Application of molecular methods for the classification and identification of lactic acid bacteria

Schleifer, Karl‐Heinz; Ehrmann, M. A.; Beimfohr, Claudia; Brockmann, Elke; Ludwig, Wolfgang; Amann, Rudolf

doi:10.1016/0958-6946(95)00047-x

Cited by 101 publications

(54 citation statements)

References 29 publications

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“…16S rDNA sequences can be used for speciation by homology to sequences from known bacteria in data bases (Schleifer et al, 1995). The usefulness of this tech nique, however, is dependent on the completeness and accuracy of the databases used for comparison.…”

Section: Resultsmentioning

confidence: 99%

Species-Specific Identification of Commercial Probiotic Strains

Yeung¹,

Sanders²,

Kitts³

et al. 2002

Journal of Dairy Science

117

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Products containing probiotic bacteria are gaining popularity, increasing the importance of their accurate speciation. Unfortunately, studies have suggested that improper labeling of probiotic species is common in com mercial products. Species identification of a bank of com mercial probiotic strains was attempted using partial 16S rDNA sequencing, carbohydrate fermentation anal ysis, and cellular fatty acid methyl ester analysis. Re sults from partial 16S rDNA sequencing indicated dis crepancies between species designations for 26 out of 58 strains tested, including two ATCC Lactobacillus strains. When considering only the commercial strains obtained directly from the manufacturers, 14 of 29 strains carried species designations different from those obtained by partial 16S rDNA sequencing. Strains from six commercial products were species not listed on the label. The discrepancies mainly occurred in Lactobacil lus acidophilus and Lactobacillus casei groups. Carbohy drate fermentation analysis was not sensitive enough to identify species within the L. acidophilus group. Fatty acid methyl ester analysis was found to be variable and inaccurate and is not recommended to identify probi otic lactobacilli.

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Section: Resultsmentioning

confidence: 99%

Species-Specific Identification of Commercial Probiotic Strains

Yeung¹,

Sanders²,

Kitts³

et al. 2002

Journal of Dairy Science

117

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“…However, the characterization of some Lactobacillus to species level by biochemical methods alone is not reliable because of the considerable variations in biochemical attributes between strains currently considered to belong to the same species (Schleifer et al, 1995). In fact, some species are not easily distinguishable in terms of phenotypic characteristics (Coeuret et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Makete

Aiyegoro

Thantsha

2016

Probiotics & Antimicro. Prot.

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“…Moreover, there are certain species that cannot readily be distinguished by phenotypic characters (Schleifer et al, 1995). When fish products have been studied, phenotyping of LAB has resulted in unreliable species identification.…”

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confidence: 99%

Characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping

Lyhs

Björkroth

Korkeala

1999

International Journal of Food Microbiology

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A total of 405 lactic acid bacteria (LAB) isolated from spoiled, vacuumpackaged, salted, sodium nitrite-or potassium nitrate-treated, cold-smoked rainbow trout stored at 4ºC or 8ºC were characterised and identified using a molecular method. The isolates were initially classified according to their restriction endonuclease profiles using HindIII and EcoRI restriction endonucleases and further characterised by rRNA gene restriction patterns (ribotypes). Numerical analysis of these ribopatterns was performed together with 19 reference LAB strain patterns in order to identify the isolates to species level. The strains were divided with HindIII and EcoRI ribopatterns into ten and nine clusters at the similarity level of 65% and 50%, respectively. The Leuconostoc-clusters and the Lb. sakei/Lb. curvatus-clusters formed the two main groups. Only one isolate was identified asLactobacillus plantarum and no Carnobacterium strains were discovered. For both enzymes, the 35 isolates possessing six individual ribotypes and forming five clusters could not be identified further with the reference strains used. The relative proportion of Leuconostoc mesenteroides subsp. mesenteroides was higher in all samples stored at 4ºC. Most of the Leuconostoc citreum were found in the samples stored at 8ºC, and particularly in the nitritetreated samples.

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Application of molecular methods for the classification and identification of lactic acid bacteria

Cited by 101 publications

References 29 publications

Species-Specific Identification of Commercial Probiotic Strains

Species-Specific Identification of Commercial Probiotic Strains

Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats

Characterisation of lactic acid bacteria from spoiled, vacuum-packaged, cold-smoked rainbow trout using ribotyping

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