risk of developing MS is higher in individuals with a family history of autoimmune disease and those with specific risk factors for the disease, including obesity, Epstein‐Barr virus infection, vitamin D deficiency, smoking, and lack of sun exposure.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

Moharami

Nourazarian

Nikanfar

et al. 2021

Clinical Laboratory Analysis

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Background Multiple sclerosis (MS) is a chronic inflammatory and autoimmune disease affecting various inflammatory and nutritional parameters. Therefore, this study aimed to investigate the relationship between the Body Mass Index (BMI) of MS patients and the serum levels of leptin, orexin‐A, and Transforming Growth Factor β (TGF‐β). Methods This cross‐sectional study included 25 patients suffering from MS and 40 healthy individuals as the case and control groups, respectively. The serum levels of leptin, orexin‐A, and TGF‐β were assessed in the participants using the Enzyme‐Linked Immunosorbent Assay methods. Moreover, data were analyzed using the descriptive statistical indices, t‐test, chi‐square test, and linear regression test. Results According to our results, the participants’ mean age was 38.04 ± 7.53 and 40.23 ± 5.88 in the case and control groups, respectively. Also, the groups were not significantly different in gender, age, alcohol consumption, and smoking (p > 0.05). It was found that the mean serum levels of orexin‐A and TGF‐β were significantly lower in the MS patients compared to the control group, while the mean serum leptin levels were significantly higher (42.8 vs. 18.9 ng/ml, p < 0.001). Moreover, there was no significant relationship between the BMI of the MS patients and their serum levels of orexin‐A, TGF‐β, and leptin (p > 0.05). Conclusions In conclusion, we found significantly lower levels of orexin‐A and TGF‐β and a significantly higher level of leptin in the MS patients compared to the control group. In addition, there was no significant relationship between the BMI and the serum levels of orexin‐A, TGF‐β, and leptin in MS patients.

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A review of migraine genetics: gathering genomic and transcriptomic factors

et al. 2021

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Application of Next-Generation Sequencing in Neurodegenerative Diseases: Opportunities and Challenges

Cited by 11 publications

References 104 publications

Advancements in Genomic and Behavioral Neuroscience Analysis for the Study of Normal and Pathological Brain Function

Advancements in Genomic and Behavioral Neuroscience Analysis for the Study of Normal and Pathological Brain Function

Investigation of serum levels of orexin‐A, transforming growth factor β, and leptin in patients with multiple sclerosis

A review of migraine genetics: gathering genomic and transcriptomic factors

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