Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging in Combination with LC–MS in Pharmacokinetic Study of Metformin

Strnad, Štěpán; Vrkoslav, Vladimír; Klimšová, Zuzana; Zemenová, Jana; Cvačka, Josef; Maletı́nská, Lenka; Sýkora, David

doi:10.4155/bio-2017-0190

Cited by 4 publications

(4 citation statements)

References 31 publications

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“…Preparation of liver samples for mass spectrometry imaging (MSI) analysis and 2,5-dihydroxybenzoic acid (DHB) sublimation was performed according to our previously published method [ 31 ]. For triacylglyceride analysis, the NaDHB solution (10 mg mL −1 in acetone) was sprayed using an iMatrixSpray automatic sprayer (Tardo Gmbh, Subingen, Switzerland) with typical spray parameters for dry spraying [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Lipidized PrRP Analog Exhibits Strong Anti-Obesity and Antidiabetic Properties in Old WKY Rats with Obesity and Glucose Intolerance

et al. 2023

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Prolactin-releasing peptide (PrRP) is an anorexigenic neuropeptide that has potential for the treatment of obesity and its complications. Recently, we designed a palmitoylated PrRP31 analog (palm11-PrRP31) that is more stable than the natural peptide and able to act centrally after peripheral administration. This analog acted as an anti-obesity and glucose-lowering agent, attenuating lipogenesis in rats and mice with high-fat (HF) diet-induced obesity. In Wistar Kyoto (WKY) rats fed a HF diet for 52 weeks, we explored glucose intolerance, but also prediabetes, liver steatosis and insulin resistance-related changes, as well as neuroinflammation in the brain. A potential beneficial effect of 6 weeks of treatment with palm11-PrRP31 and liraglutide as comparator was investigated. Liver lipid profiles, as well as urinary and plasma metabolomic profiles, were measured by lipidomics and metabolomics, respectively. Old obese WKY rats showed robust glucose intolerance that was attenuated by palm11-PrRP31, but not by liraglutide treatment. On the contrary, liraglutide had a beneficial effect on insulin resistance parameters. Despite obesity and prediabetes, WKY rats did not develop steatosis owing to HF diet feeding, even though liver lipogenesis was enhanced. Plasma triglycerides and cholesterol were not increased by HFD feeding, which points to unincreased lipid transport from the liver. The liver lipid profile was significantly altered by a HF diet that remained unaffected by palm11-PrRP31 or liraglutide treatment. The HF-diet-fed WKY rats revealed astrogliosis in the brain cortex and hippocampus, which was attenuated by treatment. In conclusion, this study suggested multiple beneficial anti-obesity-related effects of palm11-PrRP31 and liraglutide in both the periphery and brain.

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Section: Methodsmentioning

confidence: 99%

Lipidized PrRP Analog Exhibits Strong Anti-Obesity and Antidiabetic Properties in Old WKY Rats with Obesity and Glucose Intolerance

et al. 2023

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“…Preparation of brain free-floating sections (n = 3) for mass spectrometry imaging (MSI) analysis and 1,5-diaminonaphthalene (DAN) matrix spraying was performed according to our previously published method [28]. SCiLS Lab 2016b software (SCiLS GmbH, Germany) was used for statistical analysis as described previously [29].…”

Section: Brain Maldi Msimentioning

confidence: 99%

Feeding High-Fat Diet Accelerates Development of Peripheral and Central Insulin Resistance and Inflammation and Worsens AD-like Pathology in APP/PS1 Mice

Mengr,

Strnadová,

Strnad

et al. 2023

Nutrients

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Alzheimer’s disease (AD) is a progressive brain disorder characterized by extracellular amyloid-β (Aβ) plaques, intracellular neurofibrillary tangles formed by hyperphosphorylated Tau protein and neuroinflammation. Previous research has shown that obesity and type 2 diabetes mellitus, underlined by insulin resistance (IR), are risk factors for neurodegenerative disorders. In this study, obesity-induced peripheral and central IR and inflammation were studied in relation to AD-like pathology in the brains and periphery of APP/PS1 mice, a model of Aβ pathology, fed a high-fat diet (HFD). APP/PS1 mice and their wild-type controls fed either a standard diet or HFD were characterized at the ages of 3, 6 and 10 months by metabolic parameters related to obesity via mass spectroscopy, nuclear magnetic resonance, immunoblotting and immunohistochemistry to quantify how obesity affected AD pathology. The HFD induced substantial peripheral IR leading to central IR. APP/PS1-fed HFD mice had more pronounced IR, glucose intolerance and liver steatosis than their WT controls. The HFD worsened Aβ pathology in the hippocampi of APP/PS1 mice and significantly supported both peripheral and central inflammation. This study reveals a deleterious effect of obesity-related mild peripheral inflammation and prediabetes on the development of Aβ and Tau pathology and neuroinflammation in APP/PS1 mice.

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“…Laser ablation is a powerful and promising technique [67] for a wide range of applications, from biomedicine (tissue excision) [68], to characterization of biomaterials in analytical chemistry (assisted-matrix laser desorption ionization, MALDI) [69,70] and the structuring of polymers in the field of microelectronics [67], in addition to the preservation of works of art [71].…”

Section: Matrix-assisted Pulsed Laser Evaporation (Maple) and C-maplementioning

confidence: 99%

“…The films of FN and VN have been shown to promote the efficient adhesion of cells to substrates [70].…”

mentioning

confidence: 99%

Coatings Functionalization via Laser versus Other Deposition Techniques for Medical Applications: A Comparative Review

et al. 2022

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The development of new biological devices in response to market demands requires continuous efforts for the improvement of products’ functionalization based upon expansion of the materials used and their fabrication techniques. One viable solution consists of a functionalization substrate covered by layers via an appropriate deposition technique. Laser techniques ensure an enhanced coating’s adherence to the substrate and improved biological characteristics, not compromising the mechanical properties of the functionalized medical device. This is a review of the main laser techniques involved. We mainly refer to pulse laser deposition, matrix-assisted, and laser simple and double writing versus some other well-known deposition methods as magnetron sputtering, 3D bioprinting, inkjet printing, extrusion, solenoid, fuse-deposition modeling, plasma spray (PS), and dip coating. All these techniques can be extended to functionalize surface fabrication to change local morphology, chemistry, and crystal structure, which affect the biomaterial behavior following the chosen application. Surface functionalization laser techniques are strictly controlled within a confined area to deliver a large amount of energy concisely. The laser deposit performances are presented compared to reported data obtained by other techniques.

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Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging in Combination with LC–MS in Pharmacokinetic Study of Metformin

Cited by 4 publications

References 31 publications

Lipidized PrRP Analog Exhibits Strong Anti-Obesity and Antidiabetic Properties in Old WKY Rats with Obesity and Glucose Intolerance

Lipidized PrRP Analog Exhibits Strong Anti-Obesity and Antidiabetic Properties in Old WKY Rats with Obesity and Glucose Intolerance

Feeding High-Fat Diet Accelerates Development of Peripheral and Central Insulin Resistance and Inflammation and Worsens AD-like Pathology in APP/PS1 Mice

Coatings Functionalization via Laser versus Other Deposition Techniques for Medical Applications: A Comparative Review

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