Application of Nanobiotechnology in Cancer Therapeutics

Jain, Kewal K.

doi:10.1007/978-1-4419-0131-6_8

Cited by 6 publications

(5 citation statements)

References 56 publications

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“…Localization at the level of mitochondria is realized by the presence of extra nuclear DNA [25]. These data are consistent with other experiments which underline that silver nanoparticles penetrate the cells membrane and can be used for the detection of the SERS signal [26,27]. Their aggregation is possible in the growth medium and due to their increased size endocytosis is assumed [28].…”

Section: Resultssupporting

confidence: 83%

A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior

et al. 2013

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BackgroundOne of the most popular and versatile model of murine melanoma is by inoculating B16 cells in the syngeneic C57BL6J mouse strain. A characterization of different B16 modified cell sub-lines will be of real practical interest. For this aim, modern analytical tools like surface enhanced Raman spectroscopy/scattering (SERS) and MTT were employed to characterize both chemical composition and proliferation behavior of the selected cells.MethodsHigh quality SERS signal was recorded from each of the four types of B16 cell sub-lines: B164A5, B16GMCSF, B16FLT3, B16F10, in order to observe the differences between a parent cell line (B164A5) and other derived B16 cell sub-lines. Cells were incubated with silver nanoparticles of 50–100 nm diameter and the nanoparticles uptake inside the cells cytoplasm was proved by transmission electron microscopy (TEM) investigations. In order to characterize proliferation, growth curves of the four B16 cell lines, using different cell numbers and FCS concentration were obtained employing the MTT proliferation assay. For correlations doubling time were calculated.ResultsSERS bands allowed the identification inside the cells of the main bio-molecular components such as: proteins, nucleic acids, and lipids. An "on and off" SERS effect was constantly present, which may be explained in terms of the employed laser power, as well as the possible different orientations of the adsorbed species in the cells in respect to the Ag nanoparticles. MTT results showed that among the four tested cell sub-lines B16 F10 is the most proliferative and B164A5 has the lower growth capacity. Regarding B16FLT3 cells and B16GMCSF cells, they present proliferation ability in between with slight slower potency for B16GMCSF cells.ConclusionMolecular fingerprint and proliferation behavior of four B16 melanoma cell sub-lines were elucidated by associating SERS investigations with MTT proliferation assay.

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Section: Resultssupporting

confidence: 83%

A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior

et al. 2013

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“…The attributes of organic and inorganic nanoparticles, such as small size, composition, surface structure, solubility, shape and aggregation allow for unlimited modifications of their basic properties such as diffusivity, targeting, stability, solubility, half-life in circulatory system and controlled drug release. Due to such unique features, nanoparticles have the advantage over traditional therapeutic and diagnostic agents [1][2][3][4]. Nanoresearch is focused on many life-sciences including applications in environmental, health and safety sciences.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Jennifer¹,

Wnuk²

2013

JBNB

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Nanoparticles are considered as powerful tools in nanotechnological applications. Due to their unique physicochemical properties, their interactions with different biological systems have been shown. Nanomaterials have been successfully used as coating materials or treatment and diagnosis tools. Nevertheless, toxic effects of nanoparticles in vitro and in vivo have also been reported. Here, we summarize the current state of knowledge on exposure routes, cellular uptake and toxicological activities of the commonly used nanoparticles. In this context, we discuss the mechanisms of toxicity of nanoparticles involving perturbation of redox milieu homeostasis and cellular signaling pathways.

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“…Thus, it can be expected that an ideal nanoparticular delivery system that can significantly overcome MDR would be dependent on the high efficiency of cellular entry of the nanoparticles and subsequent rapid release of the cytotoxic drug intracellularly that should be sufficiently high to induce cytotoxicity. Gold nanoparticles (AuNPs), which are known to be inert, have shown advantages and significant progress in the delivery of small molecules to large biomacromolecules, including DNA, siRNA, and proteins. − On the other hand, recent studies have indicated that AuNPs act as good quenchers of many fluorescence donors, due to the nanosurface energy transfer (NSET) effect. − This may render AuNPs as the base of a fluorescent “nanoprobe”, particularly useful in the trafficking of intracellular drug release, by monitoring changes in fluorescence. In this study, we developed a drug delivery system by tethering doxorubicin onto the surface of AuNPs with a poly(ethylene glycol) spacer via an acid-labile linkage (Scheme ), which can significantly overcome P-gp-mediated drug resistance by a combination of enhanced doxorubicin cellular entry and a responsive intracellular release of doxorubicin in acidic organelles.…”

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confidence: 99%

Doxorubicin-Tethered Responsive Gold Nanoparticles Facilitate Intracellular Drug Delivery for Overcoming Multidrug Resistance in Cancer Cells

et al. 2011

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Multidrug resistance (MDR) is a major impediment to the success of cancer chemotherapy. Through the development of a drug delivery system that tethers doxorubicin onto the surface of gold nanoparticles with a poly(ethylene glycol) spacer via an acid-labile linkage (DOX-Hyd@AuNPs), we have demonstrated that multidrug resistance in cancer cells can be significantly overcome by a combination of highly efficient cellular entry and a responsive intracellular release of doxorubicin from the gold nanoparticles in acidic organelles. DOX-Hyd@AuNPs achieved enhanced drug accumulation and retention in multidrug resistant MCF-7/ADR cancer cells when it was compared with free doxorubicin. It released doxorubicin in response to the pH of acidic organelles following endocytosis, opposite to the noneffective drug release from doxorubicin-tethered gold nanoparticles via the carbamate linkage (DOX-Cbm@AuNPs), which was shown by the recovered fluorescence of doxorubicin from quenching due to the nanosurface energy transfer between the doxorubicinyl groups and the gold nanoparticles. DOX-Hyd@AuNPs therefore significantly enhanced the cytotoxicity of doxorubicin and induced elevated apoptosis of MCF-7/ADR cancer cells. With a combined therapeutic potential and ability to probe drug release, DOX-Hyd@AuNPs represent a model with dual roles in overcoming MDR in cancer cells and probing the intracellular release of drug from its delivery system.

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Application of Nanobiotechnology in Cancer Therapeutics

Cited by 6 publications

References 56 publications

A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior

A characterization of four B16 murine melanoma cell sublines molecular fingerprint and proliferation behavior

Nanoparticle Technology as a Double-Edged Sword: Cytotoxic, Genotoxic and Epigenetic Effects on Living Cells

Doxorubicin-Tethered Responsive Gold Nanoparticles Facilitate Intracellular Drug Delivery for Overcoming Multidrug Resistance in Cancer Cells

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