Application of near infrared (NIR) spectroscopy for determining the thickness of articular cartilage

Afara, Isaac O.; Singh, Samarendra P.; Oloyede, Adekunle

doi:10.1016/j.medengphy.2012.04.003

Cited by 49 publications

(67 citation statements)

References 34 publications

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“…Recently, NIR has been used to develop a non-destructive method to determine the thickness of cartilage 25 . In that study, NIR spectra were collected from 1.2 mm to 2.4 mm thick cartilage samples from bovine samples.…”

Section: Discussionmentioning

confidence: 99%

Wavelength-dependent penetration depth of near infrared radiation into cartilage

Padalkar

Pleshko

2015

Analyst

118

109

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Articular cartilage is a hyaline cartilage that lines the subchondral bone in a diarthrodial joint. Near infrared (NIR) spectroscopy has been emerging as a nondestructive modality for evaluation of cartilage pathology. However, studies of the depth of penetration of NIR radiation into cartilage are lacking. The average thickness of human cartilage is about 1-3 mm, and it becomes even thinner as OA progresses. To ensure that the spectral data collected is restricted to the tissue of interest i.e. cartilage in this case, and not from the underlying subchondral bone, it is necessary to determine the depth of penetration of NIR radiation in different wavelength (frequency) regions. In the current study we establish how the depth of penetration varies throughout the NIR frequency range (4000-10000 cm-1). NIR spectra were collected from cartilage samples of different thicknesses (0.5 mm to 5 mm) with and without polystyrene placed underneath. A separate NIR spectrum of polystyrene was collected as a reference. It was found that the depth of penetration varied from ∼1 mm to 2 mm in the 4000-5100 cm-1 range, to ∼3 mm in the 5100-7000 cm-1 range, and to ∼5 mm in the 7000-9000 cm-1 frequency range. These findings suggest that the best NIR region to evaluate cartilage only with no subchondral bone contribution is between 4000-7000 cm-1.

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Section: Discussionmentioning

confidence: 99%

Wavelength-dependent penetration depth of near infrared radiation into cartilage

Padalkar

Pleshko

2015

Analyst

118

109

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“…The PLS model predicted collagen and chondroitin sulfate content of samples to within 8% of values obtained by biochemical assessment. Previous studies have incorporated the contribution of water in cartilage sample assessment (Spahn et al, 2007(Spahn et al, , 2008Brown et al, 2009;Baykal et al, 2010;Afara et al, 2012Afara et al, , 2013aAfara et al, , 2013bPadalkar et al, 2013;Spahn et al, 2013a); this was not addressed in this study as the absorption bands of water have higher signal intensity and often overwhelm the contributions from the other components of cartilage tissue. Thus, the goal here was to utilize a spectral range with limited water contribution.…”

Section: Discussionmentioning

confidence: 99%

“…However, the absorbances in NIR spectra are very low, and generally arise from a combination of molecular species, making assignments of specific peaks challenging. Nevertheless, several studies have demonstrated the effectiveness of NIR spectroscopy in evaluating cartilage tissue quality non-destructively (Spahn et al, 2007(Spahn et al, , 2008Brown et al, 2009;Baykal et al, 2010;Afara et al, 2012Afara et al, , 2013aAfara et al, , 2013bAfara et al, , 2013cPadalkar et al, 2013). Studies have also shown that the NIR spectra obtained using an IFOP can be used to monitor disease progression in OA cartilage, and that this modality can perform better than other clinically used procedures to evaluate cartilage degeneration (Hofmann et al, 2010;Brown et al, 2011Brown et al, , 2012Spahn et al, 2013b).…”

Section: Introductionmentioning

confidence: 99%

Assessment of hyaline cartilage matrix composition using near infrared spectroscopy

2014

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Changes in the composition of the extracellular matrix (ECM) are characteristic of injury or disease in cartilage tissue. Various imaging modalities and biochemical techniques have been used to assess the changes in cartilage tissue but lack adequate sensitivity, or in the case of biochemical techniques, result in destruction of the sample. Fourier transform near infrared (FT-NIR) spectroscopy has shown promise for the study of cartilage composition. In the current study NIR spectroscopy was used to identify the contributions of individual components of cartilage in the NIR spectra by assessment of the major cartilage components, collagen and chondroitin sulfate, in pure component mixtures. The NIR spectra were obtained using homogenous pellets made by dilution with potassium bromide. A partial least squares (PLS) model was calculated to predict composition in bovine cartilage samples. Characteristic absorbance peaks between 4000 and 5000 cm(-1) could be attributed to components of cartilage, i.e. collagen and chondroitin sulfate. Prediction of the amount of collagen and chondroitin sulfate in tissues was possible within 8% (w/dw) of values obtained by gold standard biochemical assessment. These results support the use of NIR spectroscopy for in vitro and in vivo applications to assess matrix composition of cartilage tissues, especially when tissue destruction should be avoided.

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“…The weight of the glass slide alone was subtracted, and for each time point during the course of evaporation of water from the tissues, the gravimetrically-determined absolute water content was calculated as: (1) Where, Wet weight T = the tissue weight at a specific time point T Dry weight L = the weight of the tissue after lyophilization (2) …”

Section: Data Analysis: Bnc Water Contentmentioning

confidence: 99%

Near infrared spectroscopic evaluation of water in hyaline cartilage

Padalkar

Spencer

Pleshko

2012

2012 38th Annual Northeast Bioengineering Conference (NEBEC)

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In diseased conditions of cartilage such as osteoarthritis, there is typically an increase in water content from the average normal of 60-85% to greater than 90%. As cartilage has very little capability for self-repair, methods of early detection of degeneration are required, and assessment of water could prove to be a useful diagnostic method. Current assessment methods are either destructive, time consuming or have limited sensitivity. Here, we investigated the hypotheses that non-destructive near infrared spectroscopy (NIRS) of articular cartilage can be used to differentiate between free and bound water, and to quantitatively assess water content. The absorbances centered at 5200 cm −1 and 6890 cm −1 were attributed to a combination of free and bound water, and to free water only, respectively. The integrated areas of both absorbance bands were found to correlate linearly with the absolute water content (R=0.87 and R= 0.86) and with percent water content (R=0.97 and R=0.96) of the tissue. Partial least square models were also successfully developed and were used to predict water content, and percent free water. These data demonstrate that NIRS can be utilized to quantitatively determine water content in articular cartilage, and may aid in early detection of degenerative tissue changes in a laboratory setting, and with additional validations, possibly in a clinical setting.

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Application of near infrared (NIR) spectroscopy for determining the thickness of articular cartilage

Cited by 49 publications

References 34 publications

Wavelength-dependent penetration depth of near infrared radiation into cartilage

Wavelength-dependent penetration depth of near infrared radiation into cartilage

Assessment of hyaline cartilage matrix composition using near infrared spectroscopy

Near infrared spectroscopic evaluation of water in hyaline cartilage

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