Application of Microorganisms to the Processing and Upgrading of Crude Oil and Fractions

Morales, M.; Ayala, Marcela; Vázquez-Duhalt, Rafael; Borgne, Sylvie Le

doi:10.1007/978-3-540-77587-4_205

Cited by 7 publications

(7 citation statements)

References 80 publications

(82 reference statements)

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“…The increasing global human population accompanied by extensive fossil energy consumption has posed serious threats to the environment and human health (Maass et al, 2015 ). Organosulfur (thiophenes) compounds found in crude oil and diesel are of particular concern due to their hazardous impact on human health and the ecosystem (Kilbane, 2006 ; Morales et al, 2010 ). Moreover, the sulfur oxide gases resulting from fuel combustion are a major cause of acid rain.…”

Section: Introductionmentioning

confidence: 99%

Biocatalytic Desulfurization Capabilities of a Mixed Culture during Non-Destructive Utilization of Recalcitrant Organosulfur Compounds

et al. 2016

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We investigated the biodesulfurization potential of a mixed culture AK6 enriched from petroleum hydrocarbons-polluted soil with dibenzothiophene (DBT) as a sulfur source. In addition to DBT, AK6 utilized the following compounds as sulfur sources: 4-methyldibenzothiophene (4-MDBT), benzothiophene (BT), and 4,6- dimethyldibenzothiophene (4,6-DM-DBT). None of these compounds supported the growth of AK6 as the sole carbon and sulfur source. AK6 could not grow on dibenzylsulfide (DBS) as a sulfur source. The AK6 community structure changed according to the provided sulfur source. The major DGGE bands represented members of the genera Sphingobacterium, Klebsiella, Pseudomonas, Stenotrophomonas, Arthrobacter, Mycobacterium, and Rhodococcus. Sphingobacterium sp. and Pseudomonas sp. were abundant across all cultures utilizing any of the tested thiophenic S-compounds. Mycobacterium/Rhodococcus spp. were restricted to the 4-MDBT culture. The 4-MDBT culture had the highest species richness and diversity. Biodesulfurization of DBT by resting cells of AK6 produced 2-hydroxybiphenyl (2-HBP) in addition to trace amounts of phenylacetate. AK6 transformed DBT to 2-hydroxybiphenyl with a specific activity of 9 ± 0.6 μM 2-HBP g dry cell weight−1 h−1. PCR confirmed the presence in the AK6 community of the sulfur-specific (4S) pathway genes dszB and dszC. Mixed cultures hold a better potential than axenic ones for the development of a biodesulfurization technology.

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Section: Introductionmentioning

confidence: 99%

Biocatalytic Desulfurization Capabilities of a Mixed Culture during Non-Destructive Utilization of Recalcitrant Organosulfur Compounds

et al. 2016

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“…The increasing global human population and extensive fossil energy consumption have caused serious threats to the environment and human health [33]. Organosulfur [thiophenes] compounds found in crude oil and diesel have particular importance due to their hazardous impact on the ecosystem and human health [34]. Most studies conducted on microbial desulfurization have adopted axenic cultures of selected microorganisms.…”

Section: Biodesulfurization Capabilities Of a Mixed Culturementioning

confidence: 99%

The Role of Microorganisms and Productions in Biodesulfurization of Fossiel Fuels

Raheb¹

2016

J Microb Biochem Technol

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“…The use of microorganisms to improve the refining of problematic petroleum feedstocks has been proposed [1]. The main target has been the precombustion removal of sulfur (desulfurization) in diesel oils in a process called biodesulfurization (BDS) [2].…”

Section: Introductionmentioning

confidence: 99%

“…Precombustion removal of sulfur-containing compounds is important in order to limit SOx emissions into the atmosphere during the combustion of petroleum-derived fuels and therefore avoid the formation of acid rain. Stringent restrictions on the levels of sulfur in transportation fuels now exist in most countries [1]. Dibenzothiophene (DBT) and DBT with alkyl substituents in the 4-and 6-position flanking the sulfur atom ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Protocols for the Isolation and Preliminary Characterization of Bacteria for Biodesulfurization and Biodenitrogenation of Petroleum-Derived Fuels

Morales

Borgne

2014

Springer Protocols Handbooks

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The use of microorganisms to improve fuel quality has been proposed, and in this chapter we describe basic experimental procedures for the isolation and characterization of bacteria for biodesulfurization and biodenitrogenation with dibenzothiophene and carbazole as model compounds for each application, respectively. The presented protocols should be considered as a starting point and adapted to other problematic compounds and conditions. Basic protocols for the evaluation of the treatment of real oil fractions are also presented.

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Application of Microorganisms to the Processing and Upgrading of Crude Oil and Fractions

Cited by 7 publications

References 80 publications

Biocatalytic Desulfurization Capabilities of a Mixed Culture during Non-Destructive Utilization of Recalcitrant Organosulfur Compounds

Biocatalytic Desulfurization Capabilities of a Mixed Culture during Non-Destructive Utilization of Recalcitrant Organosulfur Compounds

The Role of Microorganisms and Productions in Biodesulfurization of Fossiel Fuels

Protocols for the Isolation and Preliminary Characterization of Bacteria for Biodesulfurization and Biodenitrogenation of Petroleum-Derived Fuels

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