Application of mixed-mode ultra high performance liquid chromatography to forensic drug analysis

Clyde, Cassandra; Blake, Samantha; O’Brien, Stacey; Igwilo, Ihuoma; Lurie, Ira S.

doi:10.1039/c5ay02206a

Cited by 12 publications

(21 citation statements)

References 27 publications

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“…However, HILIC is not widely used in human exposure analysis. A PFP column that exhibits hybrid stationary phase characteristics was used in a mixed-mode chromatography method and operated under both RPLC and HILIC conditions for SAMHSA-5 illicit drugs panel in urine (Clyde et al, 2015). Strategically combining RPLC, HILIC and PFP columns can capture and elute a wide range of both polar and hydrophilic metabolites in human matrices, covering a wider spectrum of the environmental organics chemical space (Tang et al, 2014, Alvarez-Segura et al, 2016, Shi et al, 2015).…”

Section: Advances In Analytical Tools For Profiling the Environmenmentioning

confidence: 99%

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Andra

Austin

Patel

et al. 2017

Environment International

124

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Global profiling of xenobiotics in human matrices in an untargeted mode is gaining attention for studying the environmental chemical space of the human exposome. Defined as the study of a comprehensive inclusion of environmental influences and associated biological responses, human exposome science is currently evolving out of the metabolomics science. In analogy to the latter, the development and applications of high resolution mass spectrometry (HRMS) has shown potential and promise to greatly expand our ability to capture the broad spectrum of environmental chemicals in exposome studies. HRMS can perform both untargeted and targeted analysis because of its capability of full- and/or tandem-mass spectrum acquisition at high mass accuracy with good sensitivity. The collected data from target, suspect and non-target screening can be used not only for the identification of environmental chemical contaminants in human matrices prospectively but also retrospectively. This review covers recent trends and advances in this field. We focus on advances and applications of HRMS in human biomonitoring studies, and data acquisition and mining. The acquired insights provide stepping stones to improve understanding of the human exposome by applying HRMS, and the challenges and prospects for future research.

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Section: Advances In Analytical Tools For Profiling the Environmenmentioning

confidence: 99%

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Andra

Austin

Patel

et al. 2017

Environment International

124

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“…Reproduced from Clyde, C., Blake, S., Obrien, S., Igwilo, I., Lurie, I. S., Application of mixed‐mode ultra high performance liquid chromatography to forensic drug analysis, Anal. Methods , 2015, 7 , 9763–9772 .…”

Section: Applications Of Liquid Separation Ms To the Analysis Of Illimentioning

confidence: 99%

Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Moini

2018

Electrophoresis

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In the past few years, there has been a significant effort by the forensic science community to develop new scientific techniques for the analysis of forensic evidence. Forensic chemists have been spearheaded to develop information-rich confirmatory technologies and techniques and apply them to a broad array of forensic challenges. The purpose of these confirmatory techniques is to provide alternatives to presumptive techniques that rely on data such as color changes, pattern matching, or retention time alone, which are prone to more false positives. To this end, the application of separation techniques in conjunction with mass spectrometry has played an important role in the analysis of forensic evidence. Moreover, in the past few years the role of liquid separation techniques, such as liquid chromatography and capillary electrophoresis in conjunction with mass spectrometry, has gained significant tractions and have been applied to a wide range of chemicals, from small molecules such as drugs and explosives, to large molecules such as proteins. For example, proteomics and peptidomics have been used for identification of humans, organs, and bodily fluids. A wide range of HPLC techniques including reversed phase, hydrophilic interaction, mixed-mode, supercritical fluid, multidimensional chromatography, and nanoLC, as well as several modes of capillary electrophoresis mass spectrometry, including capillary zone electrophoresis, partial filling, full filling, and micellar electrokenetic chromatography have been applied to the analysis drugs, explosives, and questioned documents. In this article, we review recent (2015-2017) applications of liquid separation in conjunction with mass spectrometry to the analysis of forensic evidence.

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“…Therefore, employing a second separation technique in which the separation results are orthogonal to the initial procedure is highly desirable. TLC [7], UHPLC [8][9][10][11], GC [7,12,13], MEKC [14], and ultra high performance supercritical fluid chromatography (UHPSFC) [15,16] have all been employed for the achiral separation of synthetic cathinones, while HPLC [17][18][19], GC [17], CE [20][21][22], CEC [19], and SFC [19] have been utilized for the separation of chiral synthetic cathinones. These studies have been limited in the number of controlled substances and/or positional isomers separated.…”

Section: Introductionmentioning

confidence: 99%

Comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones

Carnes

O’Brien

Szewczak

et al. 2017

J of Separation Science

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A comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones has been conducted. Nine different mixtures of bath salts were analyzed in this study. The three different chromatographic techniques were examined using a general set of controlled synthetic cathinones as well as a variety of other synthetic cathinones that exist as positional isomers. Overall 35 different synthetic cathinones were analyzed. A variety of column types and chromatographic modes were examined for developing each separation. For the ultra high performance supercritical fluid chromatography separations, analyses were performed using a series of Torus and Trefoil columns with either ammonium formate or ammonium hydroxide as additives, and methanol, ethanol or isopropanol organic solvents as modifiers. Ultra high performance liquid chromatographic separations were performed in both reversed phase and hydrophilic interaction chromatographic modes using SPP C18 and SPP HILIC columns. Gas chromatography separations were performed using an Elite-5MS capillary column. The orthogonality of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography was examined using principal component analysis. For the best overall separation of synthetic cathinones, the use of ultra high performance supercritical fluid chromatography in combination with gas chromatography is recommended.

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Application of mixed-mode ultra high performance liquid chromatography to forensic drug analysis

Abstract: Methodology is presented for the rapid analysis of drugs of abuse using a single UHPLC column with orthogonal separation methods using different combinations of the same solutions in the solvent reservoir.

Cited by 12 publications

References 27 publications

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Trends in the application of high-resolution mass spectrometry for human biomonitoring: An analytical primer to studying the environmental chemical space of the human exposome

Applications of liquid‐based separation in conjunction with mass spectrometry to the analysis of forensic evidence

Comparison of ultra high performance supercritical fluid chromatography, ultra high performance liquid chromatography, and gas chromatography for the separation of synthetic cathinones

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