Application of Micro-CT in Soft Tissue Specimen Imaging

Roque‐Torres, Gina Delia

doi:10.1007/978-3-030-16641-0_10

Cited by 2 publications

(2 citation statements)

References 189 publications

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“…Since the first reports on CECT about 15 years ago 48,49 , several CESAs have been developed and established 50 . Among the most wellknown CESAs are inorganic iodine solutions such as Lugol's iodine (I 2 KI), Osmium tetroxide (OsO 4 ), Ioxaglate (Hexabrix), CA4+, and certain polyoxometalates (POMs) such as Phosphotungstic acid (PTA) and Phosphomolybdic acid (PMA) 15,51 . Despite the recent breakthroughs in the field of CECT, the nondestructive visualization of several soft tissues and organs, without inducing tissue shrinkage and deformation, remains challenging 31,52 .…”

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confidence: 99%

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Maes

Pestiaux

Marino³

et al. 2022

Nat Commun

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Biological tissues comprise a spatially complex structure, composition and organization at the microscale, named the microstructure. Given the close structure-function relationships in tissues, structural characterization is essential to fully understand the functioning of healthy and pathological tissues, as well as the impact of possible treatments. Here, we present a nondestructive imaging approach to perform quantitative 3D histo(patho)logy of biological tissues, termed Cryogenic Contrast-Enhanced MicroCT (cryo-CECT). By combining sample staining, using an X-ray contrast-enhancing staining agent, with freezing the sample at the optimal freezing rate, cryo-CECT enables 3D visualization and structural analysis of individual tissue constituents, such as muscle and collagen fibers. We applied cryo-CECT on murine hearts subjected to pressure overload following transverse aortic constriction surgery. Cryo-CECT allowed to analyze, in an unprecedented manner, the orientation and diameter of the individual muscle fibers in the entire heart, as well as the 3D localization of fibrotic regions within the myocardial layers. We foresee further applications of cryo-CECT in the optimization of tissue/food preservation and donor banking, showing that cryo-CECT also has clinical and industrial potential.

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mentioning

confidence: 99%

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Maes

Pestiaux

Marino³

et al. 2022

Nat Commun

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“…Despite the considerable advances over the last years (i.e: higher image resolution, more efficient reconstruction algorithms etc), the main limitation of micro-CT remains the high amount of radiation dose that it would transmit to the patient. Even though a trade-off between the radiation dose and the desired image quality can be made, still it is at unacceptable levels as it may induce cancer to the patient [52,53]. The exponentially growing number of studies focusing on micro-CT scanners, underpins the increased importance of this imaging modality and the ongoing optimization efforts to provide in-vivo high resolution scanning [11,51,52].…”

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confidence: 99%

Improved automated early detection of breast cancer based on high resolution 3D micro-CT microcalcification images

et al. 2022

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Background The detection of suspicious microcalcifications on mammography represents one of the earliest signs of a malignant breast tumor. Assessing microcalcifications’ characteristics based on their appearance on 2D breast imaging modalities is in many cases challenging for radiologists. The aims of this study were to: (a) analyse the association of shape and texture properties of breast microcalcifications (extracted by scanning breast tissue with a high resolution 3D scanner) with malignancy, (b) evaluate microcalcifications’ potential to diagnose benign/malignant patients. Methods Biopsy samples of 94 female patients with suspicious microcalcifications detected during a mammography, were scanned using a micro-CT scanner at a resolution of 9 μm. Several preprocessing techniques were applied on 3504 extracted microcalcifications. A high amount of radiomic features were extracted in an attempt to capture differences among microcalcifications occurring in benign and malignant lesions. Machine learning algorithms were used to diagnose: (a) individual microcalcifications, (b) samples. For the samples, several methodologies to combine individual microcalcification results into sample results were evaluated. Results We could classify individual microcalcifications with 77.32% accuracy, 61.15% sensitivity and 89.76% specificity. At the sample level diagnosis, we achieved an accuracy of 84.04%, sensitivity of 86.27% and specificity of 81.39%. Conclusions By studying microcalcifications’ characteristics at a level of details beyond what is currently possible by using conventional breast imaging modalities, our classification results demonstrated a strong association between breast microcalcifications and malignancies. Microcalcification’s texture features extracted in transform domains, have higher discriminating power to classify benign/malignant individual microcalcifications and samples compared to pure shape-features.

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Application of Micro-CT in Soft Tissue Specimen Imaging

Cited by 2 publications

References 189 publications

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

Improved automated early detection of breast cancer based on high resolution 3D micro-CT microcalcification images

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