Application of monoclonal antibody against granulocytes of scallop Chlamys farreri on granulocytes occurrence at different developmental stages and antigenic cross-reactivity of granulocytes in five other bivalve species

Xing, Jing; Tang, Xiaoqian; Ni, Yuwen; Zhan, Wenbin

doi:10.1016/j.fsi.2013.11.001

Cited by 7 publications

(2 citation statements)

References 27 publications

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“…Additionally, the IIFAT method appears to show intracellular staining, but more research is required to conclusively demonstrate this. These results indicated that the IgMHCµ 1 mAb could be applicable for identifying IgM + cells in Clarias fish as well as in many previously-reported teleost fish [17,22,[39][40][41][42][43][44][45][46]. It could suggest that these catfish possess B lymphocyte populations in their immune systems, as reported in other vertebrates [13,35].…”

Section: Discussionsupporting

confidence: 57%

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish

2020

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Catfish is a commonly-cultivated freshwater fish in Thailand and many Southeast Asian countries. The molecular data obtained for the IgM heavy chain (IgMH) of catfish have been useful for distinguishing monoclonal antibodies (mAbs). A mAb specific to Cμ1 of the IgMH of catfish (IgMHCμ1 mAb) was developed in a rabbit model using sequence information from bighead catfish (Clarias macrocephalus). The IgMHCμ1 mAb strongly recognized the IgM heavy chain of the tested catfish, namely, bighead catfish, African catfish (Clarias gariepinus) and their hybrid (C. macrocephalus × C. gariepinus), in immunological Western blot analysis and competitive ELISAs. Additionally, the IgMHCμ1 mAb successfully recognized IgM+ cells by detecting IgM molecules in both secreted and membrane-bound forms in peripheral blood leukocytes (PBLs). The IgMHCμ1 mAb was further used to quantify the percentage of IgM+ cells among PBLs through flow cytophotometry. The IgM+ cell percentages of healthy bighead catfish, African catfish and their hybrid were 38.0–39.9%, 45.6–53.2%, and 58.7–60.0%, respectively. Furthermore, the IgMHCμ1 mAb showed no cross-reactivity with the IgM of zebrafish. These findings suggest that this mAb can be used as an immunological tool for monitoring the health, immune status, and immune development of cultivated Clarias catfish.

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Section: Discussionsupporting

confidence: 57%

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish

2020

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“…For example, immune functions and apoptotic mechanisms are preferentially activated early in development to increase the resistance against benthic pathogens (Xing et al, 2014). However these processes also aid in the elimination of primitive tissue and debris during M A N U S C R I P T ACCEPTED MANUSCRIPT metamorphosis (Dyrynda et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Predicting growth and mortality of bivalve larvae using gene expression and supervised machine learning

Bassim

Chapman

Tanguy

et al. 2015

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Biology and Ecology of Scallop Larvae

Cragg¹

2016

Developments in Aquaculture and Fisheries Science

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Application of monoclonal antibody against granulocytes of scallop Chlamys farreri on granulocytes occurrence at different developmental stages and antigenic cross-reactivity of granulocytes in five other bivalve species

Cited by 7 publications

References 27 publications

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish

Development of a Monoclonal Antibody Specific to the IgM Heavy Chain of Bighead Catfish (Clarias macrocephalus): A Biomolecular Tool for the Detection and Quantification of IgM Molecules and IgM+ Cells in Clarias Catfish

Predicting growth and mortality of bivalve larvae using gene expression and supervised machine learning

Biology and Ecology of Scallop Larvae

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