Application of monoliths for bioparticle isolation

Abstract

Monoliths are today probably the most studied chromatographic supports. There are plethora of publications dealing with different aspects of their preparation, characterization, and applications. The reason for this interest is their inherent properties related to their particular structure, like ease of preparation in various volumes, fast analytics at low pressure and room temperature, and high productivity as a consequence of flow-unaffected properties, especially important for isolation of large biological molecules. Because of that, structure of several monoliths was optimized for analytics and purification of biologic nanoparticles like viruses, virus-like particles (VLPs), cells structures, or even intact cells. In this review, some recent applications of monoliths in the field of bioparticle isolation are described and results are discussed in terms of particular monolith properties.

Experimental characterization of the transport phenomena, adsorption, and elution in a protein A affinity monolithic medium

“…Indeed, for these molecules, the available surface for binding is almost exclusively limited to the outer bead surface resulting in a significant reduction in the potential binding capacity. On the other hand, monoliths display a higher accessible surface area for large molecules and hence greater binding capacity [6,7]. Among the large molecules of interest viruses [8][9][10], virus-like particles [11], nucleic acids, and plasmid DNA [12][13][14] have been successfully isolated and purified by several research groups demonstrating the advantages of monolithic supports for these applications.…”

“…Monolithic media are used in several liquid chromatography applications in both preparative and analytical scale [4][5][6]. Similar to packed bead columns, monoliths are porous media characterized by a bimodal pore size distribution.…”

New method for the speciation of ruthenium-based chemotherapeutics in human serum by conjoint liquid chromatography on affinity and anion-exchange monolithic disks

“…Nowadays, these columns can be replaced by monolithic chromatographic supports, whose main characteristics are extreme permeability that allows a very efficient mass transport at low back pressures, good separation efficiency that decreases relatively slowly with increasing flow velocity and separation at high flow rates. Due to such characteristics, monoliths are frequently used in chromatographic separations of biomolecules, organic acids and purification of viruses [28][29][30][31] while their use in speciation analysis is scarce. Examples of successful application of monolithic chromatography in speciation analysis are the investigations on Al speciation in human serum [32,33], Zn in human milk [34], Ni in tea infusions [35], the study of the distribution of cisplatin [36] in serum of cancer patients and the investigation of the kinetics of the interaction and distribution of Ptbased chemotherapeutics with proteins in blood serum [37].…”

Concentration and purification of enterovirus 71 using a weak anion-exchange monolithic column

“…Moreover, the purification protocol is simple and the total time taken can be shortened from several days to a few hours without compromising purity. The method developed from a disk/tube monolithic column can be easily scaled-up to litres for bulk purification of biomolecules [29]. Recently, CIM DEAE monolithic columns have been used for the purification of many viruses and bacteriophages including Tomato mosaic virus [16,30], filamentous potato virus [17], Pseudomonas phage LUZ19 [31], Rubella virus [32], Staphylococcus phage ISP [33], viral vectors such as Canine Adenoviral Vectors [14], and Lentiviral Vectors [34].…”