Application of Microalgae Consortia/Cocultures in Wastewater Treatment

Santos, Vinícius Zotti dos; Vieira, Karem Rodrigues; Nass, Pricila Pinheiro; Zepka, Leila Queiroz; Jacob‐Lopes, Eduardo

doi:10.1007/978-981-16-0518-5_5

Cited by 6 publications

(3 citation statements)

References 121 publications

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“…mutabilis following amphotericin B treatment, these results reveal several combinatorial interactions between the bacteria and E. mutabilis, the bacteria and the fungus, and the fungus and E. mutabilis. Consistent with synthetic co-culture work [57,58,59, 60] these results indicate that the fungus and bacteria assist in the resistance of E. mutabilis to Cd. Further, they reveal multiple organismal interactions in uence the response of the co-culture to HM challenge.…”

Section: Discussionsupporting

confidence: 87%

Mutually beneficial FAB consortia fortify stress resistance of Euglena mutabilis: evidence from sequencing, antibiotics, and Cd challenges

Kaszecki,

Palberg,

Grant

et al. 2023

Preprint

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Background Synthetic algal-fungal and algal-bacterial cultures have been investigated for technological applications because the microbe interactions enhance growth and improve stress tolerance of the co-cultures. Yet these studies often disregarded natural consortia due to the complexity of environmental samples. The protist Euglena mutabilis is found in association with other microbes in acidic environments with high heavy metal (HM) concentrations. This may suggest that microbial interactions are essential for the alga’s ability to tolerate these extreme environments. Our study assessed the Cd tolerance of a natural fungal-algal-bacterial (FAB) association where the algae is replaced by the photosynthetic protist E. mutabilis. Results This study provides the first assessment of antimycotic and antibiotic agents on E. mutabilis. Our results indicate that suppression of associated fungal and bacterial partners significantly decreases the number of viable E. mutabilis cells upon Cd exposure. However, axenic Euglena gracilis recovered and grew well following antibiotic treatments. Interestingly, both Euglena species displayed increased chlorophyll production upon Cd exposure. Finally, the constituent organisms in the E. mutabilis FAB consortia were identified using PacBio sequencing to be a Talaromyces sp and Acidiphilium acidophilum. Conclusion This study uncovers a possible tripartite symbiotic relationship, a FAB consortia, that withstands exposure to high concentrations of HM. This unique fungus, bacterium, and E. mutabilis interaction strengthens the photobiont’s resistance to Cd and provides a model for the types of FAB interactions that could be used to create a self-sustaining bioremediation technology.

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Section: Discussionsupporting

confidence: 87%

Mutually beneficial FAB consortia fortify stress resistance of Euglena mutabilis: evidence from sequencing, antibiotics, and Cd challenges

Kaszecki,

Palberg,

Grant

et al. 2023

Preprint

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“…[157,158] The commercial application of microalgae for biomass production using wastewater has been started since 1950s. [159] Wastewater as a growth medium for microalgae that cannot only utilize inorganic and organic nutrients metabolically but also in the cooperation with microbes to degrade/metabolize other pollutants with significant public health and environmental threat, like polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), pesticides, insecticides, phenolics, petroleum hydrocarbons (PHCs), heavy metals, and antibiotics, [160][161][162] is a readily accessible and inexpensive approach [163,164] for WWT coupled with microalgae growth to offer cost-effective biomass feedstock and biofuel generation. [165][166][167] Initially, research into the use of wastewater-grown microalgae for energy production started with the use of microalgae as a feedstock for methane production.…”

Section: Potential Development Of Genetic Engineering Of Microalgae F...mentioning

confidence: 99%

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Kuo

Yang

Chien

et al. 2022

Biotechnology Journal

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Microalgae, a group of photosynthetic microorganisms rich in diverse and novel bioactive metabolites, have been explored for the production of biofuels, high value-added compounds as food and feeds, and pharmaceutical chemicals as agents with therapeutic benefits. This article reviews the development of omics resources and genetic engineering techniques including gene transformation methodologies, mutagenesis, and genome-editing tools in microalgae biorefinery and wastewater treatment (WWT).The introduction of these enlisted techniques has simplified the understanding of complex metabolic pathways undergoing microalgal cells. The multiomics approach of the integrated omics datasets, big data analysis, and machine learning for the discovery of objective traits and genes responsible for metabolic pathways was reviewed. Recent advances and limitations of multiomics analysis and genetic bioengineering technology to facilitate the improvement of microalgae as the dual role of WWT and biorefinery feedstock production are discussed.

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“…Microalgae may produce a wide range of high-value bioactive chemicals, including carbohydrates, proteins, lipids, essential fatty acids, pigments, vitamins and antioxidants, among other thing (Udayan et al 2021). Microalgae are being regarded as prospective bioremediators of urban and agro-industrial effluents, allowing the integration of bio refineries, the utilization of gaseous effluents and the development of added value biomass in this context (dos Santos et al 2021). Concern about looking young and beautiful has grown in the previous decade, prompting many customers to use anti-aging cosmetics on a daily basis.…”

Section: Introductionmentioning

confidence: 99%

Transformation and Expression of Gene in Microalgae: From Tools to Applications

Usmaan¹,

Rao²

2021

Agrobiol. Records

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Microalgae provides an excellent platform for foreign genes expression. Chloroplast and Nucleus based transformation of transgenes in algae is possible after construct designing (including different markers and promoters) and codon optimization. For better recombination, different and effective promoters and reporter genes can be selected. Chloroplast mediated transformation is much more effective than nuclear based transformation for transgene analysis owing to higher copy number of chloroplast in unit area. Microalgae based products are excellent target for production of wide variety of bio products such as vaccines, antibiotics and drugs against different diseases. These alternative approaches facilitate enhanced biodiesel production through fast growth of microalgae in heterotrophic environment. Also, low risks are attributed owing to their nonhuman consumption property. Biosafety and risk assessment analysis should be carried out before releasing of genetically modified microalgae in the environment considering research ethical code. Microalgae provide cheap, efficient and high yielding alternatives to plants. The review emphasizes microalgae based expression approach with key steps involved in detail.

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Application of Microalgae Consortia/Cocultures in Wastewater Treatment

Cited by 6 publications

References 121 publications

Mutually beneficial FAB consortia fortify stress resistance of Euglena mutabilis: evidence from sequencing, antibiotics, and Cd challenges

Mutually beneficial FAB consortia fortify stress resistance of Euglena mutabilis: evidence from sequencing, antibiotics, and Cd challenges

Multiomics approaches and genetic engineering of metabolism for improved biorefinery and wastewater treatment in microalgae

Transformation and Expression of Gene in Microalgae: From Tools to Applications

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