Application of marker‐assisted backcross to introgress Bph3, Bph14 and Bph15 into an elite indica rice variety for improving its resistance to brown planthopper

Hu, Wei; Xiao, Hanxiang; Hu, Kan; Jiang, Yijun; Yang, Zhangping

doi:10.1111/pbr.12364

Cited by 20 publications

(23 citation statements)

References 42 publications

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“…The results indicated that the severity scores of rice lines were RHT<BPh68S<Lianyou8676<Fuhui676< TN1 (Figure 1). RHT scored 0.4 in this study, exhibited a high resistance (HR) to BPH, and fitted well with the findings of Li et al (2011) [36] and Hu et al (2016) [29], but differed from that of moderately resistant reported by Qiu et al (2011) [37] and Chen et al…”

Section: Discussionsupporting

confidence: 88%

“…The average resistance score of each line was calculated as the weighted average of the scores for all the seedlings tested. Based on the resistance level evaluation standard of Hu et al (2016) [29], the rice lines were classified as highly resistant(HR), resistant(R), moderately resistant(MR), moderately susceptible(MS), susceptible(S), and highly susceptible(HS) corresponding to 0-0.9, 1.0-2.9, 3.0-4.9, 5.0-6.9, 7.0-8.9, and 9, respectively.…”

Section: Evaluation Of Resistance and Host Choice Of Rice To Bphmentioning

confidence: 99%

“…The resistance gene of Bph14 has been cloned and its resistance mechanisms had also been identified [10]. Now, resistance genes of Bph14 and Bph15are been applied extensively in rice breeding through molecular marker-assisted selection (MAS) [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

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Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Qiu

Wang²,

Shi³

et al. 2020

Agriculture

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The brown planthopper(BPH), Nilaparvata lugens, is the most destructive insect pest in rice production worldwide. The development and cultivation of BPH-resistant varieties is the most economical and efficient strategy to overcome the destruction caused by BPH. The modified bulk seedling test method was used to identify the BPH resistance level and host feeding choice of rice lines of Liangyou8676(Bph14/Bph15), Bph68S(Bph14/Bph15), RHT(Bph3), Fuhui676, and TN1 on BPH. In the meantime, the population, survival and emergence rate, developmental duration, honeydew excretion, female ratio and brachypterous ratio of adults were used as indicators to detect the antibiosis effects of the different rice lines. The results showed that the resistance levels of RHT, Bph68S, Liangyou8676, Fuhui676, and TN1 to BPH were HR, R, MR, S and HS, respectively. The host choice implied that BPH was more inclined to feeding on rice plants with a lower resistance. An analysis of the antibiosis activity of rice lines RHT, BPh68S, and Liangyou8676 carrying resistance genes indicated a significant reduction in the population growth rate, survival and emergence rate of BPH nymphs, significant delay in the developmental duration of nymphs, reduced honeydew excretion of females, decreased female ratio, and a decreased brachypterous ratio of females and males, when compared with rice carrying no BPH-resistant genes.

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Section: Discussionsupporting

confidence: 88%

Section: Evaluation Of Resistance and Host Choice Of Rice To Bphmentioning

confidence: 99%

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Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Qiu

Wang²,

Shi³

et al. 2020

Agriculture

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“…Because the success of host plant resistance is limited by herbivore adaptation to resistant varieties (often referred to as ‘virulence adaptation’), there has been a clear focus on selecting recognized ‘broad spectrum’ genes/loci, or on pyramiding a number of genes/loci into a single genotype to prolong resistance durability ( Myint et al, 2012 ; Wang et al, 2017 ; Horgan et al, 2018a ). A number of research groups have now developed multiple near-isogenic rice lines with a range of different resistance genes either singly introgressed (monogenic) or pyramided (polygenic) into high-yielding varieties, each with >85% recovery of the recurrent parent genes ( Fujita et al, 2010 ; Hu et al, 2016b ; Jena et al, 2017 ). Polygenic resistance to herbivores is predicted to be stronger ( Fujita et al, 2010 ; Hu et al, 2013 ; Asano et al, 2015 ), have a broader spectrum ( Hu et al, 2016b ; Fan et al, 2017 ), have greater stability ( Ali et al, 2006 ), and be more durable ( Myint et al, 2012 ; Wang et al, 2017 ; Horgan et al, 2018a ) than monogenic resistance.…”

