Application of Measurement Method for Lactic Acid Bacteria Content
on Coccus Including Yoghurt

Oh, Yun; Lee, Mi Seon; Ahn, Yujin; Ahn, Jang‐Hyuk

doi:10.35732/ctlabp.2015.3.1.34

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Maintaining viable cell counts of lactic acid bacteria is essential for the probiotic pelleting process [ 45 ]. Thus, it is necessary to select strains with high resistance to heat.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis and Physiological Properties of Limosilactobacillus fermentum SMFM2017-NK2 with Ability to Inflammatory Bowel Disease

2023

View full text Add to dashboard Cite

The objective of this study was to evaluate the anti-inflammatory effect of Latilactobacillus sakei SMFM2017-NK1 (LS1), L. sakei SMFM2017-NK3 (LS2), and Limosilactobacillus fermentum SMFM2017-NK2 (LF) on colitis using an animal model. DSS (dextran sulfate sodium salt) was orally injected into C57BL/6N mice to induce inflammation in the colon for seven days. Colitis mice models were treated with LS1, LS2, and LF, respectively, and Lacticaseibacillus rhamnosus GG (LGG) was used as a positive control. During oral administration of lactic acid bacteria, the weights of the mice were measured, and the disease activity index (DAI) score was determined by judging the degree of diarrhea and bloody stool. When comparing the differences between the minimum weight after DSS administration and the maximum weight after lactic acid bacteriaadministration were compared, the LF-treated group showed the highest weight gain at 8.91%. The DAI scores of the LF, LS2, and LGG groups were lower than that of the control group. After sacrifice, mRNA expression levels for proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IFN-γ) and mediators (iNOS and COX-2) in the colon were measured. LF was selected as a superior strain for anti-inflammation in the colon. It was further analyzed to determine its biochemical characteristics, cytotoxicity, and thermal stability. Catalase and oxidase activities for LF were negative. In cytotoxicity and heat stability tests, the LF group had higher cell viability than the LGG group. The genome of LF was obtained, and 5682 CDS, 114 tRNA, 2 RNA, and 5 repeat regions were predicted. Especially, LF could be distinguished from the other three L. fermentum strains based on taxonomic profiling, specific orthologous genes of the strain, and genomic variants. The results of this study suggest that L. fermentum SMFM2017-NK2 is a novel strain with an anti-inflammatory effect on colitis.

show abstract

“…Maintaining viable cell counts of lactic acid bacteria is essential for the probiotic pelleting process [ 45 ]. Thus, it is necessary to select strains with high resistance to heat.…”

Section: Resultsmentioning

confidence: 99%

Comparative Genomic Analysis and Physiological Properties of Limosilactobacillus fermentum SMFM2017-NK2 with Ability to Inflammatory Bowel Disease

2023

View full text Add to dashboard Cite

show abstract

“…In addition, the case of products mixed with seven different species accounted for 24.7%, followed by nineteen (11.8%) and six (11.8%) LAB mixtures, with an average of nine LAB combined in the products [19]. In the Korea Food Code, the standard for the number of LAB in fermented milk products is more than 10 7 CFU/ml, while the level for condensed fermented milk products and probiotic products is more than 10 8 CFU/ml [28].…”

Section: Quantification Of Lab In Commercial Productsmentioning

confidence: 99%

“…The quantity of LAB was largest in BL agar, followed by PCA with BCP and MRS agars. In a similar study, Oh et al (2015) compared culture media (BCP plate count agar, PCA with BCP, and MRS) to quantify LAB in yogurt, and the number of colonies was highest on BCP plate count agar, followed by MRS and PCA with BCP agars [28]. One probiotic product (P11) contained B. bifidum, a species that did not grow well on PCA with BCP medium.…”

Section: Quantification Of Lab In Commercial Productsmentioning

confidence: 99%

Comparative Evaluation of Culture Media for Quantification of Lactic Acid Bacteria in Various Dairy Products

Kim

Lee

Gwak

et al. 2023

Microbiol. Biotechnol. Lett.

View full text Add to dashboard Cite

Dairy products are extensively used as carriers of probiotic strains that have potential health benefits. Assessment of the viability of probiotic strains during manufacturing is important to ensure that products meet recommended levels. Hence, the method for accurately quantifying lactic acid bacteria (LAB) in probiotic or dairy products is required. The present study aims to examine the performance of de-Man Rogosa Sharpe (MRS), plate count agar with bromocresol purple (PCA with BCP), and glucose blood liver (BL) agars recommended in the Korea Food Code guidelines for counting LAB. Analysis of the performance of culture media containing 19 lactic acid bacterial species commonly encountered in probiotic and dairy products showed no statistically significant difference between 18 reference strains and three culture media (p > 0.01). Furthermore, the suitability of three culture media was verified for the quantitative assessment of LAB in 25 probiotic and dairy products. The number of LAB in three culture media was determined to be more than 10 7 colony-forming unit (CFU)/ml for fermented milk products and 10 8 CFU/ml for condensed fermented milk and probiotic products, indicating that they all satisfied the Korea Food Code guidelines. Moreover, there was no statistically significant difference in the amount of LAB counted in all three culture media, suggesting that they can be used to isolate or enumerate LAB in commercial products. Finally, three culture media will be useful for isolating and enumerating LAB from fermented foods as well as gut microflora.

show abstract

Application of Measurement Method for Lactic Acid Bacteria Content on Coccus Including Yoghurt

Cited by 2 publications

References 0 publications

Comparative Genomic Analysis and Physiological Properties of Limosilactobacillus fermentum SMFM2017-NK2 with Ability to Inflammatory Bowel Disease

Comparative Genomic Analysis and Physiological Properties of Limosilactobacillus fermentum SMFM2017-NK2 with Ability to Inflammatory Bowel Disease

Comparative Evaluation of Culture Media for Quantification of Lactic Acid Bacteria in Various Dairy Products

Contact Info

Product

Resources

About