Application of mathematical models and microfluidics in the analysis of saliva mixing with antiseptic solutions

Abstract

SummaryBackground/Aim: Human saliva offers many advantages over blood-based biochemical assays, therefore, becomes the biological fluid of interest. Once antiseptic solutions react with saliva, both fluids undergo significant changes of their biophysical properties, consequently, those changes have an impact on their principal function.Material and Methods: In this study, saliva was collected and mixed with 0,1% chlorhexidine digluconate solution, fluoride mouthwash, zinc-hydroxyapatite solution and CPP-ACP paste. Microfluidic PVC/Green tape chips within the experimental setup were used to simulate solution mixing. The chip had 2 inlets and 1 outlet, and channel was designed in Y shape without any obstacles. The inlet channels were set at a 60° angle. The channel width was 600 µm and the diameter of inlets and outlet was 2 mm. For better visualization, blue food coloring was added to the saliva. The procedure was recorded with digital USB microscope camera and afterwards the percentage of mixing was obtained by MATLAB programming language.Results: Obtained results show incomplete mixing of all the solutions with saliva. The value of mixed liquid, when mixing 0,1% chlorhexidine digluconate solution with saliva was 51,11%. In case of medium concentration fluoride mouthwash, result was 84,37%. Zinc hydroxyapatite solution obtained result of 85,24%, and the fourth tested solution, CPP-ACP paste, 83,89%.Conclusions: Analyzed mouthwashes exhibit specific, non uniform behavior during mixing with saliva. Microfluidic setups could be efficiently used in simulating real clinical conditions in laboratory settings. Image processing mathematical models are applicable, accurate and useful in determination of the interaction of saliva with commonly used antiseptic solutions.

Design of Epidemic Prevention Medical Special Clothing Based on Mathematical Model Analysis

“…According to the research on the design of special antiepidemic medical clothing, this article evaluates the quality of antiepidemic clothing from the aspects of functionality, material properties, comfort, ease of handling, and economy [18]. At the beginning of the design of antiepidemic clothing, this article took into account the needs of epidemic protection and established a quality evaluation index system for the design of antiepidemic clothing according to various factors such as policy factors, economic factors, technical factors, management factors, and disposal of antiepidemic clothing design [19]. rough the above analysis of the influencing factors of the quality evaluation of epidemic prevention clothing, this article first establishes the first-level quality index set A of the epidemic prevention clothing quality evaluation and the second-level evaluation index set B under it as follows:…”