Application of monolithic chromatographic supports in virus research

Abstract

Key properties of monolithic chromatographic supports, make them suitable for separation and/or concentration of large biomolecules, especially virus particles and viral genomes. One by one, the studies that have been completed so far, contributed to the knowledge that monolith chromatography has hardly any limitation to be applied in virus research. Viruses of different sizes, possessing icosahedral structure and symmetrical morphology, as well as rod-shaped or filamentous viruses with helical structure, even enveloped ones, all of them could be successfully managed by means of monolith chromatography. Same is true for viral genomes, primarily when being distinct from other nucleic acid forms present in a host cell. This review is exclusively focused on viruses. It describes the application of monolith chromatography to different problematics within the virus research field. The reviewed achievements offer new possibilities and trigger new aspects in virology.

Rumen Virus Populations: Technological Advances Enhancing Current Understanding

“…Sequence analysis of rumen sample RNA (metatranscriptomics) has also been used to identify RNA virus populations (Hitch et al, 2019). In addition, alternative technologies are being applied for the isolation of virus particles (for example, chromatography) and enabling the expression of novel viral proteins in vitro (Figura et al, 2011;Krajacic et al, 2017;Altermann et al, 2018). The emerging field of proteomics, is also providing fresh insights into the roles viruses and the proteins produced by viruses, may play within the rumen microbial ecosystem (Solden et al, 2018).…”

Current challenges in biotherapeutic particles manufacturing

“…These membranes take advantage of the affinity properties of sulfated cellulose and the convective flow of membrane absorbers. Monolithic supports are characterized by its very high porosity, with channels size mostly ranging between 1-5 µm, high binding capacity, and dominantly convective mass transport, providing high flow rates, reducing the processing time [113,114]. Hereby, monoliths are of huge interest in this field, being used for the purification of several particles [108,115].…”

Construction of polymer monolithic columns in polypropylene ink‐pen tubes for separation of proteins by cation‐exchange chromatography

“…The high porosity of polymer monoliths enables fast separation and purification of large objects such as biomolecules [1] and viruses [2,3] under high flow rates and convective mass transport [4]. Stainless steel, fused silica capillaries [5], fused silica lined stainless steel [6,7], PEEK [8], and fluoropolymer tubes [9] with internal diameters varying from few tens of micrometers to the conventional 4.6 mm served as supports and molds for the monoliths.…”