Section: Introductionmentioning

confidence: 99%

“…A number of research groups have now developed multiple near-isogenic rice lines with a range of different resistance genes either singly introgressed (monogenic) or pyramided (polygenic) into high-yielding varieties, each with >85% recovery of the recurrent parent genes ( Fujita et al, 2010 ; Hu et al, 2016b ; Jena et al, 2017 ). Polygenic resistance to herbivores is predicted to be stronger ( Fujita et al, 2010 ; Hu et al, 2013 ; Asano et al, 2015 ), have a broader spectrum ( Hu et al, 2016b ; Fan et al, 2017 ), have greater stability ( Ali et al, 2006 ), and be more durable ( Myint et al, 2012 ; Wang et al, 2017 ; Horgan et al, 2018a ) than monogenic resistance. Ecological theory suggests that strong resistance is also likely to have associated costs as plants must balance growth, reproduction and defence (including maintenance) to achieve optimal life-history strategies in their respective niches ( Herms and Mattson, 1992 ; Horgan et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Unanticipated benefits and potential ecological costs associated with pyramiding leafhopper resistance loci in rice

Horgan

Almazan

Quan³

et al. 2019

Crop Protection

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We tested the hypotheses that increasing the number of anti-herbivore resistance loci in crop plants will increase resistance strength, increase the spectrum of resistance (the number of species affected), and increase resistance stability. We further examined the potential ecological costs of pyramiding resistance under benign environments. In our experiments, we used 14 near-isogenic rice lines with zero (T65: recurrent parent), one, two or three resistance loci introgressed through marker-assisted selection. Lines with two or more loci that were originally bred for resistance to the green rice leafhopper, Nephotettix cincticeps, significantly reduced egg-laying by the green leafhopper, N. virescens. Declines in egg-number and in nymph weight were correlated with the numbers of resistance loci in the rice lines. To test the spectrum of resistance, we challenged the lines with a range of phloem feeders including the zig-zag leafhopper, Recilia dorsalis, brown planthopper, Nilaparvata lugens, and whitebacked planthopper, Sogatella furcifera. There was an increase in the number of tested species showing significant declines in egg-laying and nymph survival on lines with increasing numbers of loci. In a screen house trial that varied rates of nitrogenous fertilizer, a line with three loci had stable resistance against the green leafhopper and the grain yields of infested plants were maintained or increased (overcompensation). Under benign conditions, plant growth and grain yields declined with increasing numbers of resistance loci. However, under field conditions with natural exposure to herbivores, there were no significant differences in final yields. Our results clearly indicate the benefits, including unanticipated benefits such as providing resistance against multiple herbivore species, of pyramiding anti-herbivore resistance genes/loci in crop plants. We discuss our results as part of a review of existing research on pyramided resistance against leafhoppers and planthoppers in rice. We suggest that potential ecological costs may be overcome by the careful selection of gene combinations for pyramiding, avoidance of high (potentially redundant) loci numbers, and introgression of loci into robust plant types such as hybrid rice varieties.

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Evaluation of Guangxi common wild rice for resistance to brown planthopper using a new stem evaluation method

et al. 2021

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Brown planthopper [BPH, Nilaparvata lugens (Stål)] is considered one of the most important pests of rice (Oryza sativa L.), which poses a serious threat to rice production. Identifying resistant Oryza germplasm can provide reliable accessions for breeding BPH resistant rice cultivars. In this study, the stem evaluation method (SEM) was first applied to identify the BPH resistance of 1,221 accessions of common wild rice (O. rufipogon Griff.) collected from three different regions of Guangxi Province, China. From this screening, 58 BPH resistant accessions were screened a second time, with 33 accessions ultimately identified as stable, highly resistant germplasm as confirmed by a third identification at the adult-plant stage. The distribution of the 58 BPH-resistant common wild rice accessions varies significantly from region to region. Genotypic analyses based on 42 simple sequence repeat (SSR) markers revealed that these 58 BPH-resistant accessions were genetically diverse, reflecting the rich genetic diversity reported in Guangxi common wild rice. Furthermore, results verified that the SEM is efficient for rapid and accurate screening of BPH-resistant germplasm, especially when a limited number of seeds are available or elite breeding lines need to be screened immediately. Also, SEM is the best method for evaluating BPH resistance at the adult stage because fewer insects are needed, and it is possible to repeat the evaluation in the same crop season. The 33 resistant rice accessions are a potential source of novel BPH resistance genes for developing cultivars with improved BPH resistance.

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Application of marker‐assisted backcross to introgress Bph3, Bph14 and Bph15 into an elite indica rice variety for improving its resistance to brown planthopper

Cited by 20 publications

References 42 publications

Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Antibiosis Effects of Rice Carrying Bph14 and Bph15 on the Brown Planthopper, Nilaparvata lugens

Unanticipated benefits and potential ecological costs associated with pyramiding leafhopper resistance loci in rice

Evaluation of Guangxi common wild rice for resistance to brown planthopper using a new stem evaluation method

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