Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang)

Ren, Yong; Zhang, Ping; Yan, Dan; Yan, Yan; Chen, Longhu; Qiu, Lingling; Mei, Zhinan; Xiao, Xiaohe

doi:10.1016/j.apsb.2012.02.013

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…IMC can thus be applied as a real-time detector for a variety of microbial contaminants because all living microorganisms dissipate parts of the Gibbs energy of the substrates they assimilated in the form of heat (Gram and Sogaard, 1985; Wadsö, 1995; Von Stockar and Liu, 1999; Trampuz et al, 2007a; Maskow et al, 2012). The application of IMC for the quantification of microbial contamination by various species is already described in the literature (Levin, 1977; Xie et al, 1995; Trampuz et al, 2007a, b; Braissant et al, 2010b; Bonkat et al, 2011; Ren et al, 2012; Gysin et al, 2018). Interestingly, almost exclusively liquid cultures were used for the quantification of the different bacteria in samples from different origins.…”

Section: Introductionmentioning

confidence: 99%

Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique

2019

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Modern isothermal microcalorimeters (IMC) are able to detect the metabolic heat of bacteria with an accuracy sufficient to recognize even the smallest traces of bacterial contamination of water, food, and medical samples. The modern IMC techniques are often superior to conventional detection methods in terms of the detection time, reliability, labor, and technical effort. What is missing is a systematic analysis of the influence of the cultivation conditions on calorimetric detection. For the acceptance of IMC techniques, it is advantageous if already standardized cultivation techniques can be combined with calorimetry. Here we performed such a systematic analysis using Lactobacillus plantarum as a model bacterium. Independent of the cultivation techniques, IMC detections were much faster for high bacterial concentrations (>102 CFU⋅mL–1) than visual detections. At low bacterial concentrations (<102 CFU⋅mL–1), detection times were approximately the same. Our data demonstrate that all kinds of traditional cultivation techniques like growth on agar (GOA) or in agar (GIA), in liquid media (GL) or on agar after enrichment via membrane filtration (GF) can be combined with IMC. The order of the detection times was GF < GIA ≈ GL ≈ GOA. The observed linear relationship between the calorimetric detection times and the initial bacterial concentrations can be used to quantify the bacterial contamination. Further investigations regarding the correlation between the filling level (in mm) of the calorimetric vessel and the specific maximum heat flow (in μW⋅g–1) illustrated two completely different results for liquid and solid media. Due to the better availability of substrates and the homogeneous distribution of bacteria growing in a liquid medium, the volume-related maximum heat flow was independent on the filling level of the calorimetric vessels. However, in a solid medium, the volume-related maximum heat flow approached a threshold and achieved a maximum at low filling levels. This fundamentally different behavior can be explained by the spatial limitation of the growth of bacterial colonies and the reduced substrate supply due to diffusion.

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Section: Introductionmentioning

confidence: 99%

Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique

2019

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“…NS normal samples; TS high-temperature accelerated samples; LS extreme illumination accelerated samples; ES samples exposed to the environment injections would still remain unexplained. Many laboratory studies have demonstrated that QC relying mainly on is insufficient for herbal products since it cannot reflect the clinical efficacy and any unknown materials may pose a risk to patients [18,31].…”

Section: Results Of Statistical Analysismentioning

confidence: 99%

Quality fluctuation detection of an herbal injection based on biological fingerprint combined with chemical fingerprint

et al. 2014

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Herbal injection is one of the most important preparations of traditional Chinese medicine. More than 130 types of herbal injections are used clinically for 400 million patients annually with total sales of over four billion US dollars per year. However, the current quality control (QC) methods relying mainly on chemical fingerprints (CF) can hardly ensure quality and safety of the herbal injections with complex chemical composition and have resulted in an increase in serious adverse drug reactions. In this study, a comprehensive approach for the QC of a controversial herbal injection Shuang-Huang-Lian lyophilized powder (SHL) was established based on the quality fluctuation detection by a combination of CF and biological fingerprint (BF). High-performance liquid chromatography and the impedance-based xCELLigence system were applied to establish the CF and BF, respectively. In addition, multivariate analysis was performed to evaluate the discriminant ability of the two methods. The results showed that being subjected to environmental influence like oxygen/air, high temperature, and extreme illumination could lead to quality fluctuation of SHL. The combination of chemical and biological fingerprint method is a more powerful tool for the QC of SHL because it can clearly discriminate different groups of abnormal samples. This method can be used for the detection of quality fluctuation of SHL and can provide reference for the quality control of other herbal injections.

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“…The microcalorimetric test was conducted on a 3114/3236 thermal active monitor (TAM TM ) air eight-channel isothermal calorimeter manufactured by Thermometric AB, Stochholm, Sweden. The structure and work principle of this instrument was detailed in other literatures (Ren et al, 2012) or web site of the manufacturer. The measurement was conducted as previously reported (Zheng et al, 2007).…”

Section: Microcalorimetric Measurementsmentioning

confidence: 99%

Microcalorimetric study of microbial activity changes of acrisol in subtropical China under three different land management

ShiChun¹,

Linlin²,

Jiang³

et al. 2014

Afr. J. Agric. Res.

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The microcalorimetric method was applied to analyze the influences of successive reforestations with Eucalyptus granddis × E. uophylla, Pinus massoniana, and continuous sugarcane production on the soil microbial activities. 500 g of each refined representative sample was collected in rainy and dry seasons from four 100 m 2 homogeneously and perfectly defined acrisol quadrats in subtropical China. Two of them were Eucalyptus plantations with 10 (E10) and 20 (E20) year-old stands. The other two were 10-year-old pine tree plantation (P10) and sugarcane land (SL10) correspondingly contiguous to E10 and E20 and used as references for E10 and E20, respectively. Blocks of E10/P10 were 1500 m away from that of E20/SL10. Microcalorimetric experiments were carried out using 1.2 g soil samples and 0.6 ml of solution containing 5.0 mg of glucose and 5.0 mg of ammonium sulphate at 28°C. The effects of land management practice on soil quality were examined by measuring their physicochemical and biological properties. The results showed that: 1) zymogeneous bacteria were the dominated microbes in the land of continuous sugarcane production, but autochthonous floras were the ones for forest; 2) when compared with its control (SL10), land of eucalyptus (E20) had lower soil packing degree and inhibitory effect on microbial activities, but higher seasonal fluctuation in microbe constitution and activities under the same circumstance; 3) compared with its control P10, the land of eucalyptus E10 had alike soil packing degree and higher seasonal fluctuation degree of microbial activities, but lower inhabiting degree of soil microbial metabolism.

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Application of microcalorimetry of Escherichia coli growth and discriminant analysis to the quality assessment of a Chinese herbal injection (Yinzhihuang)

Cited by 11 publications

References 21 publications

Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique

Rapid Calorimetric Detection of Bacterial Contamination: Influence of the Cultivation Technique

Quality fluctuation detection of an herbal injection based on biological fingerprint combined with chemical fingerprint

Microcalorimetric study of microbial activity changes of acrisol in subtropical China under three different land management

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