Technological pedagogical content knowledge (TPACK) is the knowledge of abilities that teachers need to have in facing 21st-century learning. Knowledge domains in the TPACK framework include TK, PK, CK, TPK, TCK, PCK, and TPACK. The capability of the teacher’s TPACK to rate with a valid instrument design is needed. This article discusses the validation and validity of TPACK instruments using the Rasch model analysis. The TPACK instrument used was Two-Tier Multiple Choice (TTMC), in which the tier-one was as the material foundation, and the tier-two was as supporting reasons. The validation was carried out by seven education experts, namely teachers who had a working period of more than 15 years, one material expert, and one material expert as well as a test construction expert in the Forum Group Discussion (FGD). There were 21 item TTMC questions analyzed. The analyzed using the Rasch model application. The results are that all TTMC TPACK questions are valid. The instrument can be to assess the ability of pre-service chemistry teachers TPACK in future studies.